Abstract：
:A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating agents, detergents and cell-wall-hydrolyzing chitinase. Considerable amount of exopolysaccharides from fungal biomass was removed with the use of 1% CTAB wash buffer followed by centrifugation. The DNA varied in terms of yield and quality. For the DNA extraction use of 2% SDS in extraction buffer worked best for Pycnoporus cinnabarinus, Cyathus bulleri, Cyathus striatus and Cyathus stercoreus, while 2% CTAB worked best for Phanerochaete chrysosporium and Pleurotus ostreatus. Elimination of phenol and use of absolute ethanol for precipitating DNA resulted in good yield and quality of DNA. This DNA was amenable to restriction endonuclease digestion.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kuhad RC,Kapoor RK,Lal Rdoi
10.1007/BF02931383keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
112-6issue
2eissn
0015-5632issn
1874-9356journal_volume
49pub_type
杂志文章abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931412
更新日期：2005-01-01 00:00:00
abstract：:A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0660-x
更新日期：2019-05-01 00:00:00
abstract：:The soil microbial population, determined over a period of 19 months and the elemental chemical composition measured by X-ray fluorescence techniques were followed in two uncultivated salt-affected areas of Greece, namely Lantza and Klidi. The ecological conditions at Lantza remained stable during the period of study ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877387
更新日期：1983-01-01 00:00:00
abstract：:When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879490
更新日期：1983-01-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during infection, including induction of the cell cycle G2 arrest, and cell death in both human cells and the fission yeast Schizosaccharomyces pombe. We show that treament of exponential-pha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931584
更新日期：2006-01-01 00:00:00
abstract：:Among 18 tested strains of Claviceps spp., 7 produced significant amounts of exocellular polysaccharide (EPS). The maximum production of EPS was found in fermentation broth of Claviceps viridis. The kinetics of growth, substrate consumption, and EPS production in the batch, aerobic, submerged culture of this fungus we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02993470
更新日期：2003-01-01 00:00:00
abstract：:Contemporary aspects of cerebrospinal fluid analysis are discussed, including the relationship to neuro-infective, autoimmune and other neurological diseases. The actual state of cerebrospinal fluid microbiological and cytological investigation and analysis of cerebrospinal fluid protein fractions are described in det...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02931522
更新日期：2003-01-01 00:00:00
abstract：:Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of t...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814620
更新日期：1996-01-01 00:00:00
abstract：:Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being som...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885600
更新日期：1977-01-01 00:00:00
abstract：:Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:The cellular immune response (MIF, E-rosette formation and changes in nucleolar morphology of lymphocytes) was followed as related to age and antigenic stimulation. MIF in healthy infants increased from the 2nd to the 12th week of life as compared with the first week, probably due to BCG vaccination. The total and act...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876400
更新日期：1980-01-01 00:00:00
abstract：:In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0666-4
更新日期：2019-07-01 00:00:00
abstract：:Killer strains of Saccharomyces cerevisiae producing killer toxin K1 kill sensitive cells but are resistant to their own toxin. It is assumed that in the producer, an effective interaction between the external toxin and its plasma membrane receptor or the final effector is not possible on the grounds of a conformation...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932122
更新日期：2006-01-01 00:00:00
abstract：:The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02816234
更新日期：1999-01-01 00:00:00
abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0036-3
更新日期：2009-01-01 00:00:00
abstract：:Glutamate was converted to glutamine by Corynebacterium glutamicum permeabilized by ethanol (10%). ATP was supplied to the reaction by dried Saccharomyces cerevisiae or regenerated by acetyl kinase. High glutamine synthetase activity in C. glutamicum was induced by cultivation of the microorganism in media containing ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816248
更新日期：1999-01-01 00:00:00
abstract：:Cardiolipin synthesis was studied during the aerobic synchronous growth of aerobically grown yeast Saccharomyces cerevisiae. The time course of the synthesis was stepwise and the rise in cardiolipin level in cells coincided in time with the increase in cytochrome oxidase activity. This finding supports the notion of d...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876990
更新日期：1977-01-01 00:00:00
abstract：:Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0595-2
更新日期：2018-09-01 00:00:00
abstract：:Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931594
更新日期：2006-01-01 00:00:00
abstract：:Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0092-3
更新日期：2012-01-01 00:00:00
abstract：:Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0592-5
更新日期：2018-07-01 00:00:00
abstract：:Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931454
更新日期：2005-01-01 00:00:00
abstract：:Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated fro...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0047-0
更新日期：2010-07-01 00:00:00
abstract：:Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02935823
更新日期：1991-01-01 00:00:00
abstract：:Hydroxy, n-saturated, branched, dioic, and unsaturated fatty acids in six freshwater wild cyanobacteria (Chroococcus minutus, Lyngbya ceylanica, Merismopedia glauca, Nodularia sphaerocarpa, Nostoc linckia, and Synechococcus aeruginosus) collected from different lakes and springs of Israel have been identified by gas c...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931279
更新日期：2003-01-01 00:00:00
abstract：:The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements we...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02925901
更新日期：1988-01-01 00:00:00
abstract：:We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814307
更新日期：1994-01-01 00:00:00
abstract：:The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-011-0056-7
更新日期：2011-07-01 00:00:00
abstract：:Oxygen uptake by the spores of Fusarium moniliforme, F. oxysporum, F. semitectum, F. solani, Mucor racemosus and Trichoderma viride was increased in the presence of volatile substances extracted from Origanum majorana and Ocimum basilicum. This increase was greater in the presence of volatile substances from O. basili...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02885580
更新日期：1978-01-01 00:00:00
abstract：:Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927365
更新日期：1981-01-01 00:00:00
