Abstract：
:A series of experiments were performed with P. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the beta-lactam antibiotics. The constitutive beta-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol. The strain was also submitted to a series of treatments with Tris and Tris-EDTA to disrupt the outer membrane. The treated cells demonstrated a ten-fold reduction in the MIC with cloxacillin, six-fold with penicillin, and five-fold with oxacillin. At least two different biochemical mechanisms were responsible for the resistance to the beta-lactams studied which could be important in the prevalence of P. aeruginosa in nosocomial infections.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Soto UL,Amador SM,Ramos FJ,Baca BEdoi
10.1007/BF02877216subject
Has Abstractpub_date
1987-01-01 00:00:00pages
290-6issue
4eissn
0015-5632issn
1874-9356journal_volume
32pub_type
杂志文章abstract：:One-hundred patients with candidosis of the skin, mucous membranes and nail plates (confirmed by cultivation) were examined. Topical or systemic antimycotic treatment was successful in 58 patients. After a complete evaluation, 42 patients were found to suffer from factors supporting candidosis--diabetes mellitus (12),...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815627
更新日期：2001-01-01 00:00:00
abstract：:Genetic mapping of the chromosome of the bacteriophage PS8 of Agrobacterium tumefaciens using temperature sensitive (ts) mutants of the bacteriophage is described. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927304
更新日期：1979-01-01 00:00:00
abstract：:Lactobacillus bulgaricus 59 and L. casei RTS and their respective nitrosoguanidine-induced mutants were estimated for titratable and volatile acidities, production of biacetyl and acetoin and for proteolytic activity. Both the mutants showed a marked increase in proteolytic activity as compared to the parents. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02897216
更新日期：1980-01-01 00:00:00
abstract：:Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibrisolvens. Sequences of 16S rDNA were obtained from our isolates (serving as a source of data for primer design) and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were des...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02825888
更新日期：2001-01-01 00:00:00
abstract：:Enterococcus faecalis is a ubiquitous bacterium of the gut that is observed in persistent periradicular infections. Its pathogenicity is associated with biofilm formation and the ability to survive under nutrient-poor (starvation) conditions. However, characteristics of chemical composition of biofilm cells developed ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0319-1
更新日期：2014-09-01 00:00:00
abstract：:A 6.5 kb DNA fragment containing the gene (thrC) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the DNA of Bacillus sp. ULM1 by complementation of Escherichia coli and Brevibacterium lactofermentum thrC auxotrophs. Complementation studies showed that the thrB g...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818515
更新日期：1995-01-01 00:00:00
abstract：:Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-015-0396-9
更新日期：2015-11-01 00:00:00
abstract：:The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC p...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877396
更新日期：1982-01-01 00:00:00
abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931412
更新日期：2005-01-01 00:00:00
abstract：:Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814645
更新日期：1994-01-01 00:00:00
abstract：:Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:Resistance determinants for HgCl2 and CdCl2 were eliminated along with a number of antibiotic resistance factors from Acinetobacter lwoffi and Micrococcus sp. at 44 degrees C. These organisms were orginally resistant to HgCl2, merbromin, CdCl2, Pb(NO3)2, benzylpenicillin, erythromycin, carbenicillin, tetracycline and ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02925907
更新日期：1988-01-01 00:00:00
abstract：:Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931422
更新日期：2005-01-01 00:00:00
abstract：:The aim of this study was to determine the prevalence of Listeria sp. and Listeria monocytogenes in soil samples with reference to type of fertilizers (natural and artificial) and distance from places intensively exploited by men, as well as to determine the relationship between the presence of L. monocytogenes in the...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0260-8
更新日期：2014-01-01 00:00:00
abstract：:Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817448
更新日期：2000-01-01 00:00:00
abstract：:In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complem...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00682-7
更新日期：2019-07-01 00:00:00
abstract：:This review discusses methods for the single-chain antibody fragment ($cFv) generation and scFv expression systems, and describes potential applications of scFv in the therapy of viral diseases and cancer, with emphasis on intracellularly expressed scFvs (intrabodies), application of scFvs in detection and diagnostics...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02993480
更新日期：2003-01-01 00:00:00
abstract：:The levels of anti-Candida antibodies were determined in experimental animals immunized with 4 different yeast doses (0.3, 0.6, 1.2, 2.4 x 10(8) CFU) at weekly intervals. After immunization (sampling intervals 1, 2, and 3 months), the intravenous blood was examined for the presence of serum anti-Candida antibodies. Af...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931340
更新日期：2003-01-01 00:00:00
abstract：:The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876963
更新日期：1976-01-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during infection, including induction of the cell cycle G2 arrest, and cell death in both human cells and the fission yeast Schizosaccharomyces pombe. We show that treament of exponential-pha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931584
更新日期：2006-01-01 00:00:00
abstract：:The yellow-green alga Trachydiscus minutus (class Xanthophyta) was cultivated in a standard medium and in media without sulfur and nitrogen. Its yield after a 16-d cultivation reached 13 g dry mass per 1 L medium. The content of oligoenoic ('polyenoic') fatty acid (PUFA), i.e. eicosapentaenoic (EPA), was in excess of ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0039-0
更新日期：2010-05-01 00:00:00
abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0036-3
更新日期：2009-01-01 00:00:00
abstract：:Biofilms represent mixed communities present in a diverse range of environments; however, their utility as inoculants is less investigated. Our investigation was aimed towards in vitro development of biofilms using fungal mycelia (Trichoderma viride) as matrices and nitrogen-fixing and P-solubilizing bacteria as partn...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0154-1
更新日期：2012-09-01 00:00:00
abstract：:There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0495-x
更新日期：2017-05-01 00:00:00
abstract：:Spatial distribution of ectomycorrhizae-associated basidiomycetes was determined in oakbirch forest using terminal restriction fragment length polymorphism (T-RFLP) analysis. The data were correlated with actual soil humidity, pH, electric conductivity of the soil extract, absorbance A(465) and A(665) of water and alk...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0076-8
更新日期：2010-09-01 00:00:00
abstract：:The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a ly...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816944
更新日期：1997-01-01 00:00:00
abstract：:Thirty-four thiosemicarbazones and S-alkyl thiosemicarbazones, and some of their Zn(II) and Pd(II) complexes were obtained and purified to investigate antimicrobial activity. MIC values of the compounds were determined by the disc diffusion method against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pse...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932132
更新日期：2007-01-01 00:00:00
abstract：:Analysis of actA gene sequence polymorphism has been shown to be an effective and relatively inexpensive method for subtyping Listeria monocytogenes isolates, allowing the division of the population of this species into two deeply separate lineages. This sequence-based method as well as PCR-mediated fingerprinting wer...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0003-z
更新日期：2009-01-01 00:00:00
abstract：:The geographical distribution of Bacillus anthracis strains and isolates bearing some of the same genetic markers as the Amerithrax Ames isolate was examined and evaluated. At least one mechanism for the horizontal movement of genetic markers was shown amongst isolates and closely related species and the effect of suc...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0074-2
更新日期：2008-01-01 00:00:00
abstract：:The mean lengths of G1, S, G2 and M phases of the cell cycle were determined on the basis of the population distribution of Candida utilis grown in a continuous culture under steady-state conditions by using an original mathematical method. The length of the G2 phase was proportional to that of G1; the length of M was...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821281
更新日期：1990-01-01 00:00:00
