The production and application of single-chain antibody fragments.

abstract：:Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...

journal_title：Folia microbiologica

authors： Bhatnagar M,Bhatnagar A

更新日期：2004-01-01 00:00:00

abstract：:Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...

journal_title：Folia microbiologica

authors： Spanová A

更新日期：1992-01-01 00:00:00

abstract：:The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N'-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixt...

journal_title：Folia microbiologica

authors： Nováková Z,Surín S,Blasko J,Majerník A,Smigán P

更新日期：2008-01-01 00:00:00

abstract：:The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 8201 fluorescence microscopes at various excitations. The strongest AF of hyphae was found in minimal medium with glucose, where the hyphae exhibited green...

journal_title：Folia microbiologica

authors： Zižka Z,Vêtrovský T,Gabriel J

更新日期：2010-11-01 00:00:00

abstract：:Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metaboli...

journal_title：Folia microbiologica

authors： Betina V

更新日期：1980-01-01 00:00:00

abstract：:Candida bracarensis is an emerging cryptic species within the Candida glabrata clade. To date, little is known about its epidemiology, virulence, and antifungal susceptibility. This study documents the occurrence of C. bracarensis for the first time in Mexico and focuses on its in vitro production of hydrolytic enzyme...

journal_title：Folia microbiologica

authors： Treviño-Rangel RJ,Espinosa-Pérez JF,Villanueva-Lozano H,Montoya AM,Andrade A,Bonifaz A,González GM

更新日期：2018-07-01 00:00:00

abstract：:Adhesion of group B streptococci to human epithelial vaginal and buccal cells proceeded in three phases which differed qualitatively. Maximum adhesion took place within 10 min of interaction, during the second phase (10-15 min), the percentage of adherent cells decreased significantly (P less than 0.05) whereas during...

journal_title：Folia microbiologica

authors： Kubín V,Jirásková Z,Franĕk J

更新日期：1983-01-01 00:00:00

abstract：:Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated fro...

journal_title：Folia microbiologica

authors： Bełzecki G,Miltko R,Kwiatkowska E,Michałowski T

更新日期：2010-07-01 00:00:00

abstract：:The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....

journal_title：Folia microbiologica

authors： Bĕhal V

更新日期：2003-01-01 00:00:00

abstract：:Biodegradation of two amphiphilic "soft" antimicrobially active derivatives of lauric (dodecanoic) acid, a quaternary ammonium salt and an amine oxide bearing an amide or ester group, was followed using microorganisms from activated sludge. Primary biodegradation was determined by ion-selective electrodes, total biode...

journal_title：Folia microbiologica

authors： Cupková V,Sirotková L,Mlynarcík D,Devínsky F,Lacko I,Kovacková Z

更新日期：1993-01-01 00:00:00

abstract：:Virulence factors were compared in 15 Aeromonas spp. isolated from faeces of patients with Aeromonas-associated gastroenteritis and in 81 strains isolated from food. Strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. However, 88.8% of Ae...

journal_title：Folia microbiologica

authors： Pin C,Marín ML,Selgas MD,García ML,Tormo J,Casas C

更新日期：1994-01-01 00:00:00

abstract：:Mycobacterium microti-infected mouse peritoneal macrophages produced high amounts of prostaglandin E2 (PGE2) and nitric oxide (NO) when activated with interferon-gamma (IFN-gamma). In order to understand the relation between PGE2 and NO production and the expression of interleukin-12 (IL-12), interleukin-10 (IL-10) an...

journal_title：Folia microbiologica

authors： Mittal J,Dogra N,Vohra H,Majumdar S

更新日期：2001-01-01 00:00:00

abstract：:The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative exp...

journal_title：Folia microbiologica

authors： Lee YM,Lee H,Heo YM,Lee H,Hong JH,Kim JJ

更新日期：2017-05-01 00:00:00

abstract：:When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthe...

journal_title：Folia microbiologica

authors： Kucerová H,Vĕchet B

更新日期：1982-01-01 00:00:00

abstract：:The assumption that the pyruvate decarboxylase activity of Saccharomyces carlsbergensis is the main limiting factor determining the formation rate and the total amount of d(--)-l-hydroxy-l-phenyl-propanone (phenylacetylcarbinol, PAC) produced was not confirmed. An increase of about 30% of the total amount of the PAC p...

journal_title：Folia microbiologica

authors： Vojtísek V,Netrval J

更新日期：1982-01-01 00:00:00

abstract：:Biological activity of secondary metabolites produced by a plant-growth-promoting Pseudomonas fluorescens was evaluated. The strain produced antibiotics phenazine (PHE), 2,4-diacetylphloroglucinol (PHL) and siderophore pyoverdin (PYO) in standard King's B and succinic acid media, respectively. After extraction, PYO wa...

journal_title：Folia microbiologica

authors： Boruah HP,Kumar BS

更新日期：2002-01-01 00:00:00

abstract：:Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins of Clostridium tetani, C. botulinum; lethal factors of C. novyi an...

journal_title：Folia microbiologica

authors： Vraný B,Hnátková Z,Lettl A

更新日期：1988-01-01 00:00:00

abstract：:White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. ...

journal_title：Folia microbiologica

authors： Kahraman S,Yeşilada O

更新日期：2001-01-01 00:00:00

abstract：:Proteinase production by Lactobacillus murinus was influenced by temperature, glucose concentration, initial pH and nitrogen sources. Maximum proteinase production occurred at 45 degrees C, pH 6.6 and with 0.5% (W/V) glucose. Tryptone, peptone and gelatin inhibited it. ...

journal_title：Folia microbiologica

authors： Strasser de Saad AM,Manca de Nadra MC,Pesce de Ruiz Holgado AA,Oliver G

更新日期：1987-01-01 00:00:00

abstract：:NaN3 was found to inhibit transformation but not the irreversible binding of donor 3H-DNA in competent cells of the original low-transformable strain Bacillus subtilis 168 trp2. Addition of NaN3 to cells of two mutants Bacillus subtilis HT39 and HT46 with an increased transformability decreased substantially the irrev...

journal_title：Folia microbiologica

authors： Tichý P,Jandová Z

更新日期：1977-01-01 00:00:00

abstract：:Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...

journal_title：Folia microbiologica

authors： Hubácek J

更新日期：1992-01-01 00:00:00

abstract：:We report the complete nucleotide sequence and characterization of a small cryptic plasmid of Moraxella macacae 0408225, a newly described bacterial species within the family Moraxellaceae and a causative agent of epistaxis in macaques. The complete nucleotide sequence of the plasmid pMoma1 was determined and found to...

journal_title：Folia microbiologica

authors： Whitehouse CA,Ladner JT,Palacios GF

更新日期：2015-05-01 00:00:00

abstract：:The effect of the newly synthesized phosphonic compound dibutyl 2-octylamino-2-propanephosphonate (DBOP) on the growth of the aquatic plant Spirodela oligorrhiza and stability of red blood cells (RBC) and planar lipid membranes (BLM) was studied to determine its physiological activity and, if possible, correlate this ...

journal_title：Folia microbiologica

authors： Kleszczyńska H,Sarapuk J,Bielecki K,Grzyś E,Witek S

更新日期：2000-01-01 00:00:00

abstract：:The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RN...

journal_title：Folia microbiologica

authors： Wickhorst JP,Hassan AA,Sheet OH,Eisenberg T,Sammra O,Alssahen M,Lämmler C,Prenger-Berninghoff E,Zschöck M,Timke M,Abdulmawjood A

更新日期：2018-01-01 00:00:00

abstract：:The minimum inhibitory concentrations (MICs) of 24 antibiotics were determined for 45 Stenotrophomonas maltophilia strains by the microdilution method at 37 and 30 degrees C (after 24 h and 48 h of incubation). The isolates were obtained from mouth swabs and pus of 116 captive snakes whereas the identical strains (bas...

journal_title：Folia microbiologica

authors： Hejnar P,Kolár M,Sauer P

更新日期：2010-01-01 00:00:00

abstract：:The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was dete...

journal_title：Folia microbiologica

authors： Krutkiewicz A,Klimuszko D

更新日期：2010-03-01 00:00:00

abstract：:Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...

journal_title：Folia microbiologica

authors： Mullié C,Romond MB,Izard D

更新日期：2006-01-01 00:00:00

abstract：:A soil streptomycete degraded hair, silk, wool, feather and leather which were collected from solid wastes. The organism was identified taxonomically and designated Streptomyces sp. A956. It degraded leather to the maximum extent and solubilized 35.9% of the total nitrogen, 2.32 mg of glycine equivalent amino nitrogen...

journal_title：Folia microbiologica

authors： De S,Chandra AL

更新日期：1979-01-01 00:00:00

abstract：:Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...

journal_title：Folia microbiologica

authors： Christian VV,Shrivastava R,Novotný C,Vyas BR

更新日期：2003-01-01 00:00:00

abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...

journal_title：Folia microbiologica

authors： Folwarczna J,Plchová H,Moravec T,Hoffmeisterová H,Dedic P,Cerovská N

更新日期：2008-01-01 00:00:00