Abstract:
:The gene corresponding to the recently identified whiB-paralogous gene wblE in S. coelicolor was found after sequencing the downstream region of the stress-response sporulation-specific sigma-factor gene, sigH, in S. coelicolor A3(2). Sequence analysis has revealed an ORF exhibiting high similarity to sporulation transcription factors WhiB and WhiD. A stable null mutant of the wblE gene was obtained by integrative transformation, via double cross-over. Disruption of the S. coelicolor wblE gene appeared to have no obvious effect on growth, morphology, differentiation, and production of the pigmented antibiotics actinorhodin and undecylprodigiosin. Expression of the wblE gene was investigated during differentiation by S1 nuclease mapping, using RNA prepared from S. coelicolor A3(2) and its isogenic sigF and sigH mutants grown to various developmental stages. A single promoter was identified upstream of the wblE coding region. The wblEp promoter was induced at the beginning of aerial mycelium formation and its activity decreased later in differentiation. No differences in expression of the wblEp promoter were detected in S. coelicolor A3(2) mutants in sigF and sigH genes for sporulation-specific sigma factors. Sequence of the wblEp promoter showed partial similarity to the consensus sequence of the extracytoplasmic sigma factors.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Homerová D,Sevcíková J,Kormanec Jdoi
10.1007/BF02931330keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
489-95issue
4eissn
0015-5632issn
1874-9356journal_volume
48pub_type
杂志文章abstract::Using fermentation with a selected strain of Acetomonas oxydans it was possible to convert 2-deoxy-2-fluro-D-glucitol to 5-deoxy-5-fluoro-L-sorbose, in agreement with Bertrand's and Hudson's rule. The last-named compound was isolated in a yield of 88%. Both compounds were little toxic against Acetomonas oxydans. ...
journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
pub_type: 杂志文章
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877669
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877085
更新日期:1975-01-01 00:00:00
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926452
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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journal_title:Folia microbiologica
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pub_type: 杂志文章
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journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816213
更新日期:1999-01-01 00:00:00
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