Abstract:
:Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Volvocales). Immunofluorescence and immunoblotting revealed that some of the plectin-like proteins were present in perinuclear region or localized near the cell wall, probably being attached to the cytoplasmic membrane.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Hendrychová J,Vítová M,Bisová K,Wiche G,Zachleder Vdoi
10.1007/BF02818794keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
535-9issue
5eissn
0015-5632issn
1874-9356journal_volume
47pub_type
杂志文章abstract::beta-Glucosidase from the fungus Thermoascus aurantiacus grown on semi-solid fermentation medium (using ground corncob as substrate) was partially purified in 5 steps--ultrafiltration, ethanol precipitation, gel filtration and 2 anion exchange chromatography runs, and characterized. After the first anion exchange chro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818672
更新日期:2002-01-01 00:00:00
abstract::Frequency of heterocytes and nitrogenase activity (NA) under light and dark cultivation conditions was determined in 12 cyanobacterial strains isolated from various soil habitats. In spite of a high variability, significant differences in NA among the strains were found in response of light and dark cultivation. Relat...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931606
更新日期:2004-01-01 00:00:00
abstract::The green alga Scenedesmus intermedius was grown in synchronous culture under manganese or calcium deficiency for six successive generations. The growth rate, pigment and protein contents gradually decreased in comparison with the control. In Mn-deficient cells, the rate of oxygen evolution was sharply decreased. This...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817561
更新日期:2000-01-01 00:00:00
abstract::Mutations occurring in viral polymerase gene of hepatitis B virus (HBV) due to the use of nucleos(t)id analogs reduce the activity of the drugs by causing antiviral resistance. In this study, it was aimed to evaluate mutations responsible for drug resistance and drug resistance mutation rates in patients followed up b...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0650-z
更新日期:2019-03-01 00:00:00
abstract::Thirty-three fully breast-fed infants aged between 1 and 12 weeks were screened for bifidobacteria in feces. Bifidobacteria counts in most fecal samples determined both by TPY agar and FISH procedure ranged from 10(8) to 10(11) CFU/g. Three infants did not contain any bifidobacteria in their fecal samples. One child w...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931404
更新日期:2004-01-01 00:00:00
abstract::The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N'-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixt...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0034-x
更新日期:2008-01-01 00:00:00
abstract::A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to d...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932086
更新日期:2007-01-01 00:00:00
abstract::Nalidixic acid was used for describing more accurately the terminal replication region of the Mycobacterium phlei chromosome. Cell division in synchronized cultures was not sensitive to this acid any more between 185-190 min, i.e. about 10 min after replication of the ser gene, the last of 24 genes of the replication ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927251
更新日期:1981-01-01 00:00:00
abstract::The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02818608
更新日期:1998-01-01 00:00:00
abstract::In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-T...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-016-0450-2
更新日期:2016-09-01 00:00:00
abstract::One-week dextran sulfate treatment of conventional (CV) immunodeficient (SCID) mice gave rise to acute colitis in the colon mucosa; germ-free (GF) SCID mice did not exhibit any changes in colon morphology. Dextran sulfate application to CV immunocompetent (BALB/c) mice did induce substantial changes in the colon mucos...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818004
更新日期:2001-01-01 00:00:00
abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0124-7
更新日期:2012-07-01 00:00:00
abstract::We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931538
更新日期:2004-01-01 00:00:00
abstract::Proteinase production by Lactobacillus murinus was influenced by temperature, glucose concentration, initial pH and nitrogen sources. Maximum proteinase production occurred at 45 degrees C, pH 6.6 and with 0.5% (W/V) glucose. Tryptone, peptone and gelatin inhibited it. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877263
更新日期:1987-01-01 00:00:00
abstract::The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements we...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02925901
更新日期:1988-01-01 00:00:00
abstract::The diversity and structure of bacterial and actinobacteral diversity and communities were determined in a metallophytic grassland soil from an upland site in northern England. The community profiles were subjected to multivariate analyses using correspondence and cluster analyses. The total bacterial community divers...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0042-5
更新日期:2009-01-01 00:00:00
abstract::Thirty-four thiosemicarbazones and S-alkyl thiosemicarbazones, and some of their Zn(II) and Pd(II) complexes were obtained and purified to investigate antimicrobial activity. MIC values of the compounds were determined by the disc diffusion method against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pse...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932132
更新日期:2007-01-01 00:00:00
abstract::Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simp...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0165-y
更新日期:2012-11-01 00:00:00
abstract::The biological activity and the presence of genes sfp and ituD (surfactin and iturin A) among Bacillus strains isolated from the Amazon basin were determined. Bacillus spp. were tested for hemolytic activity and inhibition of fungal growth by agar plate assays in parallel with PCR for identification of sfp and ituD ge...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0056-7
更新日期:2011-07-01 00:00:00
abstract::A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931464
更新日期:2005-01-01 00:00:00
abstract::Diseases of the central nervous system (CNS) mean for the human organism a potentially dangerous situation. An investigation of cerebrospinal fluid (CSF) provides important information about a character of CNS impairment in the decision-making diagnostic and therapeutic algorithm. The authors present a brief overview ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-018-0663-7
更新日期:2019-05-01 00:00:00
abstract::The activities of inorganic pyrophosphatase, thymidine kinase and thymidine phosphorylase were measured in Ter-mutants of E. coli K12 which have a higher or a lower dTTP pool than the parent strain. The levels of inorganic pyrophosphatase and thymidine kinase were changed in the same direction and that of thymidine ph...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884076
更新日期:1983-01-01 00:00:00
abstract::The emergence of spa types and spa-clonal complexes (CC) among clinical methicillin-resistant Staphylococcus aureus isolates collected from the University Clinical Center in Gdańsk between 2008 and 2009 were investigated. Phage typing was used as the initial screening in the study. The basic set of phages and the addi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0148-z
更新日期:2012-05-01 00:00:00
abstract::The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0067-1
更新日期:2008-01-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) isolates contain large genomic segments, termed pathogenicity islands (PAIs), that contribute to their virulence. A total of 150 UPEC and 50 commensal E. coli isolates from outpatients were investigated for antimicrobial susceptibility and the presence of eight PAI markers. One hu...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0433-8
更新日期:2016-05-01 00:00:00
abstract::Infiltration of immune cells into CNS is one of the essential events in multiple sclerosis (MS) development. Adhesion molecules like the intercellular adhesion molecule 1 (ICAM-1) play critical role in this process. Therefore, the ICAM1 gene containing two important single-nucleotide polymorphisms (SNPs) belongs to ca...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0499-6
更新日期:2017-07-01 00:00:00
abstract::A series of experiments were performed with P. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the beta-lactam antibiotics. The constitutive beta-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877216
更新日期:1987-01-01 00:00:00
abstract::We report the cloning and sequence analysis of a gamma-butyrolactone autoregulator regulatory island that includes an sscR gene encoding the gamma-butyrolactone autoregulator receptor from Streptomyces scabies NBRC 12914, a plant pathogenic strain. gamma-Butyrolactone autoregulators trigger secondary metabolism, and s...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0017-y
更新日期:2008-01-01 00:00:00
abstract::Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814437
更新日期:2001-01-01 00:00:00
abstract::The regulation of gene transcription allows yeast cells to respond properly to changing environmental conditions. Several protein complexes take part in this process. They involve RNA polymerase complexes, chromatin remodeling complexes, mediators, general transcription factors and specific transcriptional regulators....
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/s12223-008-0044-8
更新日期:2008-01-01 00:00:00