Abstract：
:We report the cloning and sequence analysis of a gamma-butyrolactone autoregulator regulatory island that includes an sscR gene encoding the gamma-butyrolactone autoregulator receptor from Streptomyces scabies NBRC 12914, a plant pathogenic strain. gamma-Butyrolactone autoregulators trigger secondary metabolism, and sometimes morphological differentiation in the Gram-positive genus Streptomyces through binding to a specific autoregulator receptor. This gene cluster showed close similarity to other regulatory islands of Streptomyces origin that are responsible for the control of secondary metabolism. The recombinant SscR protein expressed in Escherichia coli prefers a gamma-butyrolactone autoregulator containing a long C-2 side chain and beta-hydroxyl group at the C-6 position. An inactivation experiment confirmed that this gamma-butyrolactone autoregulator receptor was involved in secondary metabolism but had no effects on the morphological differentiation. In the sscR-deleted mutant, the binding activity of the gamma-butyrolactone autoregulator was completely abolished, suggesting that its primary role is to detect the presence of an autoregulator in the environment. HPLC analysis of the culture broth showed that some peaks disappeared and new peaks that were not present in the broth of the wild-type strain appeared.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kitani S,Hoshika M,Nihira Tdoi
10.1007/s12223-008-0017-ysubject
Has Abstractpub_date
2008-01-01 00:00:00pages
115-24issue
2eissn
0015-5632issn
1874-9356journal_volume
53pub_type
杂志文章abstract：:Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0004-y
更新日期：2010-01-01 00:00:00
abstract：:The gene corresponding to the recently identified whiB-paralogous gene wblE in S. coelicolor was found after sequencing the downstream region of the stress-response sporulation-specific sigma-factor gene, sigH, in S. coelicolor A3(2). Sequence analysis has revealed an ORF exhibiting high similarity to sporulation tran...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931330
更新日期：2003-01-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:Sixty four biopsy specimens of oral Candida leukoplakias were examined. Histological sections were stained with periodic acid-Schiff (PAS) reagent and by Grocott's silver method. C. albicans isolates were cultivated from all the patients but fungal hyphae were proved histologically only in 23 of them. PAS-positive and...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814437
更新日期：2001-01-01 00:00:00
abstract：:Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814645
更新日期：1994-01-01 00:00:00
abstract：:Three methods were used for the isolation of fungi in the present investigation: the dilution-plate method, hair-baiting technique with horse hairs and the "exposed plate" method. Sabouraud's glucose agar at 28 degrees C was also used as isolation medium. 102 species and 2 species varieties belonging to 36 genera were...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821282
更新日期：1990-01-01 00:00:00
abstract：:Diseases of the central nervous system (CNS) mean for the human organism a potentially dangerous situation. An investigation of cerebrospinal fluid (CSF) provides important information about a character of CNS impairment in the decision-making diagnostic and therapeutic algorithm. The authors present a brief overview ...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0663-7
更新日期：2019-05-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during infection, including induction of the cell cycle G2 arrest, and cell death in both human cells and the fission yeast Schizosaccharomyces pombe. We show that treament of exponential-pha...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931584
更新日期：2006-01-01 00:00:00
abstract：:A proteus strain inhibited mycelial growth of Fusarium oxysporum in vitro. Seed bacterization showed significant plant growth promotion and Fusarium-wilt suppression activity of Phaseolus mungo in a gnotobiotic system. The culture filtrate of this strain exhibited three prominent bands in UV-VIS spectra between 300 an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818723
更新日期：2000-01-01 00:00:00
abstract：:The yellow-green alga Trachydiscus minutus (class Xanthophyta) was cultivated in a standard medium and in media without sulfur and nitrogen. Its yield after a 16-d cultivation reached 13 g dry mass per 1 L medium. The content of oligoenoic ('polyenoic') fatty acid (PUFA), i.e. eicosapentaenoic (EPA), was in excess of ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0039-0
更新日期：2010-05-01 00:00:00
abstract：:Over the years, quinones or its derivatives have been extensively studied due to their broad therapeutic spectrum. However, due to the significant structural differences between the individual naturally occurring quinones, investigation of the precise mechanism of their action is essential. In this context, we have an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-019-00722-2
更新日期：2020-02-01 00:00:00
abstract：:The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931828
更新日期：2006-01-01 00:00:00
abstract：:The induction of tryptophanase was not affected by rifampicin in the rifampicin-resistant mutant of Vibrio el tor while the antibiotic inhibited the induction of tryptophanase in the normal strain at level of ribonucleic acid and protein synthesis. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02877677
更新日期：1977-01-01 00:00:00
abstract：:Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02923516
更新日期：1985-01-01 00:00:00
abstract：:Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. T...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931512
更新日期：2003-01-01 00:00:00
abstract：:The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02816234
更新日期：1999-01-01 00:00:00
abstract：:Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in s...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814494
更新日期：1991-01-01 00:00:00
abstract：:Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02932187
更新日期：2007-01-01 00:00:00
abstract：:Studies on the production of extracellular cellulase in two aquatic hyphomycetes Lunulospora curvula and Flagellospora penicillioides have shown that several factors such as carbon source, nitrogen source, pH and temperature affect the production of the enzyme. Experiments have shown that carboxymethyl cellulose is th...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814357
更新日期：1991-01-01 00:00:00
abstract：:Hemolytic factor production by pathogenic Candida species is considered an important attribute in promoting survival within the mammal host through the ability to assimilate iron from the hemoglobin-heme group. Hemolytic capability has been evaluated for Candida species based on hemolysis zones on plate assay, analysi...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-018-0584-5
更新日期：2018-07-01 00:00:00
abstract：:The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-017-0563-2
更新日期：2018-05-01 00:00:00
abstract：:The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817617
更新日期：2000-01-01 00:00:00
abstract：:Vibrio el tor cells contain a constitutive reductase enzyme which converts nitrofurantoin to an active principle that is responsible for the observed antibacterial activity of the drug. Acquisition of resistance of this strain towards nitrofurantoin is associated with the loss of this reductase. This enzyme is located...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927181
更新日期：1979-01-01 00:00:00
abstract：:Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0273-3
更新日期：2014-03-01 00:00:00
abstract：:Regulatory mechanism of primary metabolism responsible for the biosynthesis of peptide ergot alkaloids was proposed on the basis of experimental results from the production phase of Claviceps purpurea (FR.) Tul. in parasitic and saprophytic cultures. The production-phase metabolism is characterized by uncoupling of gl...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02927365
更新日期：1981-01-01 00:00:00
abstract：:Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-009-0036-3
更新日期：2009-01-01 00:00:00
abstract：:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818608
更新日期：1998-01-01 00:00:00
abstract：:Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simp...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0165-y
更新日期：2012-11-01 00:00:00
abstract：:The majority of antibiotics and substances with diverse biological activity used in medicine are produced by actinomycetes, nonfilamentous bacteria and fungi. Other microorganisms, such as myxobacteria, pseudomonads, nocardias, basidiomycetes, marine microorganisms, enterobacteria, halobacteria, hyperthermophiles etc....
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02993460
更新日期：2003-01-01 00:00:00
abstract：:Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity a...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879491
更新日期：1983-01-01 00:00:00
