Abstract:
:Kocuria spp. are widely distributed in nature. They are Gram-positive, coagulase-negative, coccoid bacteria belonging to the family Micrococcaceae, suborder Micrococcineae, order Actinomycetales, class Actinobacteria. In general, limited knowledge exists concerning the properties associated with the representants of the genus Kocuria, Kocuria kristinae as well. Following our previous results, K. kristinae Kk2014 Biocenol(™) (CCM 8628) was isolated from vagina of a healthy cow. Its taxonomical allottation was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) identification system and phenotypic characteristics. Kk2014 strain showed strong adherence capability to the vaginal mucus, produced organic acids which can play a role in prevention of unsuitable contamination, and showed in vitro antagonistic/antimicrobial activity against strains Arcanobacterium pyogenes CCM 5753, Fusobacterium necrophorum CCM 5982, Streptococcus equi subsp. zooepidemicus CCM 7316, and Gardnerella vaginalis CCM 6221. Antimicrobial activity ranged from 100 to 200 AU/mL, up to 32 mm in size, respectively.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Styková E,Nemcová R,Gancarčíková S,Valocký I,Lauková Adoi
10.1007/s12223-015-0431-xsubject
Has Abstractpub_date
2016-05-01 00:00:00pages
243-8issue
3eissn
0015-5632issn
1874-9356pii
10.1007/s12223-015-0431-xjournal_volume
61pub_type
杂志文章abstract::This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0411-1
更新日期:2016-01-01 00:00:00
abstract::Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. The roles of some of the 14 components of the MTB are now becoming clear. In this review it is proposed that most ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02818976
更新日期:1997-01-01 00:00:00
abstract::Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial stra...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0022-4
更新日期:2011-03-01 00:00:00
abstract::When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthe...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876446
更新日期:1982-01-01 00:00:00
abstract::A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0004-6
更新日期:2011-01-01 00:00:00
abstract::Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0043-z
更新日期:2011-05-01 00:00:00
abstract::Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from h...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02935823
更新日期:1991-01-01 00:00:00
abstract::This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818557
更新日期:1999-01-01 00:00:00
abstract::The synergistic anticandidal activity of three diketopiperazines [cyclo-(L-Pro-L-Leu) (1), cyclo-(D-Pro-L-Leu) (2), and cyclo-(D-Pro-L-Tyr) (3)] purified from a Bacillus sp. N strain associated with entomopathogenic nematode Rhabditis (Oscheius) in combination with amphotericin B and clotrimazole was investigated usin...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-013-0234-x
更新日期:2013-11-01 00:00:00
abstract::An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, per...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02879773
更新日期:1982-01-01 00:00:00
abstract::The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N(2)O and N(2), were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N(2)O production was also ass...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-011-0045-x
更新日期:2011-05-01 00:00:00
abstract::Glutamate was converted to glutamine by Corynebacterium glutamicum permeabilized by ethanol (10%). ATP was supplied to the reaction by dried Saccharomyces cerevisiae or regenerated by acetyl kinase. High glutamine synthetase activity in C. glutamicum was induced by cultivation of the microorganism in media containing ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816248
更新日期:1999-01-01 00:00:00
abstract::Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816213
更新日期:1999-01-01 00:00:00
abstract::Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02887577
更新日期:1987-01-01 00:00:00
abstract::Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0086-6
更新日期:2010-09-01 00:00:00
abstract::Many serious diseases caused by Staphylococcus aureus appear to be associated with biofilms. Therefore, we investigated the biofilm-forming ability of the methicillin-resistant S. aureus (MRSA) isolates collected from hospitalized patients. As many as 96 % strains had the ability to form biofilm in vitro. The majority...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0175-9
更新日期:2013-01-01 00:00:00
abstract::A long-living artificial tripartite symbiosis involving a green alga (Chlamydomonas), a bacterium (Azotobacter) and a fungus (Alternaria) was established on carbon- and nitrogen-free medium. The basis of the interdependence is the complementation of photosynthetic CO2 assimilation and atmospheric nitrogen fixation. Gr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0067-9
更新日期:2010-07-01 00:00:00
abstract::The green alga Scenedesmus intermedius was grown in synchronous culture under manganese or calcium deficiency for six successive generations. The growth rate, pigment and protein contents gradually decreased in comparison with the control. In Mn-deficient cells, the rate of oxygen evolution was sharply decreased. This...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817561
更新日期:2000-01-01 00:00:00
abstract::The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02815697
更新日期:1996-01-01 00:00:00
abstract::Proteinase production by Lactobacillus murinus was influenced by temperature, glucose concentration, initial pH and nitrogen sources. Maximum proteinase production occurred at 45 degrees C, pH 6.6 and with 0.5% (W/V) glucose. Tryptone, peptone and gelatin inhibited it. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877263
更新日期:1987-01-01 00:00:00
abstract::Adhesion of group B streptococci to human epithelial vaginal and buccal cells proceeded in three phases which differed qualitatively. Maximum adhesion took place within 10 min of interaction, during the second phase (10-15 min), the percentage of adherent cells decreased significantly (P less than 0.05) whereas during...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884088
更新日期:1983-01-01 00:00:00
abstract::Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926454
更新日期:1979-01-01 00:00:00
abstract::Excessive use of antibiotics contributes to the selection of resistant bacteria and intestinal colonization with multiresistant pathogens poses a risk factor for subsequent infections. The present study assessed vancomycin-resistant enterococci (VRE) carriage rates in patients admitted to our tertiary care hospital. S...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00751-x
更新日期:2020-06-01 00:00:00
abstract::Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture fro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-020-00807-3
更新日期:2020-12-01 00:00:00
abstract::Microorganisms in the midgut and rectum of the honeybee were enumerated and characterized. Counts of aerobic microorganisms were distinctly lower than counts of anaerobes (10(5)-10(6) viable cells per g of intestinal content vs. 10(8)-10(9) per g). Total numbers of anaerobic microorganisms were almost identical with t...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931394
更新日期:2004-01-01 00:00:00
abstract::A gram-negative bacterium GXGL-4A was originally isolated from maize roots. It displayed nitrogen-fixing (NF) ability under nitrogen-free culture condition, and had a significant promotion effect on cucumber growth in the pot inoculation test. The preliminary physiological and biochemical traits of GXGL-4A were charac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-018-0608-1
更新日期:2018-11-01 00:00:00
abstract::The soil microbial population, determined over a period of 19 months and the elemental chemical composition measured by X-ray fluorescence techniques were followed in two uncultivated salt-affected areas of Greece, namely Lantza and Klidi. The ecological conditions at Lantza remained stable during the period of study ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02877387
更新日期:1983-01-01 00:00:00
abstract::A total of 40 substances were tested for their inhibitory effect on the multiplication of a bacteriophage in a growing culture of Bacillus licheniformis and their influence on bacitracin production. Acriflavine was the only substance which, at a concentration of 3 microgram ml-1, completely suppressed phage multiplica...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876597
更新日期:1978-01-01 00:00:00
abstract::Using the method of two separate UV exposures the increase of UV resistance after various induction fluences in growing and resting Escherichia coli B/r Hcr+ was followed. In resting cells, the optimum induction energy fluence was found to be 30 J/m2. In exponentially growing cells testing of induction has proved to b...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926452
更新日期:1979-01-01 00:00:00
abstract::A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activi...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02885601
更新日期:1977-01-01 00:00:00