Abstract：
:Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/Go alpha- and Gq alpha/Gl 1 alpha-protein subunits correlated with an increase in acid-sensitive forms of these proteins which was recovered in the mitochondrial and/or lysosomal membrane fraction. In contrast to cells from higher organisms glucose-stimulated yeast exhibits an extremely rapid type of the redistribution (internalization). The question remains open as to the functional significance of the internalized forms of the G-proteins as these remain sequestered from the plasma membrane well after glucose has been consumed.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kotyk A,Ihnatovych I,Lapathitis G,Lamash N,Svoboda Pdoi
10.1007/BF02814427keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
391-6issue
5eissn
0015-5632issn
1874-9356journal_volume
46pub_type
杂志文章abstract：:One-hundred patients with candidosis of the skin, mucous membranes and nail plates (confirmed by cultivation) were examined. Topical or systemic antimycotic treatment was successful in 58 patients. After a complete evaluation, 42 patients were found to suffer from factors supporting candidosis--diabetes mellitus (12),...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02815627
更新日期：2001-01-01 00:00:00
abstract：:Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816378
更新日期：1998-01-01 00:00:00
abstract：:Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins of Clostridium tetani, C. botulinum; lethal factors of C. novyi an...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02928083
更新日期：1988-01-01 00:00:00
abstract：:The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltra...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02814386
更新日期：1993-01-01 00:00:00
abstract：:Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli vir...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02817680
更新日期：2002-01-01 00:00:00
abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931412
更新日期：2005-01-01 00:00:00
abstract：:Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green alga Chlamydomonas eugametos (Vo...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02818794
更新日期：2002-01-01 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections, especially in patients with immunodeficiency. It exhibits multiple mechanisms of resistance, including efflux pumps, antibiotic modifying enzymes and limited membrane permeability. The primary reason for the development of novel therap...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0333-3
更新日期：2015-01-01 00:00:00
abstract：:The resistance to killing by free radicals of two mutants ofPhaffia rhodozyma was determined. Mutant 5-7 did not produce astaxanthin but produced beta-carotene, while mutant 3-4 did not produce any carotenoid pigments. The resistance of mutant 5-7 was the same as that of the wild type but mutant 3-4 was rapidly killed...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02816505
更新日期：1998-01-01 00:00:00
abstract：:Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02903720
更新日期：1999-01-01 00:00:00
abstract：:The synthesis of exocellular proteinase decreases with increasing concentration of amino acids in the growth medium. After removal of amino acids the enzyme synthesis is gradually restored to normal values. The presence of inhibitors of transcription (actinomycin D) or translation (chloramphenicol) blocks the restorat...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02875957
更新日期：1984-01-01 00:00:00
abstract：:When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthe...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876446
更新日期：1982-01-01 00:00:00
abstract：:The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth ch...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-013-0269-z
更新日期：2014-01-01 00:00:00
abstract：:The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3-aminopropyl)dodecy...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931448
更新日期：2006-01-01 00:00:00
abstract：:The activities of inorganic pyrophosphatase, thymidine kinase and thymidine phosphorylase were measured in Ter-mutants of E. coli K12 which have a higher or a lower dTTP pool than the parent strain. The levels of inorganic pyrophosphatase and thymidine kinase were changed in the same direction and that of thymidine ph...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02884076
更新日期：1983-01-01 00:00:00
abstract：:The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-018-0610-7
更新日期：2018-11-01 00:00:00
abstract：:Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digest...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/s12223-008-0024-z
更新日期：2008-01-01 00:00:00
abstract：:The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-008-0067-1
更新日期：2008-01-01 00:00:00
abstract：:A phosphate solubilizing fungus, Aspergillus awamori S29 was isolated from rhizoshpere of mungbean. The phosphate solubilizing activity of A. awamori S29 in liquid was 1,110 mg/L for tricalcium phosphate (TCP). The organism was able to solubilize various inorganic forms of phosphate at a wide range of temperatures. Am...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-012-0167-9
更新日期：2012-11-01 00:00:00
abstract：:The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)₄. The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0091-9
更新日期：2010-11-01 00:00:00
abstract：:The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 8201 fluorescence microscopes at various excitations. The strongest AF of hyphae was found in minimal medium with glucose, where the hyphae exhibited green...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-010-0101-y
更新日期：2010-11-01 00:00:00
abstract：:Mathematical expressions were derived for the dilution rate and concentration of algae determining the maximum biomass production in a semicontinuous algal culture. ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02926655
更新日期：1979-01-01 00:00:00
abstract：:Cultivation media from 11 wood-rotting fungi contained alpha-mannosidase and mannanase activity, alpha-Mannosidase was studied in more detail in Phellinus abietis and mannanase was studied more intimately in basidiomycetes Phellinus abietis, Trametes sanguinea and Pholiota aurivella. Suitable cultivation conditions an...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02876996
更新日期：1977-01-01 00:00:00
abstract：:The temperature dependence of fluorescence anisotropy, lifetime and differential tangent of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its polar trimethylammonium derivative (TMA-DPH) were investigated in cytoplasmic membranes of Bacillus subtilis. The fluorescence parameters were compared in the two types of membranes p...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02821406
更新日期：1990-01-01 00:00:00
abstract：:An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intr...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02879494
更新日期：1983-01-01 00:00:00
abstract：:The morphology, host range, structural proteins and serological properties of Streptococcus thermophilus phages isolated from Finnish cheese plants were investigated. The results show that all the nine phages belong morphologically to Ackermann's group B1. The host-phage reactions and plating efficiency justify the di...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02883235
更新日期：1987-01-01 00:00:00
abstract：:Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-020-00831-3
更新日期：2020-10-24 00:00:00
abstract：:A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters analysis (FAME). All ...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/BF02931556
更新日期：2004-01-01 00:00:00
abstract：:A selection of tert-butylhydroperoxide (tBOOH)-tolerant Candida albicans mutants showed increased tolerances to 19 different stress conditions. These mutants are characterized by a constitutively upregulated antioxidative defense system and, therefore, adaptation to oxidative stress may play an important role in gaini...
journal_title：Folia microbiologica
pub_type： 杂志文章
doi：10.1007/s12223-014-0305-7
更新日期：2014-07-01 00:00:00
abstract：:Ovine and caprine scrapie occupies a unique place among animal transmissive spongiform encephalopathies (TSE). It is an object of intensive biomedicinal, ecological and economical studies. Its causative agents are demonstrably associated with the development of TSE in farmed minks, goats and moufflons. Ovine strains o...
journal_title：Folia microbiologica
pub_type： 杂志文章,评审
doi：10.1007/BF02818713
更新日期：2000-01-01 00:00:00
