Abstract:
:Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated release of cell-surface proteins was studied. Data on calcofluor white and zymolyase sensitivities, resistance to killer 1 toxin and determination of exported acid phosphatase and invertase did not provide evidence for alterations in the cell-wall structure that could explain the protein overexport phenotype. The results were obtained in experiments when transcription of mutated gene was discontinued which permits the full expression of the protein overexport phenotype.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Alexieva KI,Venkov PVdoi
10.1007/BF02908954keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
251-4issue
3eissn
0015-5632issn
1874-9356journal_volume
45pub_type
杂志文章abstract::Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818575
更新日期:1998-01-01 00:00:00
abstract::In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-T...
journal_title:Folia microbiologica
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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journal_title:Folia microbiologica
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更新日期:2018-05-01 00:00:00
abstract::Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (abo...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876611
更新日期:1980-01-01 00:00:00
abstract::The activities of inorganic pyrophosphatase, thymidine kinase and thymidine phosphorylase were measured in Ter-mutants of E. coli K12 which have a higher or a lower dTTP pool than the parent strain. The levels of inorganic pyrophosphatase and thymidine kinase were changed in the same direction and that of thymidine ph...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884076
更新日期:1983-01-01 00:00:00
abstract::Spatial distribution of ectomycorrhizae-associated basidiomycetes was determined in oakbirch forest using terminal restriction fragment length polymorphism (T-RFLP) analysis. The data were correlated with actual soil humidity, pH, electric conductivity of the soil extract, absorbance A(465) and A(665) of water and alk...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0076-8
更新日期:2010-09-01 00:00:00
abstract::Several amino acids affected the amount of bacitracin produced by Bacillus licheniformis ATCC 10716 and the high-yielding mutant B. licheniformis AL. The possibility of an amino acid control mechanism for bacitracin biosynthesis is discussed. The two strains showed a different response to the addition of some amino ac...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02926454
更新日期:1979-01-01 00:00:00
abstract::Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814202
更新日期:1995-01-01 00:00:00
abstract::Lengths of permuted regions in the P22 and L phage genomes were estimated from the relative yields of DNA in many electrophoretic bands obtained using several restriction endonucleases. It was found that 3.6 kb (8.7%) of P22-DNA and 7.2 kb (17.8%) of L-DNA were circularly permuted. In both phages the sequential packag...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02933145
更新日期:1992-01-01 00:00:00
abstract::The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 8201 fluorescence microscopes at various excitations. The strongest AF of hyphae was found in minimal medium with glucose, where the hyphae exhibited green...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0101-y
更新日期:2010-11-01 00:00:00
abstract::As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02898642
更新日期:1997-01-01 00:00:00
abstract::Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli vir...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817680
更新日期:2002-01-01 00:00:00
abstract::Nalidixic acid was used for describing more accurately the terminal replication region of the Mycobacterium phlei chromosome. Cell division in synchronized cultures was not sensitive to this acid any more between 185-190 min, i.e. about 10 min after replication of the ser gene, the last of 24 genes of the replication ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927251
更新日期:1981-01-01 00:00:00
abstract::Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0036-3
更新日期:2009-01-01 00:00:00
abstract::Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931045
更新日期:2004-01-01 00:00:00
abstract::Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by 'polyphenols' and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine....
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0075-9
更新日期:2009-11-01 00:00:00
abstract::A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927364
更新日期:1981-01-01 00:00:00
abstract::Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and degranulation of IgE-sensiti...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02923516
更新日期:1985-01-01 00:00:00
abstract::Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02887577
更新日期:1987-01-01 00:00:00
abstract::Vinasse, a recalcitrant waste of the ethanol industry was employed for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloarcula marismortui in shake flasks. The PHA was recovered by osmotic lysis of the cells and subsequent purification by sodium hypochlorite and organic solvents. ...
journal_title:Folia microbiologica
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doi:10.1007/s12223-011-0092-3
更新日期:2012-01-01 00:00:00
abstract::Structural and functional properties of prokaryotic DNA methyltransferases are summarized. The different aspects of the role of DNA methylation which influences DNA-protein interaction in restriction and modification of DNA and in mismatch repair, DNA replication and gene expression are discussed. ...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02815658
更新日期:1992-01-01 00:00:00
abstract::The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02826557
更新日期:1997-01-01 00:00:00
abstract::The production of bacterial enzymes in batch fermentations is compared with results obtained in continuous culture. When studying the production of alpha-amylase in Bacillus subtilis it was found that instability of the enzyme synthesis was due to nonhomogeneity of the population rather than to "the culture's history"...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02883137
更新日期:1982-01-01 00:00:00
abstract::The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-017-0563-2
更新日期:2018-05-01 00:00:00
abstract::Oxidizing and assimilating ability of the yeast Candida utilis 49 was tested with 21 different low-boiling organic compounds which come as components of raw synthetic ethanol. The highest yields of yeast dry weight were obtained with ethanol (72.0%), propanol (48.2%), ethyl acetate (43.4%) and acetic acid (34.2%). To ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02876965
更新日期:1976-01-01 00:00:00
abstract::Antibody response to major porin proteins of S. Typhi (OmpC and OmpF) was evaluated in sera of typhoid patients (culture positive, n = 28; Widal positive, n = 16). Sera from fever patients (n = 6) having etiology other than Salmonella, and normal healthy human controls (n = 18) were also included. No significant diffe...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0086-6
更新日期:2010-09-01 00:00:00
abstract::Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931594
更新日期:2006-01-01 00:00:00
abstract::Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of th...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931380
更新日期:2003-01-01 00:00:00
abstract::Genetic mapping of the chromosome of the bacteriophage PS8 of Agrobacterium tumefaciens using temperature sensitive (ts) mutants of the bacteriophage is described. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02927304
更新日期:1979-01-01 00:00:00
abstract::Some Bacillus subtilis mutants with different levels of homoserine dehydrogenase were described. Strains that do not accumulate methionine have a high homoserine dehydrogenase activity. Low activity was detected in mutants where cell growth was completely inhibited by 0.7 mmol/L methionine. A low concentration of dime...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884064
更新日期:1991-01-01 00:00:00