Abstract:
:Murine herpesvirus 68 (MHV-68) is a natural pathogen that infects murid rodents, which serves as hosts for Ixodes ricinus ticks. For the first time, MHV-68 was detected in immature I. ricinus ticks feeding on Lacerta viridis lizards trapped in Slovakia, which supports the idea that ticks can acquire the virus from feeding on infected hosts. The recent discovery of MHV-68 infection and MHV-68 M3 gene transcripts in Dermacentor reticulatus ticks collected in Slovakia also supports this suggestion. Here, for the first time, we report MHV-68 infection, which was detected by nested PCR, in I. ricinus adults collected from the vegetation, and the viral load in infected ticks was determined by quantitative PCR. The viral incidence in ticks was 38.1% (21/55), and the viral load varied from 1.5 × 103 to 2.85 × 104 genome copies per tick. These results suggest that the I. ricinus ticks became infected with MHV-68 from biting infected rodents; thus, I. ricinus ticks may play a role in the spread of this virus in nature.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Kúdelová M,Jánošová M,Belvončíková Pdoi
10.1007/s12223-018-0586-3subject
Has Abstractpub_date
2018-07-01 00:00:00pages
511-515issue
4eissn
0015-5632issn
1874-9356pii
10.1007/s12223-018-0586-3journal_volume
63pub_type
杂志文章abstract::Glycerol-based medium (BM) with and without the addition of 1 g/L ascorbic acid (Asc) and/or 100 mg/L (+/-)-catechin (Cat) was tested for the storage of three commercial wine yeasts at -20 degrees C. The medium supplemented with Asc was also used to store 706 strains to verify the maintenance of the liquid state. A de...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0036-3
更新日期:2009-01-01 00:00:00
abstract::Vancomycin is often used in orthopedic surgery as a local prophylaxis of bacterial infection. The aim of this work was to compare the release of vancomycin and its biologically inactive crystalline degradation products (CDP-1) during in vitro experiments from different types of local antibiotic delivery systems (bone ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-019-00752-w
更新日期:2020-06-01 00:00:00
abstract::The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02816234
更新日期:1999-01-01 00:00:00
abstract::Inhibition activity of the alkaloid (-)-corypalmine on spore germination of plant pathogenic and saprophytic fungi (Alternaria solani, A brassicicola, A. brassicae, A. melongenae, Curvularia pallescens, C. lunata, C. maculans, Curvularisa sp., Colletotrichum sp., Helminthosporium speciferum, H. frumentacei, H. pennise...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817654
更新日期:2002-01-01 00:00:00
abstract::The efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (MIC) and time-kill curve analysis in Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and Citrobacter braakii; MIC was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/L in...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-009-0054-1
更新日期:2009-09-01 00:00:00
abstract::We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium sm...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0029-2
更新日期:2010-05-01 00:00:00
abstract::Altered cell signaling is the molecular basis for cell proliferation occurring in association with several gamma herpesvirus infections. Three gamma herpesviruses, namely EBV/HHV-4, KSHV/HHV-8 and the MHV-68 (and/or MHV-72) and their unusual cell-pirated gene products are discussed in this respect. The EBV, KSHV as we...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931360
更新日期:2003-01-01 00:00:00
abstract::The kinetics of extracellular neutral proteinase synthesis by an isogenic stringent (IS58) and a relaxed (IS56) strain of B. subtilis were compared. The specific enzyme formation rate by the stringent strain was higher than that of the relaxed one. Norvaline addition (1 mg/mL) induced the formation of pppGpp and ppGpp...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02883234
更新日期:1987-01-01 00:00:00
abstract::The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)₄. The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0091-9
更新日期:2010-11-01 00:00:00
abstract::Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simp...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0165-y
更新日期:2012-11-01 00:00:00
abstract::The addition of a nitrogen (nitrate) and carbon sources (acetate, citrate and fructose) and phosphate deficiency (nitrate medium deficient in phosphate) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures of this organism in these nutrients did not differentiate akinetes. ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02817617
更新日期:2000-01-01 00:00:00
abstract::As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases Mycoplasma mycoides and Acholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814471
更新日期:1989-01-01 00:00:00
abstract::We have investigated the expression of cyclins, cyclin dependent kinases (CDK), and CDK inhibitors (CKI) at the mRNA level in a panel of small-cell lung cancer (SCLC) cell lines in vitro and in vivo as xenografts in nude mice. The results showed that the cell lines expressed varying amounts of most cyclin and CDK's bu...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02820804
更新日期:1998-01-01 00:00:00
abstract::For the first time, it was reported in France a cluster of autochthonous severe community-acquired (CA) infections due to the USA300 methicillin-resistant Staphylococcus aureus (MRSA) clone. The three cases belonged to the same family without any identified clue of abroad importation pathway. The domestic spread of US...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-014-0322-6
更新日期:2014-11-01 00:00:00
abstract::Candidiases, infections caused by germination forms of the Candida fungus, represent a heterogeneous group of diseases from systemic infection, through mucocutaneous form, to vulvovaginal form. Although caused by one organism, each form is controlled by distinct host immune mechanisms. Phagocytosis by polymorphonuclea...
journal_title:Folia microbiologica
pub_type: 杂志文章,评审
doi:10.1007/BF02931313
更新日期:2007-01-01 00:00:00
abstract::We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02814307
更新日期:1994-01-01 00:00:00
abstract::Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-012-0124-7
更新日期:2012-07-01 00:00:00
abstract::The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laborato...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-008-0067-1
更新日期:2008-01-01 00:00:00
abstract::Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818575
更新日期:1998-01-01 00:00:00
abstract::Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded dur...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0004-y
更新日期:2010-01-01 00:00:00
abstract::Hydroxy, n-saturated, branched, dioic, and unsaturated fatty acids in six freshwater wild cyanobacteria (Chroococcus minutus, Lyngbya ceylanica, Merismopedia glauca, Nodularia sphaerocarpa, Nostoc linckia, and Synechococcus aeruginosus) collected from different lakes and springs of Israel have been identified by gas c...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931279
更新日期:2003-01-01 00:00:00
abstract::Submerged cultures of the low-production strain of Tolypocladium inflatum DSM 63544 formed a mixture of cyclosporins (CS) consisting of CS-A, CS-B, "CS-3" and "CS-4". Glucose, sucrose and maltose were highly favored for biomass production but provided a different physiological state necessary for CS biosynthesis. Not ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02884035
更新日期:1991-01-01 00:00:00
abstract::Stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. The aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (MAD) and thermophilic anaerobic digestion (TAD), on bacterial communities in sludge,...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-015-0396-9
更新日期:2015-11-01 00:00:00
abstract::Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931454
更新日期:2005-01-01 00:00:00
abstract::Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02816378
更新日期:1998-01-01 00:00:00
abstract::beta-Glucosidase from the fungus Thermoascus aurantiacus grown on semi-solid fermentation medium (using ground corncob as substrate) was partially purified in 5 steps--ultrafiltration, ethanol precipitation, gel filtration and 2 anion exchange chromatography runs, and characterized. After the first anion exchange chro...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02818672
更新日期:2002-01-01 00:00:00
abstract::The plasma membrane and mitochondria of bottom fermenting brewer's yeast obtained as a by-product of industrial beer production were isolated and the lipid fraction was analyzed. The phospholipid content accounted for 78 mg/g protein in the plasma membrane and 59 mg/g protein in the mitochondria. Major phospholipids i...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02931290
更新日期:2005-01-01 00:00:00
abstract::Spatial distribution of ectomycorrhizae-associated basidiomycetes was determined in oakbirch forest using terminal restriction fragment length polymorphism (T-RFLP) analysis. The data were correlated with actual soil humidity, pH, electric conductivity of the soil extract, absorbance A(465) and A(665) of water and alk...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/s12223-010-0076-8
更新日期:2010-09-01 00:00:00
abstract::The vacA and cagA genotypes of Helicobacter pylori exhibited distinct geographic distribution and correlation with severity of disease. In the above genotypes (obtained from 150 H. pylori-positive patients--139 with gastritis, 10 with ulcer and 1 patient with gastric cancer) combinations vacA s1/m1 and s2/m2 were dete...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02932113
更新日期:2007-01-01 00:00:00
abstract::Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated rel...
journal_title:Folia microbiologica
pub_type: 杂志文章
doi:10.1007/BF02908954
更新日期:2000-01-01 00:00:00