Abstract:
:A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria.
journal_name
Folia Microbiol (Praha)journal_title
Folia microbiologicaauthors
Mrázek J,Koppová I,Kopecný J,Simůnek J,Fliegerová Kdoi
10.1007/s12223-010-0057-ysubject
Has Abstractpub_date
2010-07-01 00:00:00pages
352-8issue
4eissn
0015-5632issn
1874-9356journal_volume
55pub_type
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