454-Pyrosequencing Reveals Microbial Community Structure and Composition in a Mesophilic UAFB System Treating PTA Wastewater.

Abstract:

:To well understand the community structure and composition of mesophilic microorganisms in anaerobic system fed with PTA wastewater, an up-flow anaerobic fixed bed reactor was continuously run at 33 and 37 °C for 75 and 60 days, respectively. Both fluorescence in situ hybridization analysis and 454-pyrosequencing were applied to investigate the microbial distinction within mesophilic ranges. A preferable performance was achieved at 37 than 33 °C. The taxonomic complexities of two samples were further compared at phylum, class, and genus levels. Notably, microbial diversity differed a lot and the change of populations was observed mainly in the shared OTUs. Genus level analysis showed that when temperature was increased to 37 °C, the abundance of Thauera and Hydrogenophaga (β-Proteobacteria) decreased by 93.75 and 61.47 %, respectively, whereas that of Syntrophorhabdus (δ-Proteobacteria) increased from 4.93 to 16.01 %. Furthermore, the dominant archaeal Methanobacterium at both temperatures indicated the prevailing contribution of hydrogenotrophic methanogens in mesophilic anaerobic system.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Ma KL,Li XK,Wang K,Zhou HX,Meng LW,Zhang J

doi

10.1007/s00284-015-0884-9

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

551-8

issue

5

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-015-0884-9

journal_volume

71

pub_type

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