Abstract:
:To well understand the community structure and composition of mesophilic microorganisms in anaerobic system fed with PTA wastewater, an up-flow anaerobic fixed bed reactor was continuously run at 33 and 37 °C for 75 and 60 days, respectively. Both fluorescence in situ hybridization analysis and 454-pyrosequencing were applied to investigate the microbial distinction within mesophilic ranges. A preferable performance was achieved at 37 than 33 °C. The taxonomic complexities of two samples were further compared at phylum, class, and genus levels. Notably, microbial diversity differed a lot and the change of populations was observed mainly in the shared OTUs. Genus level analysis showed that when temperature was increased to 37 °C, the abundance of Thauera and Hydrogenophaga (β-Proteobacteria) decreased by 93.75 and 61.47 %, respectively, whereas that of Syntrophorhabdus (δ-Proteobacteria) increased from 4.93 to 16.01 %. Furthermore, the dominant archaeal Methanobacterium at both temperatures indicated the prevailing contribution of hydrogenotrophic methanogens in mesophilic anaerobic system.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Ma KL,Li XK,Wang K,Zhou HX,Meng LW,Zhang Jdoi
10.1007/s00284-015-0884-9subject
Has Abstractpub_date
2015-11-01 00:00:00pages
551-8issue
5eissn
0343-8651issn
1432-0991pii
10.1007/s00284-015-0884-9journal_volume
71pub_type
杂志文章abstract::Streptococcus bovis is commonly present in the rumen, but strains of S. bovis have also occasionally been isolated from human blood or fecal samples. Studies were undertaken with 16s rRNA gene sequences and DNA hybridizations to define the genetic relationships between these two groups of strains. Ruminal strains were...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00314583
更新日期:1995-11-01 00:00:00
abstract::By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9476-x
更新日期:2009-11-01 00:00:00
abstract::Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not pr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3976-2
更新日期:2003-09-01 00:00:00
abstract::Lactobacilli are members of the normal mucosal microflora of most animals. Many isolates of Lactobacillus spp. are adherent to epithelial cells. In this study, using Lactobacillus acidophilus and L. agilis, we detected adherence in a pattern that suggested that the bacteria were binding to extracellular matrix protein...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010107
更新日期:2000-08-01 00:00:00
abstract::The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated f...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4358-8
更新日期:2004-10-01 00:00:00
abstract::Mycoplasma penetrans invades HeLa cells and survives within them for prolonged periods of time. The intracellular distribution of M. penetrans within HeLa cells was studied utilizing the acidotropic dye LysoTracker (green), which permeates cell membranes and upon protonation remains trapped in acidic compartments. The...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4298-3
更新日期:2004-08-01 00:00:00
abstract::Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted levansucrase (LsdA). A levanase-encoding gene ( lsdB), starting 51 bp downstream of the lsdA gene, was cloned from strain SRT4. The lsdB gene (1605 bp) encodes a protein (calculated molecular mass 58.4 kDa) containing a putative 36-amino-acid s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0044-2
更新日期:2002-07-01 00:00:00
abstract::Pseudomonas aeruginosa is a serious pathogen involved in nosocomial infections. Its pathogenicity is owed to rich production of virulence factors (VIFs) regulated by several complex hierarchical signal systems depending on environmental conditions, medium composition, and the presence of certain active compounds in it...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0441-7
更新日期:2007-04-01 00:00:00
abstract::Bacterial fish pathogens are pervasive in aquaculture. Control of bacterial fish pathogen is a difficult task among aquaculture practitioners. A large number of antibiotics are used for the control of prevalent bacterial pathogens in aquaculture. This may lead to drug resistance among pathogens and further treatment w...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1506-0
更新日期:2018-09-01 00:00:00
abstract::A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01577445
更新日期:1994-11-01 00:00:00
abstract::Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cy...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0383-9
更新日期:2013-10-01 00:00:00
abstract::Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900082
更新日期:1996-08-01 00:00:00
abstract::This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9098-8
更新日期:2008-09-01 00:00:00
abstract::All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02150-9
更新日期:2020-11-01 00:00:00
abstract::Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1208-z
更新日期:2017-04-01 00:00:00
abstract::Plasmids play a central role in engineering recombinant bacteria because they are the primary vehicles used to manipulate targeted sequences. In some cases, bacteria of interest are poorly provided with suitable tools for these molecular or genetic manipulations. In this context, we constructed from two shuttle clonin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0358-1
更新日期:2007-05-01 00:00:00
abstract::Trioxacarcin A is a polyoxygenated, structurally complex antibiotic produced by Streptomyces spp., which possesses high anti-bacterial, anti-malaria, and anti-tumor activities. The trioxacarcin biosynthetic pathway involves type II polyketide synthases (PKSs) with L-isoleucine as a unique starter unit, as well as many...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0868-9
更新日期:2015-10-01 00:00:00
abstract::Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01922-7
更新日期:2020-07-01 00:00:00
abstract::Erwinia amylovora causes fire blight of apple, pear, and other members of the Rosaceae family. The enzyme LuxS catalyzes the last step in the production of autoinducer-2 (AI-2), a molecule implicated with quorum sensing in many bacterial species. It is now well recognized that LuxS also plays a central role in sulfur ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9256-z
更新日期:2009-01-01 00:00:00
abstract::The aim of this study is to investigate the combination of cefepime and sulbactam. Sulbactam, when administered , will effectively inhibit all Extended-spectrum beta lactamases (ESBLs) of the microorganism, while cefepime will inhibit the growth of the resistant microorganisms since it will not be hydrolyzed by OXA-48...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02094-0
更新日期:2020-09-01 00:00:00
abstract::Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus pr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3789-3
更新日期:2003-04-01 00:00:00
abstract::Nine phage isolates infectious for Vibrio vulnificus and falling into four morphological groups were isolated from estuarine waters collected in Louisiana. Of the 60 V. vulnificus strains tested, 87% were susceptible to one or more of the isolates. With the exception of V. fluvialis, Vibrio species other than vulnific...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00369859
更新日期:1995-06-01 00:00:00
abstract::In past years, the significance of microbial resistance to biocides has increased. Twenty biocide-resistant bacterial strains were isolated from dental-unit water line biofilm. All strains resisted high biocide concentrations (up to 100 microg ml(-1)): sodium dodecyl sulphate, hydrogen peroxide, sodium hypochlorite, p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9200-2
更新日期:2008-10-01 00:00:00
abstract::Potassium tellurite (K2TeO3) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K2TeO3 resistance in STEC is attributable to the ter gene cluster with terD an indicator of the c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1444-x
更新日期:2018-06-01 00:00:00
abstract::A rhizobacterium with high antifungal activity was isolated from a potato field at Inneruulalik, South Greenland. Phylogenetic analysis based on multi locus sequence typing showed that the bacterium was affiliated with strains of Pseudomonas fluorescens. The bacterium, denoted as Pseudomonas fluorescens In5, inhibited...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9846-4
更新日期:2011-04-01 00:00:00
abstract::Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1225-y
更新日期:2017-05-01 00:00:00
abstract::The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment sam...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0056-5
更新日期:2012-03-01 00:00:00
abstract::The citric acid-producing fungus Aspergillus niger WU-2223L possesses a cyanide-insensitive respiratory pathway catalyzed by alternative oxidase. The regulation of the alternative oxidase under the conditions of citric acid production was determined from the transcription level of the alternative oxidase gene (aox1). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9369-z
更新日期:2009-04-01 00:00:00
abstract::A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9469-9
更新日期:2009-11-01 00:00:00
abstract::Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli....
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9537-1
更新日期:2010-04-01 00:00:00