Abstract:
:This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac (line ISOC) was used to screen Cry1Ac-expressing strains of B. thuringiensis for additional toxins with activity against H. armigera. Using this approach, a number of Cry1Ac-producing strains with significant toxicity toward Cry1Ac-resistant H. armigera were identified. When the insecticidal protein complement of one of these strains, C81, was examined in detail, a novel cry2 gene (cry2Af1) was detected.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Beard CE,Court L,Mourant RG,James B,Van Rie J,Masson L,Akhurst RJdoi
10.1007/s00284-008-9098-8subject
Has Abstractpub_date
2008-09-01 00:00:00pages
175-80issue
3eissn
0343-8651issn
1432-0991journal_volume
57pub_type
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