Use of a Cry1Ac-resistant line of Helicoverpa armigera (Lepidoptera: Noctuidae) to detect novel insecticidal toxin genes in Bacillus thuringiensis.

Abstract:

:This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac (line ISOC) was used to screen Cry1Ac-expressing strains of B. thuringiensis for additional toxins with activity against H. armigera. Using this approach, a number of Cry1Ac-producing strains with significant toxicity toward Cry1Ac-resistant H. armigera were identified. When the insecticidal protein complement of one of these strains, C81, was examined in detail, a novel cry2 gene (cry2Af1) was detected.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Beard CE,Court L,Mourant RG,James B,Van Rie J,Masson L,Akhurst RJ

doi

10.1007/s00284-008-9098-8

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

175-80

issue

3

eissn

0343-8651

issn

1432-0991

journal_volume

57

pub_type

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