PCR methods for identification and specific detection of probiotic lactic acid bacteria.

abstract：:Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...

journal_title：Current microbiology

authors： Xu YY,Huang CJ,Xu L,Jiang XW,Xu XW,Xu XW

更新日期：2020-10-01 00:00:00

abstract：:A novel leucine aminopeptidase was purified from a Bacillus thuringiensis israelensis (Bti) culture. The purification stages included heating the concentrated supernatant to 65 degrees C for 90 min, anion-exchange chromatography by DEAE cellulose, and hydrophobic chromatography by phenyl Sepharose. The specific activi...

journal_title：Current microbiology

authors： Cahan R,Hetzroni E,Nisnevitch M,Nitzan Y

更新日期：2007-11-01 00:00:00

abstract：:This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was exa...

journal_title：Current microbiology

authors： Bru-Adan V,Wéry N,Moletta-Denat M,Boiron P,Delgènes JP,Godon JJ

更新日期：2009-09-01 00:00:00

abstract：:Findings show 21 fungal isolates belonging to eight genera recovered from Egyptian soils that have the potential to attack L-methionine under submerged conditions. Aspergillus flavipes had the most methioninolytic activity, giving the highest yield of L-methioninase (10.78 U/mg protein), rate of methionine uptake (93....

journal_title：Current microbiology

authors： Khalaf SA,El-Sayed AS

更新日期：2009-03-01 00:00:00

abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...

journal_title：Current microbiology

authors： Liu Z,Xie Y,Zhang X,Hu X,Li Y,Ding X,Xia L,Hu S

更新日期：2016-04-01 00:00:00

abstract：:Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8(T) under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemastertrade mark 2D Platinum software. The molecular masses of the majorit...

journal_title：Current microbiology

authors： Feng DQ,Yang LF,Lu WD,Yang SS

更新日期：2007-01-01 00:00:00

abstract：:Corynebacterium diphtheriae strains expressed variation in hydrophobic characteristics dependent on the method used. Results of single assays are not a reliable representation of C. diphtheriae hydrophobicity. All 12 strains adhered to polystyrene surfaces; three showed spontaneous aggregation (SA) in Trypticase Soy B...

journal_title：Current microbiology

authors： Mattos-Guaraldi AL,Formiga LC,Andrade AF

更新日期：1999-01-01 00:00:00

abstract：:The goal of this work was the investigation of correlation between some peculiarities of membrane fatty acids composition, biofilm formation, and motility of dam and/or seqA mutants in Salmonella typhimurium bacterial cells and UV-C radiations. The exposure changed the fatty acids composition of dam and seqA/dam strai...

journal_title：Current microbiology

authors： Abdelwaheb C,Lobna M,Bouchra BA,Selma K,Ahmed L

更新日期：2015-10-01 00:00:00

abstract：:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized a...

journal_title：Current microbiology

authors： Karunakaran T,Gunasekaran P

更新日期：1992-11-01 00:00:00

abstract：:The [2Fe-2S] cluster containing ferredoxin has attracted much attention in recent years. Genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins and functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. The gene of ferred...

journal_title：Current microbiology

authors： Zeng J,Huang X,Liu Y,Liu J,Qiu G

更新日期：2007-12-01 00:00:00

abstract：:Biofilm-forming ability is well established as an important virulence factor. However, there are no studies available regarding biofilm formation of Salmonella Typhimurium 1,4,[5],12:i:-, the new pandemic serovar in Europe. To address this problem, biofilm expression by Salmonella 1,4,[5],12:i:- was evaluated using 13...

journal_title：Current microbiology

authors： Seixas R,Machado J,Bernardo F,Vilela C,Oliveira M

更新日期：2014-05-01 00:00:00

abstract：:Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...

journal_title：Current microbiology

authors： Guerlava P,Izac V,Tholozan JL

更新日期：1998-03-01 00:00:00

abstract：:On the basis of the pulsed field gel electrophoresis(PFGE) technique, molecular karyotypes in Amoeboaphelidiumprotococcarum (the endotrophic parasite of chlorophycean algae, whichcombines protozoon- and fungus-type characters) were determined. Molecularkaryotypes in the strains X1, X5, and X31, which differ in the hos...

journal_title：Current microbiology

authors： Pinevich A,Gromov B,Mamkaeva K,Nasonova E

更新日期：1997-02-01 00:00:00

abstract：:Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used...

journal_title：Current microbiology

authors： Williams PD,Eichstadt SL,Kokjohn TA,Martin EL

更新日期：2007-07-01 00:00:00

abstract：:Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...

journal_title：Current microbiology

authors： Ennahar S,Deschamps N,Richard J

更新日期：2000-07-01 00:00:00

abstract：:Pandemic influenza A (H1N1) 2009 virus was first detected in India in May 2009 which subsequently became endemic in many parts of the country. Influenza A viruses have the ability to evade the immune response through its ability of antigenic variations. The study aims to characterize influenza A (H1N1) pdm 09 viruses ...

journal_title：Current microbiology

authors： Gohil D,Kothari S,Shinde P,Meharunkar R,Warke R,Chowdhary A,Deshmukh R

更新日期：2017-08-01 00:00:00

abstract：:Rep22 is the replication region of the lactococcal theta replicating pUCL22 plasmid. The copy number of Rep22-based plasmids in Lactococcus was determined by using a chromosomal DNA fragment from Lactococcus lactis subsp. lactis MMS368 as reference. Segregational behavior appeared to be linked to copy number and there...

journal_title：Current microbiology

authors： Frère J,Herreman C,Boutibonnes P,Novel M,Novel G

更新日期：1995-01-01 00:00:00

abstract：:A novel actinomycete, designated strain LAM7112T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20-45 °C (optimum: 35 °C), pH 5.0-10.0 (optimum: 7.0) and in the presence of 0-10.0% (optimum: 3.0%) (w/v) NaCl. The ...

journal_title：Current microbiology

authors： Ma Q,Zhang Q,Jiang X,Kong D,Han X,Xue H,Zhou Y,Zhang Y,Zhang W,Ruan Z

更新日期：2020-11-01 00:00:00

abstract：:The goal of this study was to evaluate how two new hydrolysates from poultry by-products act on ten lactobacilli growth kinetics when supplemented to the growth medium. These effects were compared with ones induced by two most common commercial hydrolysates, i.e., tryptone and peptone. Growth medium, supplemented with...

journal_title：Current microbiology

authors： Meli F,Lazzi C,Neviani E,Gatti M

更新日期：2014-01-01 00:00:00

abstract：:Two strains of Rhizobia isolated from nodules of Vicia faba var. major and one strain isolated from nodules of Cicer arietinum L. were characterized for salt resistance. The presence of 1 mM glycine betaine or choline in a minimal medium with added NaCl had a beneficial role on the growth of the three strains. Both mo...

journal_title：Current microbiology

authors： Brhada F,Poggi MC,Le Rudulier D

更新日期：1997-03-01 00:00:00

abstract：:The connection between soil and microbes plays a critical role in soil health and quality and can be elastic with the application of soil amendments and/or crop rotations. Inappropriate management of soil and application of impermissible levels of fertilizers ruptures the overriding connection between the soil and mic...

journal_title：Current microbiology

authors： Nagaraju Y,Gundappagol RC,Mahadevaswamy

更新日期：2020-09-01 00:00:00

abstract：:The complex gut microbiota plays a key role in host metabolism and health. However, the core microbial communities in the different aged Bactrian camels remain totally unclear. We used high-throughput 16S rRNA gene sequencing to examine the temporal variability of the fecal microbiota in Bactrian camels. At 2 months o...

journal_title：Current microbiology

authors： He J,Hai L,Orgoldol K,Yi L,Ming L,Guo F,Li G,Ji R

更新日期：2019-07-01 00:00:00

abstract：:Bacteria use quorum sensing signalling in various functions, e.g. while forming biofilms, and inhibition of this signalling could be one way to control biofilm formation. The aim of this study was to evaluate the production of signalling molecules and its correlation with the biofilm formation capability of bacteria i...

journal_title：Current microbiology

authors： Priha O,Virkajärvi V,Juvonen R,Puupponen-Pimiä R,Nohynek L,Alakurtti S,Pirttimaa M,Storgårds E

更新日期：2014-11-01 00:00:00

abstract：:Cronobacter sakazakii is now recognized as an opportunistic pathogen and has been implicated in rare but severe cases of necrotizing enterocolitis, meningitis, and sepsis in neonates. The first step in bacterial pathogenesis requires that the organism adheres to host cells surfaces; therefore, agents that inhibit adhe...

journal_title：Current microbiology

authors： Quintero-Villegas MI,Wittke A,Hutkins R

更新日期：2014-10-01 00:00:00

abstract：:A gene encoding the rice (Oryza sativa L.) 90-kDa heat shock protein (OsHsp90) was introduced into Escherichia coli using the pGEX-6p-3 expression vector with a glutathione-S-transferase (GST) tag to analyze the possible function of this protein under heat stress for the first time. We compared the survivability of E....

journal_title：Current microbiology

authors： Liu D,Lu Z,Mao Z,Liu S

更新日期：2009-02-01 00:00:00

abstract：:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...

journal_title：Current microbiology

authors： Pooler MR,Hartung JS

更新日期：1995-12-01 00:00:00

abstract：:The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...

journal_title：Current microbiology

authors： Santos-Buelga JA,Collins MD,East AK

更新日期：1998-11-01 00:00:00

abstract：:Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacter...

journal_title：Current microbiology

authors： Kasana RC,Sharma UK,Sharma N,Sinha AK

更新日期：2007-06-01 00:00:00

abstract：:Probiotics are well known for their wide range of beneficial activities. However, recent use of probiotic Bifidobacterium, Enterococcus, and Lactobacillus spp. has been plagued by certain disadvantages such as complex growth requirements, high maintenance cost, susceptibility to the gastrointestinal environment, patho...

journal_title：Current microbiology

authors： Priyodip P,Balaji S

更新日期：2019-04-01 00:00:00

abstract：:The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...

journal_title：Current microbiology

authors： Reddy YC,Venkateswerlu G

更新日期：2002-12-01 00:00:00