Abstract：
:Two strains of Rhizobia isolated from nodules of Vicia faba var. major and one strain isolated from nodules of Cicer arietinum L. were characterized for salt resistance. The presence of 1 mM glycine betaine or choline in a minimal medium with added NaCl had a beneficial role on the growth of the three strains. Both molecules were found to be taken up by cells obtained at low osmolarity, and whereas glycine betaine uptake activity was stimulated significantly in cells grown in the presence of 0.15 M NaCl, choline uptake activity was strongly inhibited by salt in all tested strains. However, in cells grown with exogenous choline,the uptake inhibition exerted by salt was relieved, mainly in the strain isolated from nodules of C. arietinum L. On the basis of kinetics determinations, in control cells as well as in salt-stressed cells, only high-affinity activities were observed for glycine betaine and choline(apparent Kms between 3 and 18 micro;M). Periplasmic proteins that bound glycine betaine or choline were identified. In nondenaturing conditions, these proteins extracted from the various strains showed different electrophoretic mobility with always a less negative entire charge than the analogous proteins from Rhizobium meliloti.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Brhada F,Poggi MC,Le Rudulier Ddoi
10.1007/s002849900163subject
Has Abstractpub_date
1997-03-01 00:00:00pages
167-72issue
3eissn
0343-8651issn
1432-0991journal_volume
34pub_type
杂志文章abstract：:On the basis of the pulsed field gel electrophoresis(PFGE) technique, molecular karyotypes in Amoeboaphelidiumprotococcarum (the endotrophic parasite of chlorophycean algae, whichcombines protozoon- and fungus-type characters) were determined. Molecularkaryotypes in the strains X1, X5, and X31, which differ in the hos...
journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：1997-02-01 00:00:00
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doi：10.1007/s00284-019-01730-8
更新日期：2019-11-01 00:00:00
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journal_title：Current microbiology
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更新日期：2007-09-01 00:00:00
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更新日期：2013-07-01 00:00:00
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journal_title：Current microbiology
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更新日期：2005-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900319
更新日期：1998-06-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：1997-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2006-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00284-015-0858-y
更新日期：2015-10-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-011-9960-y
更新日期：2011-08-01 00:00:00
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更新日期：2017-03-01 00:00:00
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更新日期：1999-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900384
更新日期：1998-11-01 00:00:00
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更新日期：2012-02-01 00:00:00
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更新日期：2008-02-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-019-01689-6
更新日期：2019-07-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-009-9520-x
更新日期：2010-03-01 00:00:00
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doi：10.1007/s00284-020-02034-y
更新日期：2020-09-01 00:00:00
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journal_title：Current microbiology
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更新日期：2014-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9585-6
更新日期：2010-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1372-1
更新日期：2018-03-01 00:00:00
