Abstract：
:The dimorphic yeast Yarrowia lipolytica has become an emerging cell factory for recombinant proteins production. Expression vectors involving LIP2 promoter (pLIP2) have been developed and used successfully. However, the relationship between dimorphic transition (i.e., cell morphology) and pLIP2 regulation is still unclear and must be assessed to improve process robustness. This requests to discriminate the effect of cell morphology from that of effectors, such as pH, that trigger the dimorphic transition. This was performed using gene reporter system based on β-galactosidase activity and DsRed fluorescence, single-cell analysis by flow cytometry, and quantification of gene expression. Our results clearly pointed out that cell morphology has not effect on the regulation of pLIP2. By contrast, pH modification yielded to phenotypic heterogeneity, potentially leading to a lack of robustness of the cell population. Taken altogether, our results demonstrated that, under appropriate environmental conditions (e.g., pH being an important factor), Y. lipolytica could be considered as a robust and reliable host for recombinant protein production.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Sassi H,Delvigne F,Kallel H,Fickers Pdoi
10.1007/s00284-017-1207-0subject
Has Abstractpub_date
2017-03-01 00:00:00pages
413-417issue
3eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1207-0journal_volume
74pub_type
信件abstract：:A novel thermostable phytase gene was cloned from Aspergillus fumigatus WY-2. It was 1459 bp in size and encoded a polypeptide of 465 amino acids. The gene was expressed in Pichia pastoris GS115 as an extracellular enzyme. The expressed enzyme was purified to homogeneity and biochemically characterized. The purified e...
journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：2009-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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journal_title：Current microbiology
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更新日期：2005-01-01 00:00:00
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更新日期：2015-08-01 00:00:00
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更新日期：2008-07-01 00:00:00
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更新日期：2011-03-01 00:00:00
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更新日期：2011-12-01 00:00:00
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journal_title：Current microbiology
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更新日期：2011-05-01 00:00:00
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journal_title：Current microbiology
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更新日期：2007-04-01 00:00:00
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journal_title：Current microbiology
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更新日期：1978-01-01 00:00:00
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journal_title：Current microbiology
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更新日期：2008-08-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/BF00369859
更新日期：1995-06-01 00:00:00
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更新日期：2011-05-01 00:00:00
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更新日期：2014-05-01 00:00:00
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更新日期：2003-09-01 00:00:00
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更新日期：2019-12-01 00:00:00
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更新日期：2018-03-01 00:00:00
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更新日期：2018-05-01 00:00:00
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更新日期：2020-10-01 00:00:00
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更新日期：1980-07-01 00:00:00
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journal_title：Current microbiology
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journal_title：Current microbiology
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