Abstract：
:The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated four distinct phylotypes. The terpenoid metabolite profiles of each phylotype, determined by liquid chromatography mass spectrometry (LCMS) and nuclear magnetic resonance (NMR) analyses, indicated that diterpenoid production was restricted to phylotype A, which included the type specimen S. grandis Gross, and the sole member of the closely related phylotype B. The discovery of two new neoverrucosane diterpenoids produced by phylotype B has also been documented.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Mincer TJ,Spyere A,Jensen PR,Fenical Wdoi
10.1007/s00284-004-4358-8keywords:
subject
Has Abstractpub_date
2004-10-01 00:00:00pages
300-7issue
4eissn
0343-8651issn
1432-0991journal_volume
49pub_type
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