Abstract：
:A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase chain reaction (PCR) revealed that INA-02 contained the cryIA(a) and cryV genes. Since no Spodoptera activity was observed with B. thuringiensis sotto, which contained only cryIA(a), insecticidal activity of the protein encoded by the cryV gene was investigated with several insect species including S. litura. For bioassay, the cryV gene was highly expressed in an acrystalliferous B. thuringiensis strain, BT51. The CryV protein from BT51 was assayed against larvae of three lepidopteran species, Bombyx mori, S. litura, and Plutella xylostella. The protein was highly active against S. litura and P. xylostella, suggestive that the protein contributes to the unique activity of INA-02.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Sasaki J,Asano S,Iizuka T,Bando H,Lay BW,Hastowo S,Powell GK,Yamamoto Tdoi
10.1007/s002849900035subject
Has Abstractpub_date
1996-04-01 00:00:00pages
195-200issue
4eissn
0343-8651issn
1432-0991journal_volume
32pub_type
杂志文章abstract：:Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted levansucrase (LsdA). A levanase-encoding gene ( lsdB), starting 51 bp downstream of the lsdA gene, was cloned from strain SRT4. The lsdB gene (1605 bp) encodes a protein (calculated molecular mass 58.4 kDa) containing a putative 36-amino-acid s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0044-2
更新日期：2002-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006769
更新日期：1999-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9602-9
更新日期：2010-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006793
更新日期：1999-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9239-0
更新日期：2008-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010091
更新日期：2000-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4467-z
更新日期：2006-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1344-5
更新日期：2018-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00284-020-02221-x
更新日期：2020-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900272
更新日期：1998-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2004-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9142-8
更新日期：2008-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 新闻
doi：10.1007/s00284-003-4025-5
更新日期：2003-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0523-x
更新日期：2014-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900462
更新日期：1999-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02018-y
更新日期：2020-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3844-0
更新日期：2003-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910017
更新日期：2000-02-01 00:00:00
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更新日期：2001-11-01 00:00:00
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journal_title：Current microbiology
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更新日期：2011-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0333-2
更新日期：2006-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02028-w
更新日期：2020-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900268
更新日期：1997-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9268-8
更新日期：2009-01-01 00:00:00
abstract：:Polymorphisms in enzymatically amplified ribosomal DNA (rDNA) were examined in 18 strains of Saccharomyces. Restriction patterns generated from the region spanning the internal transcribed spacers (ITS) and the 5.8S rDNA produced two clusters corresponding to S. bayanus and S. cerevisiae. The type culture of S. carlsb...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01575857
更新日期：1992-11-01 00:00:00
abstract：:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0910-y
更新日期：2016-01-01 00:00:00
