Abstract:
:The Bacillus subtilis pur operon repressor (PurR) has a PRPP (5-phosphoribosyl 1-pyrophosphate) binding motif at residues 199-211. Two PurR PRPP binding region mutations (D203A and D204A) were constructed, and the effects on binding of repressor to the pur operon control site in vitro and on regulation of pur operon expression in vivo were investigated. PRPP significantly inhibited the binding of wild-type but not mutant PurR to pur operon control site DNA. In strains with the D203A and D204A mutations, pur operon expression in vivo was super-repressed by addition of adenine to the growth medium. These results support the role of PRPP in modulating the regulatory function of PurR in vivo. YabJ, the product of the distal gene in the bicistronic purR operon, is also required for PurR function in vivo.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Weng M,Zalkin Hdoi
10.1007/s002840010091keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
56-9issue
1eissn
0343-8651issn
1432-0991journal_volume
41pub_type
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