Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.

Abstract:

:The Bacillus subtilis pur operon repressor (PurR) has a PRPP (5-phosphoribosyl 1-pyrophosphate) binding motif at residues 199-211. Two PurR PRPP binding region mutations (D203A and D204A) were constructed, and the effects on binding of repressor to the pur operon control site in vitro and on regulation of pur operon expression in vivo were investigated. PRPP significantly inhibited the binding of wild-type but not mutant PurR to pur operon control site DNA. In strains with the D203A and D204A mutations, pur operon expression in vivo was super-repressed by addition of adenine to the growth medium. These results support the role of PRPP in modulating the regulatory function of PurR in vivo. YabJ, the product of the distal gene in the bicistronic purR operon, is also required for PurR function in vivo.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Weng M,Zalkin H

doi

10.1007/s002840010091

keywords:

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

56-9

issue

1

eissn

0343-8651

issn

1432-0991

journal_volume

41

pub_type

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