Effect of chlorhexidine on multi-species biofilms.

Abstract:

:With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve biofilms had higher proportions of streptococci but lower proportions of veillonellae than suc -ve biofilms. Exposure to CHG for 1 min reduced the viable count of suc -ve and suc +ve biofilms by 53% and 89% respectively. In both cases, reductions were mainly attributable to killing of streptococci and actinomyces. After 60 min of exposure, 4% of the bacteria in the suc -ve and 2% in the suc +ve biofilms remained viable. This study has shown that large numbers of bacteria in microcosm dental plaques can survive a 1-min exposure to 0.2% CHG and that even after a 60-min exposure, substantial numbers of bacteria remain viable.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Wilson M,Patel H,Noar JH

doi

10.1007/s002849900272

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

13-8

issue

1

eissn

0343-8651

issn

1432-0991

journal_volume

36

pub_type

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