Abstract：
:Actinomycetes are a wide group of Gram positive prokaryotes, the soil being their most characteristic habitat, where they play important ecological functions. Their immense biotechnological potential as producers of bioactive molecules of great commercial and industrial interest is exemplified by most antibiotics for clinical use being derived from this group of bacteria. In this work several methodologies and culture media were tested for the isolation of actinomycetes in soils from three different edafoclimatic areas of Tenerife (Canary Islands, Spain): an arid zone of the southeast coast of the island, a humid area in the laurel forest and the Canary high mountain. The results of this work evidenced that just an air drying of the sample during 7 days (pretreatment) produces high counts of actinomycetes versus non-actinomycetes in the three soils and in any of the culture media studied, making unnecessary post physical and chemical treatments. However, the only method that produced an exclusive isolation of actinomycetes was the use of 0.22-μm filters as a physical barrier, and hence being a tool of unquestionable utility to estimate actinomycetes populations in soil.The analysis by BOX rep-PCR determined that the richness and genetic diversity of the isolates of these localities are very high. The greatest similarities were, with one exception, found between strains from the same locations. Izaña was one that obtained the highest diversity. Regarding the biotecnology potential as antibiotic produces isolates from Güimar soils are the most promising as 7 out of 10 isolates showed some antibiotic activity.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Hernández-Bolaños E,Montesdeoca-Flores D,Abreu-Yanes E,Barrios ML,Abreu-Acosta Ndoi
10.1007/s00284-020-02030-2subject
Has Abstractpub_date
2020-09-01 00:00:00pages
2510-2522issue
9eissn
0343-8651issn
1432-0991pii
10.1007/s00284-020-02030-2journal_volume
77pub_type
杂志文章abstract：:Root colonization and diversity of arbuscular mycorrhizal fungi (AMF) were analyzed in plants growing in fly ash pond. Eight species could be separated morphologically, while phylogenetic analyses after PCR amplification of the ITS region followed by RFLP and sequencing revealed seven different AM fungal sequence type...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9974-5
更新日期：2011-09-01 00:00:00
abstract：:There is a growing interest in understanding the role of the gut microbiome on productive and meat quality-related traits in livestock species in order to develop new useful tools for improving pig production systems and industry. Faecal samples are analysed as a proxy of gut microbiota and here the selection of suita...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1429-1
更新日期：2018-06-01 00:00:00
abstract：:Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. dril...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0565-0
更新日期：2014-08-01 00:00:00
abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9333-3
更新日期：2009-05-01 00:00:00
abstract：:Haloarchaea are known for its adaptation in extreme saline environment. Halophilic archaea produces carotenoid pigments and proton pumps to protect them from extremes of salinity. Bacteriorhodopsin (bR) is a light-driven proton pump that resides in the membrane of haloarchaea Halobacterium salinarum. The photocycle of...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-017-1271-5
更新日期：2017-08-01 00:00:00
abstract：:The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900092
更新日期：1996-09-01 00:00:00
abstract：:The rates of urinary tract infection with multidrug-resistant (MDR) Escherichia coli have dramatically increased and the treatment of these infections with single and double antibiotic combinations became limited or ineffective. The present study aimed at finding effective antibiotic combinations against MDR uropathog...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01871-w
更新日期：2020-05-01 00:00:00
abstract：:The aim of the research was (i) to evaluate white and cream strains of A. bisporus yields when challenged or not with the pathogen L. fungicola, (ii) to identify the more aggressive pathogen isolates, and (iii) to develop a diagrammatic scale of spot symptoms on infected mushrooms. The experiment was carried out using...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02232-8
更新日期：2020-12-01 00:00:00
abstract：:The facultative anaerobe Pantoea sp. strain A-13, isolated from ornithogenic soil of Dewart Island (Frazier Islands), Antarctica, produced glycolipid biosurfactants when grown on n-paraffins or kerosene as the sole source of carbon and energy. Hemolysis of erythrocytes, growth inhibition of Bacillus subtilis, and thin...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0345-6
更新日期：2007-02-01 00:00:00
abstract：:Polymorphisms in enzymatically amplified ribosomal DNA (rDNA) were examined in 18 strains of Saccharomyces. Restriction patterns generated from the region spanning the internal transcribed spacers (ITS) and the 5.8S rDNA produced two clusters corresponding to S. bayanus and S. cerevisiae. The type culture of S. carlsb...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01575857
更新日期：1992-11-01 00:00:00
abstract：:Lactobacillus casei CRL705 produces a class IIb bacteriocin, lactocin 705, which relies on the complementary action of two components, Lac705alpha and Lac705beta. These peptides exert a bactericidal effect on the indicator strain Lactobacillus plantarum CRL691, with an optimal Lac705alpha/Lac705beta peptide ratio of 1...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3844-0
更新日期：2003-03-01 00:00:00
abstract：:The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9977-2
更新日期：2011-09-01 00:00:00
abstract：:Maize root colonization by two fluorescentPseudomonas strains M.3.1. and TR335, isolated respectively frommaize and tomato roots, were studied in hydroponic conditions. Each bacteriumwas inoculated separately, and three different colonization areas werestudied: nutrient solution, rhizoplane, and endorhizosphere. The t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900185
更新日期：1997-05-01 00:00:00
abstract：:Glutamine synthetase (GS) fromBeneckea alginolytica strain 90 was purified to homogeneity as indicated by sodium dodecyl sulface polyacrylamide gel electrophoresis. The purified enzyme was injected into rabbits, and the antiserum, as well as previously prepared antiserum against the GS fromEscherichia coli, was used i...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF02602446
更新日期：1980-07-01 00:00:00
abstract：:A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase ch...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900035
更新日期：1996-04-01 00:00:00
abstract：:Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9585-6
更新日期：2010-08-01 00:00:00
abstract：:The establishment of human flora-associated animal models allows the in vivo manipulation of host, microbial, and environmental parameters to influence the gut microbial community. However, it is difficult to simulate infant gut microbiota in germ-free animals because of the variation and dynamic state of infant micro...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0360-3
更新日期：2013-09-01 00:00:00
abstract：:Previously we isolated six actinomycetes strains, 9-4, 10-1, 10-2, 10-3, 10-6, and 21-4, that produce phospholipase D (PLD) with high transphosphatidylation activity. In this study, we identified these strains, and the PLD activities were compared with those of reference strains. 16S rDNA sequences and DNA-DNA hybridi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9551-3
更新日期：2010-05-01 00:00:00
abstract：:Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0512-9
更新日期：2007-04-01 00:00:00
abstract：:This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02212-y
更新日期：2020-12-01 00:00:00
abstract：:In this study, fatty-acid content and patterns were analyzed in order to distinguish Nostoc flagelliforme, an edible terrestrial cyanobacterium, from other Nostoc species and representatives typical of its close neighbors (genera Anabaena, Microcystis, and Synechococcus). According to the Kenyon-Murata classification ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4059-8
更新日期：2003-12-01 00:00:00
abstract：:Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsie...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9262-1
更新日期：2009-01-01 00:00:00
abstract：:Seventy-eight bacterial isolates from human, animal, and plant hosts, representing eight species of the family Enterobacteriaceae, were screened for F-like plasmid sequences. Of the examined human Escherichia coli strains, 28% harbored one or two of the three F-like, RepFI replication regions, while 35% of the examine...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0039-7
更新日期：2002-04-01 00:00:00
abstract：:Lactic acid bacteria such as Lactobacillus helveticus, L. delbrueckii subsp. delbrueckii, L. delbrueckii subsp. lactis, L. delbrueckii subsp. bulgaricus, L. acidophilus, and L. casei related taxa which are widely used as starter or probiotic cultures can be identified by amplified ribosomal DNA restriction analysis (A...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840110218
更新日期：2001-04-01 00:00:00
abstract：:Microbial populations in indoor environments, where we live and eat, are important for public health. Various bacterial species reside in the kitchen, and refrigerators, the major means of food storage within kitchens, can be a direct source of food borne illness. Therefore, the monitoring of microbiota in the refrige...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0401-y
更新日期：2013-11-01 00:00:00
abstract：:A Gram-positive, yellow pigmented actinobacterium, strain S2-48(T) was isolated from water sample collected from the river Brahmaputra, Assam, India and subjected to a polyphasic taxonomic study. Most of the physiological and biochemical properties, major fatty acids (C(15:0) Anteiso, iso C(16:0) and C(17:0) Anteiso),...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9822-z
更新日期：2011-03-01 00:00:00
abstract：:Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02068-2
更新日期：2020-10-01 00:00:00
abstract：:Recently, we showed that the southern green stink bug (SGSB), Nezara viridula (L.), can transmit Pantoea agglomerans (Ewing and Fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. Here, we hypothesized that our established model could be used to determine if the SGSB was a general, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9452-5
更新日期：2009-10-01 00:00:00
abstract：:Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900421
更新日期：1999-08-01 00:00:00
abstract：:To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900336
更新日期：1998-07-01 00:00:00
