Abstract：
:Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH range between 7 and 8. Comparative studies indicate that more than 60% of V. cholerae strains analyzed quantitatively produce more elastase in broth (two- to fourfold higher) than other elastase-positive Vibrio species such as Vibrio vulnificus. The V. cholerae elastase enzyme was not inhibited by trypsin, serine-protease, or thiol-protease inhibitors, but was inhibited by phosphoramidon. Ultrafiltration studies indicate the V. cholerae elastase enzyme has a molecular weight >30,000, and a 34K protein with possible elastase activity has been detected by SDS-PAGE for one non-O1 isolate (strain 2396). Cumulative results suggest that the V. cholerae elastase is probably a member of the N-type metalloprotease family and shares similar properties with other elastase enzymes described for pathogenic and nonpathogenic species in this genus.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Janda JM,Abbott SL,Khashe Sdoi
10.1007/s002849900421keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
73-8issue
2eissn
0343-8651issn
1432-0991pii
CM3010journal_volume
39pub_type
杂志文章abstract：:The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A to...
journal_title：Current microbiology
pub_type： 杂志文章
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journal_title：Current microbiology
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journal_title：Current microbiology
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pub_type： 信件
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更新日期：2009-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900029
更新日期：1996-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s002840010097
更新日期：2000-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900402
更新日期：1998-12-01 00:00:00
