Abstract：
:Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this study, the aim is to experimentally characterize a predicted spermidine synthase gene srm1, which was identified upon sequence similarity, in fission yeast Schizosaccharomyces pombe. In an attempt to understand the role of this gene in cell cycle and stress response, deletion mutant of srm1 was generated and analyzed in terms of cell cycle regulation and environmental stress response. The results showed that srm1Δ cells had elongated cell size and were sensitive to osmotic stress, while they showed no sensitivity to DNA-damaging agents. To the best of our knowledge, this is the first experimental characterization of srm1 gene and its role in cell cycle progression and stress response.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Gevrekci AÖdoi
10.1007/s00284-017-1241-ysubject
Has Abstractpub_date
2017-06-01 00:00:00pages
725-731issue
6eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1241-yjournal_volume
74pub_type
杂志文章abstract：:Two strains of the well-known insect pathogen and biopesticide, Bacillus thuringiensis (Bt), belonging to subspecies alesti (strain Bt5) and kurstaki (strain Bt213), were chosen for genetic characterization. The two strains belong to different serotypes and are currently classified into different subspecies, although ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0055-z
更新日期：2002-02-01 00:00:00
abstract：:The cluster of genes encoding components of the progenitor botulinum neurotoxin complex has been mapped and cloned in Clostridium botulinum type G strain ATCC 27322. Determination of the nucleotide sequence of the region has revealed open reading frames encoding nontoxic components of the complex, upstream of the gene...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900240
更新日期：1997-10-01 00:00:00
abstract：:Grasslands are suffering from long-term overgrazing because of the population inflation. Furthermore, nitrous oxide (N2O) is a major greenhouse gas that also depletes stratospheric ozone. However, the emission rate of grassland N2O and underlying mechanisms remained unclear under different grazing intensities. We cond...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01668-x
更新日期：2019-05-01 00:00:00
abstract：:Three yeast strains of Yamadazyma dushanensis f.a., sp. nov. were isolated from rotten wood samples collected in the Dushan Forest Park, Nanyang, Henan Province, China. Sequence analyses of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that this new species...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0847-1
更新日期：2015-08-01 00:00:00
abstract：:A moderately psychrophilic bacterium Corynebacterium paurometabolum MTCC 6841 (gram positive, short rod type) producing extracellular alkaline lipase was isolated from Lake Naukuchiatal, Uttaranchal, India. The bacterium was able to grow within a broad range of pH (5-10). Soyabean oil and olive oil served as the best ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0224-6
更新日期：2006-05-01 00:00:00
abstract：:Triterpenoid saponin derivatives oleanolic acid (OA) and ursolic acid (UA), but not betulinic acid (BA), were previously found to have strong antimicrobial activity against Streptococcus mutans. OA and UA inhibited the transcription of genes related to peptidoglycan biosynthesis, thereby preventing bacterial growth. H...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1344-5
更新日期：2018-01-01 00:00:00
abstract：:The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1350-7
更新日期：2018-01-01 00:00:00
abstract：:The influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from Lactobacillus fermentum CRL 251 was studied. Stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; sc...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900118
更新日期：1996-11-01 00:00:00
abstract：:GABA accumulation and glutamate decarboxylase (GAD) activity, the principal enzyme involved in GABA formation, was investigated in cyanobacterium Synechocystis sp. PCC 6803 wild-type (WT) and gad knockout mutant strains grown in medium containing different nitrogenous compounds. Nitrate was the best nitrogen source fo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0687-4
更新日期：2015-01-01 00:00:00
abstract：:Clostridium (Clostridioides) difficile ribotype 027 (RT027) was detected in Germany for the first time in 2007 during an outbreak in the region of Trier, Rhineland-Palatinate and is today the most prevalent ribotype (RT) in Europe. We aimed to determine the changes in RT distribution and corresponding antimicrobial re...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01654-3
更新日期：2019-04-01 00:00:00
abstract：:Iron-oxidizing Acidithiobacillus spp. are applied worldwide in biomining industry to extract metals from sulfide minerals. They derive energy for survival through Fe2+ oxidation and generate Fe3+ for the dissolution of sulfide minerals. However, molecular mechanisms of their iron oxidation still remain elusive. A nove...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1453-9
更新日期：2018-07-01 00:00:00
abstract：:(13)CNMR and (1)HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells. This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3944-x
更新日期：2003-07-01 00:00:00
abstract：:The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900258
更新日期：1997-11-01 00:00:00
abstract：:The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9351-1
更新日期：2009-05-01 00:00:00
abstract：:A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3978-0
更新日期：2003-08-01 00:00:00
abstract：:We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia, Cardinium, Rickettsia, Spiroplasma, Arsenophonus, and Hamiltonella. Our research, for the first time, revealed the presence of Cardinium in Mic...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01717-5
更新日期：2019-09-01 00:00:00
abstract：:A new cry gene (cry1Ca9) was cloned and sequenced from a Bacillus thuringiensis isolate native to Taiwan (G10-01A). The cry1C-type gene, designated cry1Ca9, consisted of an open reading frame of 3,567 bp, encoding a protein of 1,189 amino acid residues. The polypeptide has the deduced amino acid sequences predicting m...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3982-4
更新日期：2003-10-01 00:00:00
abstract：:Bats can be divided into frugivory, nectarivory, insectivory, and sanguivory based on their diets, and are therefore ideal wild animal models to study the relationship between diets and intestinal microflora. Early studies of bat gut bacteria showed that the diversity and structure of intestinal bacterial communities ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1530-0
更新日期：2018-10-01 00:00:00
abstract：:Growth on aniline by three purple non-sulfur bacteria (Rhodospirillum rubrum ATCC 11170, Rhodobacter sphaeroides DSM 158, and Rubrivivax benzoatiliticus JA2) as nitrogen, or carbon source could not be demonstrated. However in its presence, production of indole derivatives was observed with all the strains tested. At l...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9609-2
更新日期：2010-10-01 00:00:00
abstract：:The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crys...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0085-2
更新日期：2007-09-01 00:00:00
abstract：:Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02068-2
更新日期：2020-10-01 00:00:00
abstract：:Stenotrophomonas maltophilia is an emerging opportunistic pathogen linked not only to bacteremia, sepsis, and pneumonia but also to severe chronic enteritis. Persons with the impaired immune system are prone to be infected by S. maltophilia since its pathogenicity seems to be more associated with the host immune syste...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1559-0
更新日期：2018-12-01 00:00:00
abstract：:Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9347-x
更新日期：2009-04-01 00:00:00
abstract：:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0352-7
更新日期：2007-01-01 00:00:00
abstract：:Bacillus sp. B25 is an effective biocontrol agent against the maize pathogenic fungus Fusarium verticillioides (Fv). Previous in vitro assays have shown that B25 has protease, glucanase, and chitinase activities and siderophores production; however, specific mechanisms by which B25 controls Fv are still unknown. To de...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1372-1
更新日期：2018-03-01 00:00:00
abstract：:The limitation of phosphate concentrations in effluents is of international concern because of the risk of eutrophication.Acinetobacter spp. have often been isolated from activated sludge plants exhibiting enhanced phosphate removal. The ability to remove phosphate from mixed liquor byAcinetobacter isolates and refere...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01576016
更新日期：1993-09-01 00:00:00
abstract：:Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1208-z
更新日期：2017-04-01 00:00:00
abstract：:Streptococcus thermophilus (ST) chromosomal DNA fragments generated by partial Sau3A digestion were cloned into the unique BamHI site upstream from the promoterless chloramphenicol acetyltransferase (cat) gene of the Escherichia coli (EC)promoter-probe vector pKK520-3. Recombinant plasmids containing ST sequences with...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900171
更新日期：1997-04-01 00:00:00
abstract：:Bacterial FtsE gene codes for the ATP-binding protein, FtsE, which in complex with the transmembrane protein, FtsX, participates in diverse cellular processes. Therefore, regulated expression of FtsE and FtsX might be critical to the human pathogen, Mycobacterium tuberculosis, under stress conditions. Although ftsX ge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9897-1
更新日期：2011-05-01 00:00:00
abstract：:Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cy...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0383-9
更新日期：2013-10-01 00:00:00
