Abstract：
:This study assessed the performance of a new most probable number test (Pseudalert/Quanti-Tray) for the enumeration of Pseudomonas aeruginosa from swimming pool and spa pool waters by comparing it to the international and national membrane filtration-based culture methods for P. aeruginosa: ISO 16266:2006 and UK The Microbiology of Drinking Water-Part 8 (MoDW Part 8) which both use Pseudomonas CN agar. The comparison was based on the calculation of mean relative differences between the two methods conducted according to ISO 17994:2014. Using both routine pool water samples (149 from 8 laboratories) and artificially contaminated samples (309 from 7 laboratories), paired counts from each sample and enumeration method were analysed. For routine samples, there were insufficient data for a conclusive assessment, but the data do indicate at least equivalent performance of Pseudalert/Quanti-Tray to the reference methods. For the artificially contaminated samples, the data also did not result in a statistically conclusive assessment but did indicate potentially better performance of Pseudalert/Quanti-Tray. Combining the data from the routine samples and artificially contaminated samples resulted in an ISO 17994 outcome that the two methods were not statistically significantly different. Thus, the Pseudalert/Quanti-Tray method is an acceptable alternative to ISO 16266 and MoDW Part 8. The Pseudalert/Quanti-Tray method has the advantage in that it does not require confirmation testing, and of providing confirmed counts within 24-28 h incubation compared to 40-48 h or longer for the ISO 16266 and MoDW Part 8 methods.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Sartory DP,Brewer M,Beswick A,Steggles Ddoi
10.1007/s00284-015-0905-8subject
Has Abstractpub_date
2015-12-01 00:00:00pages
699-705issue
6eissn
0343-8651issn
1432-0991pii
10.1007/s00284-015-0905-8journal_volume
71pub_type
杂志文章abstract：:Maize root colonization by two fluorescentPseudomonas strains M.3.1. and TR335, isolated respectively frommaize and tomato roots, were studied in hydroponic conditions. Each bacteriumwas inoculated separately, and three different colonization areas werestudied: nutrient solution, rhizoplane, and endorhizosphere. The t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900185
更新日期：1997-05-01 00:00:00
abstract：:The addition of increasing physiological concentrations of ammonia or amino acids had distinct effects on the growth and proteolytic activity of Streptococcus bovis JB1, Prevotella albensis, and Butyrivibrio fibrisolvens DSM3071. The growth of S. bovis and B. fibrisolvens was enhanced by NH(3) and AA, and that of P. a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010074
更新日期：2000-06-01 00:00:00
abstract：:Forty-eight isolates resistant to at least two antibiotics were selected from 53 antibiotic-resistant enterococci from chicken and pig meat and faeces and analysed for specific resistance determinants. Of the 48 multidrug-resistant (MDR) strains, 31 were resistant to two antibiotics (29 to erythromycin and tetracyclin...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9880-x
更新日期：2011-05-01 00:00:00
abstract：:Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many species, including pigs, horses and humans. Biofilm formation is essential for pathogenesis, and the ability to resist antibiotic treatment results in difficult-to-treat and persistent infections. However, the ability o...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0574-z
更新日期：2014-09-01 00:00:00
abstract：:Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9602-9
更新日期：2010-10-01 00:00:00
abstract：:Magnetotactic bacteria (MTB) are capable of synthesizing nano-sized, intracellular membrane-bound magnetosomes. To learn more about the genetic factors involved in magnetosome formation, transposon mutagenesis was carried out by conjugation using a hyperactive mariner transposon to obtain nonmagnetic mutants of Magnet...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0131-6
更新日期：2012-07-01 00:00:00
abstract：:Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006756
更新日期：1998-03-01 00:00:00
abstract：:A gene encoding the rice (Oryza sativa L.) 90-kDa heat shock protein (OsHsp90) was introduced into Escherichia coli using the pGEX-6p-3 expression vector with a glutathione-S-transferase (GST) tag to analyze the possible function of this protein under heat stress for the first time. We compared the survivability of E....
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9288-4
更新日期：2009-02-01 00:00:00
abstract：:The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9977-2
更新日期：2011-09-01 00:00:00
abstract：:Two strains of a novel basidiomycetous yeast species were isolated from the gut of wood-boring larvae collected in the Baotianman Nature Reserve, the central China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) regions showed that these yeasts belong ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0203-7
更新日期：2012-11-01 00:00:00
abstract：:Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900462
更新日期：1999-11-01 00:00:00
abstract：:Corynebacterium glutamicum, a Gram-positive soil bacterium belonging to the mycolic acids-containing actinomycetes, is able to use the lignin degradation products ferulate, vanillate, and protocatechuate as sole carbon sources. The gene cluster responsible for vanillate catabolism was identified and characterized. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4531-8
更新日期：2005-07-01 00:00:00
abstract：:Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0355-9
更新日期：2006-05-01 00:00:00
abstract：:In laboratory cultures of ice nucleation-active (Ice+) Erwinia herbicola isolates, it has been difficult to achieve high-level expression of ice nuclei, especially nuclei active at temperatures warmer than -5 degrees C (i.e., type 1 ice nuclei). Here we demonstrate that starvation for phosphate and exposure to low tem...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900325
更新日期：1998-06-01 00:00:00
abstract：:This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02212-y
更新日期：2020-12-01 00:00:00
abstract：:We constructed recombinant phage particles displaying the Bacillus thuringiensis Cry1Ba4 active toxin using the pfUSE5 and pComb3X phagemid vectors. The recombinant phage particles were screened and evaluated for displayed biologically active Cry1Ba4 toxin against the target insect larvae. Concurrent expression of Cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0164-9
更新日期：2006-11-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake in the provided KEMB accession number. ...
journal_title：Current microbiology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00284-018-1592-z
更新日期：2019-01-01 00:00:00
abstract：:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294703
更新日期：1995-12-01 00:00:00
abstract：:The psychrotrophic bacterium Aeromonas hydrophila 7966 was subjected to cold shocks from 30 degrees C to 20 degrees C, 15 degrees C, 10 degrees C, or 5 degrees C, or were incubated at low temperature to determine its adaptative response. The cell protein patterns analyzed by two-dimensional electrophoresis revealed th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4277-8
更新日期：2004-08-01 00:00:00
abstract：:The adhesive property to the intestinal mucin of Bifidobacterium lactis LKM512, B. longum, B. breve, B. bifidum, B. adolescentis, B. infantis, Bacteroides vulgatus, Bacteroides distasonis, Eubacterium aerofaciens, Clostridium perfringens, Escherichia coli, and Lactobacillus acidophilus were examined. Adhesive rate of ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0087-4
更新日期：2002-03-01 00:00:00
abstract：:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0352-7
更新日期：2007-01-01 00:00:00
abstract：:Microbial populations in indoor environments, where we live and eat, are important for public health. Various bacterial species reside in the kitchen, and refrigerators, the major means of food storage within kitchens, can be a direct source of food borne illness. Therefore, the monitoring of microbiota in the refrige...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0401-y
更新日期：2013-11-01 00:00:00
abstract：:One hundred fifty-five isolates of Bipolaris sorokiniana of wheat were studied for their morphopathological characterization. These isolates were grouped in five categories--black, brown/dull black, gray cottony growth, dull white/greenish black, and white--on the basis of their growth pattern. The frequency of the bl...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0035-z
更新日期：2007-08-01 00:00:00
abstract：:The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01898-4
更新日期：2020-06-01 00:00:00
abstract：:Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01739-z
更新日期：2019-12-01 00:00:00
abstract：:Despite their ubiquity, relatively few bacteriophages have been characterized. Here, we set out to explore Caulobacter bacteriophages (caulophages) in the rhizosphere and characterized Kronos, the first caulophage isolated from the rhizosphere. Kronos is a member of the Siphoviridae family since it has a long flexible...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01656-1
更新日期：2019-05-01 00:00:00
abstract：:A novel bacterium, designated U33(T), was isolated from a soil sample collected in Mykhailyky, Poltavs'ka oblast, Ukraine. The bacterium was aerobic, Gram-positive, spore-forming, and consists of motile rods. The taxonomic position of strain U33(T) was studied by a polyphasic approach, and the results clearly showed t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0930-7
更新日期：2016-02-01 00:00:00
abstract：:A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9T belonge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1246-6
更新日期：2017-07-01 00:00:00
abstract：:Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice ve...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01570083
更新日期：1992-07-01 00:00:00
abstract：:The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacte...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010222
更新日期：2001-05-01 00:00:00
