Abstract：
:One hundred fifty-five isolates of Bipolaris sorokiniana of wheat were studied for their morphopathological characterization. These isolates were grouped in five categories--black, brown/dull black, gray cottony growth, dull white/greenish black, and white--on the basis of their growth pattern. The frequency of the black suppressed type was maximum (45.63%), whereas the white isolate displayed lowest frequency (6.96%) in the natural population. Twenty RAPD (random amplified polymorphic DNA) primers were used to observe the variability among the identified groups of B. sorokininana. From each group, eight random isolates were investigated. A total of 143 bands were amplified, out of which 107 (74.83%) were polymorphic and 36 (25.17%) were monomorphic. On an average, the total numbers of bands generated per primer were 7.15, of which 5.35 and 1.80 were polymorphic and monomorphic, respectively. Dendrograms based on molecular polymorphism unveiled a considerable amount of diversity among the isolates. Specific DNA bands were identified for selected isolates. The distinct markers appeared to be potential enough to be employed as genetic fingerprints for future strain identification and classification. The study indicated that the RAPD primers provide an easy, rapid, and simple technique for the preliminary assessment of genetic diversity among the fungal isolates.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Jaiswal SK,Sweta,Prasad LC,Sharma S,Kumar S,Prasad R,Pandey SP,Chand R,Joshi AKdoi
10.1007/s00284-007-0035-zsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
135-41issue
2eissn
0343-8651issn
1432-0991journal_volume
55pub_type
杂志文章abstract：:Thirteen clinical isolates of Pasteurella multocida from a variety of different animals and humans were examined for their ability to produce lipase. Lipase substrates used included Tween 20, Tween 40, Tween 80, and Tween 85. Lipase activity was detected in the filtrates of organisms grown to the exponential phase in ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910061
更新日期：2000-05-01 00:00:00
abstract：:A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0495-2
更新日期：2014-04-01 00:00:00
abstract：:A Gram-negative, yellow pigmented bacterium designated strain CA5(T) that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9745-8
更新日期：2011-02-01 00:00:00
abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0977-5
更新日期：2016-04-01 00:00:00
abstract：:Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9158-0
更新日期：2008-08-01 00:00:00
abstract：:A Gram-positive bacterium, designated strain XY18(T), was isolated from a cured vanilla bean in Hainan province, China. Cells were rod-shaped, endospore producing, and peritrichous flagella. Strain XY18(T) grew at salinities of 0-8 % (w/v) NaCl (optimally 1-4 %), pH 4.0-8.0 (optimally 5.0-7.0 %) and temperature range ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0707-4
更新日期：2015-02-01 00:00:00
abstract：:Enterohemorrhagic Escherichia coli (E. coli) O157:H7, a gram-negative bacteria identified as a foodborne pathogen causing severe disease is of great concern worldwide. The pathogenicity of E. coli O157:H7 is due to the presence of some virulence factors and its ability to form biofilm which resist antimicrobial compou...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-017-1314-y
更新日期：2017-12-01 00:00:00
abstract：:The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9643-0
更新日期：2010-12-01 00:00:00
abstract：:A Pseudomonas sp. that may be useful in bioremediation projects was isolated from soil. The strain is of potential value because it reduces selenite to elemental red selenium and is unusual in that it was resistant to high concentrations of both selenate and selenite. Exposure of the strain to 50, 100, and 150 mM sele...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9358-2
更新日期：2009-05-01 00:00:00
abstract：:The aim of this study is to investigate the combination of cefepime and sulbactam. Sulbactam, when administered , will effectively inhibit all Extended-spectrum beta lactamases (ESBLs) of the microorganism, while cefepime will inhibit the growth of the resistant microorganisms since it will not be hydrolyzed by OXA-48...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02094-0
更新日期：2020-09-01 00:00:00
abstract：:Production of carbon dioxide, as one of the ultimate products of fungal metabolism, can be used to quantify and measure their metabolic rate under different conditions, thus aiding in finding the optimal substrate and environment for cultivation of wood-destroying fungi. This study is focused on species Pleurotus ostr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02033-z
更新日期：2020-09-01 00:00:00
abstract：:Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0355-9
更新日期：2006-05-01 00:00:00
abstract：:A moderately psychrophilic bacterium Corynebacterium paurometabolum MTCC 6841 (gram positive, short rod type) producing extracellular alkaline lipase was isolated from Lake Naukuchiatal, Uttaranchal, India. The bacterium was able to grow within a broad range of pH (5-10). Soyabean oil and olive oil served as the best ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0224-6
更新日期：2006-05-01 00:00:00
abstract：:The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4358-8
更新日期：2004-10-01 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen, which can cause severe urinary tract infections (UTIs). Because of the high intrinsic antibiotic resistance of P. aeruginosa and its ability to develop new resistances during antibiotic treatment, these infections are difficult to eradicate. The antibiotic sus...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0026-y
更新日期：2012-01-01 00:00:00
abstract：:Zoospores of Chlamydomonas reinhardtii exhibit chemotaxis towards maltose, sucrose, xylose, mannitol, and ammonium. Ten independent mutants defective in chemotaxis towards sugars have been isolated. These mutants form five phenotypic classes. Genetic analysis of two mutant strains defective in chemotaxis to maltose (C...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900063
更新日期：1996-06-01 00:00:00
abstract：:Strain YA was newly isolated from an enrichment culture of river sediment and was identified as Janibacter sp. It was able to utilize dibenzofuran as the sole source of carbon and energy. Strain YA degraded > 90% of 1-chloro-dibenzo-p-dioxin (1-CDD) and > 80% of 2-chloro-dibenzo-p-dioxin in 18 hours with each initial ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0099-6
更新日期：2005-11-01 00:00:00
abstract：:The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9831-y
更新日期：2011-04-01 00:00:00
abstract：:A new cry gene (cry1Ca9) was cloned and sequenced from a Bacillus thuringiensis isolate native to Taiwan (G10-01A). The cry1C-type gene, designated cry1Ca9, consisted of an open reading frame of 3,567 bp, encoding a protein of 1,189 amino acid residues. The polypeptide has the deduced amino acid sequences predicting m...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3982-4
更新日期：2003-10-01 00:00:00
abstract：:The addition of increasing physiological concentrations of ammonia or amino acids had distinct effects on the growth and proteolytic activity of Streptococcus bovis JB1, Prevotella albensis, and Butyrivibrio fibrisolvens DSM3071. The growth of S. bovis and B. fibrisolvens was enhanced by NH(3) and AA, and that of P. a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010074
更新日期：2000-06-01 00:00:00
abstract：:The mutation of Agrobacterium radiobacter cells exposed to chrysotile asbestos was examined by the random amplified polymorphic DNA (RAPD) method. Approximately 1.4 kbp of DNA in A. radiobacter, which was not amplified strongly in the cells that were not exposed to asbestos, was amplified in the cells that were expose...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00021058
更新日期：2001-06-01 00:00:00
abstract：:Nine phage isolates infectious for Vibrio vulnificus and falling into four morphological groups were isolated from estuarine waters collected in Louisiana. Of the 60 V. vulnificus strains tested, 87% were susceptible to one or more of the isolates. With the exception of V. fluvialis, Vibrio species other than vulnific...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00369859
更新日期：1995-06-01 00:00:00
abstract：:In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0192-x
更新日期：2006-06-01 00:00:00
abstract：:The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010199
更新日期：2001-03-01 00:00:00
abstract：:This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9098-8
更新日期：2008-09-01 00:00:00
abstract：:Findings show 21 fungal isolates belonging to eight genera recovered from Egyptian soils that have the potential to attack L-methionine under submerged conditions. Aspergillus flavipes had the most methioninolytic activity, giving the highest yield of L-methioninase (10.78 U/mg protein), rate of methionine uptake (93....
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9311-9
更新日期：2009-03-01 00:00:00
abstract：:Lactobacillus casei CRL705 produces a class IIb bacteriocin, lactocin 705, which relies on the complementary action of two components, Lac705alpha and Lac705beta. These peptides exert a bactericidal effect on the indicator strain Lactobacillus plantarum CRL691, with an optimal Lac705alpha/Lac705beta peptide ratio of 1...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3844-0
更新日期：2003-03-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9608-3
更新日期：2010-10-01 00:00:00
abstract：:The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0021-9
更新日期：2002-05-01 00:00:00
abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
