Abstract:
:The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had only 2.8%. Meantime, the aqueous/isooctane (1:1) biphasic system was used for the further improvement of selectivity. By these methods, nearly 100% selectivity and conversion rate could be obtained within 1 h at the optimum initial benzyl alcohol concentration of 5.0 g/l.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Wu J,Li MH,Lin JP,Wei DZdoi
10.1007/s00284-010-9831-ysubject
Has Abstractpub_date
2011-04-01 00:00:00pages
1123-7issue
4eissn
0343-8651issn
1432-0991journal_volume
62pub_type
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