Highly selective oxidation of benzyl alcohol using engineered Gluconobacter oxydans in biphasic system.

Abstract:

:The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had only 2.8%. Meantime, the aqueous/isooctane (1:1) biphasic system was used for the further improvement of selectivity. By these methods, nearly 100% selectivity and conversion rate could be obtained within 1 h at the optimum initial benzyl alcohol concentration of 5.0 g/l.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Wu J,Li MH,Lin JP,Wei DZ

doi

10.1007/s00284-010-9831-y

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

1123-7

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

62

pub_type

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