Highly selective oxidation of benzyl alcohol using engineered Gluconobacter oxydans in biphasic system.


:The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had only 2.8%. Meantime, the aqueous/isooctane (1:1) biphasic system was used for the further improvement of selectivity. By these methods, nearly 100% selectivity and conversion rate could be obtained within 1 h at the optimum initial benzyl alcohol concentration of 5.0 g/l.


Curr Microbiol


Current microbiology


Wu J,Li MH,Lin JP,Wei DZ




Has Abstract


2011-04-01 00:00:00












  • Purification and identification of a novel leucine aminopeptidase from Bacillus thuringiensis israelensis.

    abstract::A novel leucine aminopeptidase was purified from a Bacillus thuringiensis israelensis (Bti) culture. The purification stages included heating the concentrated supernatant to 65 degrees C for 90 min, anion-exchange chromatography by DEAE cellulose, and hydrophobic chromatography by phenyl Sepharose. The specific activi...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Cahan R,Hetzroni E,Nisnevitch M,Nitzan Y

    更新日期:2007-11-01 00:00:00

  • Production of polysaccharidases in different carbon sources by Leucoagaricus gongylophorus Möller (Singer), the symbiotic fungus of the leaf-cutting ant Atta sexdens Linnaeus.

    abstract::Leucoagaricus gongylophorus, the fungus cultured by the leaf-cutting ant Atta sexdens, produces polysaccharidases that degrade leaf components by generating nutrients believed to be essential for ant nutrition. We evaluated pectinase, amylase, xylanase, and cellulase production by L. gongylophorus in laboratory cultur...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Silva A,Bacci M Jr,Pagnocca FC,Bueno OC,Hebling MJ

    更新日期:2006-07-01 00:00:00

  • Clone Q379 immunoscreened from a Chinese HCV cDNA lambda gt11 library.

    abstract::A Chinese HCV cDNA clone, designated as Q379, 379 nucleotides in length, was selected by recombinant immunoscreening from a random-primed Chinese HCV lambda gt11 library. DNA sequencing demonstrated that Q379 situated from positions 7314 to 7695 at NS5 region corresponded with the American prototype HCV nucleotide seq...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Qi Z,Arima T

    更新日期:1994-03-01 00:00:00

  • Isolation and characterization of new cyclohexylacetic acid-degrading bacteria.

    abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Iwaki H,Nakai E,Nakamura S,Hasegawa Y

    更新日期:2008-08-01 00:00:00

  • Correction to: Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers.

    abstract::The authors would like to correct the errors in the publication of the original article. ...

    journal_title:Current microbiology

    pub_type: 杂志文章,已发布勘误


    authors: Neveling DP,Ahire JJ,Laubscher W,Rautenbach M,Dicks LMT

    更新日期:2020-08-01 00:00:00

  • Cloning of lipoxygenase genes from a cyanobacterium, Nostoc punctiforme, and its expression in Eschelichia coli.

    abstract::Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Koeduka T,Kajiwara T,Matsui K

    更新日期:2007-04-01 00:00:00

  • Analysis of the Endophytic Bacteria Community Structure and Function of Panax notoginseng Based on High-Throughput Sequencing.

    abstract::Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Zhang C,Ma X,Zhu R,Liu Z,Gu M,Zhang J,Li Y,Xu Y,Zhu D

    更新日期:2020-10-01 00:00:00

  • Complete Genome Sequences of Leclercia sp. W6 and W17 Isolated from a Gastric Cancer Patient.

    abstract::Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Xu YY,Huang CJ,Xu L,Jiang XW,Xu XW,Xu XW

    更新日期:2020-10-01 00:00:00

  • Extended serotyping scheme for Vibrio anguillarum with the definition and characterization of seven provisional O-serogroups.

    abstract::The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Pedersen K,Grisez L,van Houdt R,Tiainen T,Ollevier F,Larsen JL

    更新日期:1999-03-01 00:00:00

  • Spiking a Silty-Sand Reference Soil with Bacterial DNA: Limits and Pitfalls in the Discrimination of Live and Dead Cells When Applying Ethidium Monoazide (EMA) Treatment.

    abstract::In the present study, EMA (ethidium monoazide) treatment was applied to a silty-sand reference soil prior to DNA extraction to enable a differentiation between dead and living cells. For this purpose, a reference soil was spiked with Listeria monocytogenes cells or cell equivalents, respectively. With the purpose of e...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Wagner AO,Praeg N,Illmer P

    更新日期:2019-12-01 00:00:00

  • Stratified communities of active archaea in shallow sediments of the Pearl River Estuary, Southern China.

    abstract::Marine subsurface sediments represent a novel archaeal biosphere with unknown physiology. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of active archaea in a sediment core were ch...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Chen J,Wang F,Jiang L,Yin X,Xiao X

    更新日期:2013-07-01 00:00:00

  • Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells.

    abstract::An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemi...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Choudhuri I,Khanra K,Pariya P,Maity GN,Mondal S,Pati BR,Bhattacharyya N

    更新日期:2020-10-01 00:00:00

  • Disruption of a global regulatory gene to enhance central carbon flux into phenylalanine biosynthesis in Escherichia coli.

    abstract::Genetic engineering of microbes for commercial metabolite production traditionally has sought to alter the levels and/or intrinsic activities of key enzymes in relevant biosynthetic pathway(s). Microorganisms exploit similar strategies for flux control, but also coordinate flux through sets of related pathways by usin...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Tatarko M,Romeo T

    更新日期:2001-07-01 00:00:00

  • Cadmium-induced siderophore production by a high Cd-resistant bacterial strain relieved Cd toxicity in plants through root colonization.

    abstract::This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Sinha S,Mukherjee SK

    更新日期:2008-01-01 00:00:00

  • Single-Nucleotide Polymorphisms in the Whole-Genome Sequence Data of Shiga Toxin-Producing Escherichia coli O157:H7/H- Strains by Cultivation.

    abstract::Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Yokoyama E,Hirai S,Ishige T,Murakami S

    更新日期:2017-04-01 00:00:00

  • Ruminal Microbial Populations and Fermentation Characteristics in Beef Cattle Grazing Tropical Forage in Dry Season and Supplemented with Different Protein Levels.

    abstract::We tested the hypothesis that supplementation with protein improves fermentation parameters without damaging the rumen microbial populations of beef cattle grazing Urochloa brizantha cv. Marandu during the dry season. Four rumen-cannulated Nellore bulls (571 ± 31 kg of body weight) were used in a 4 × 4 Latin square de...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: da Silva-Marques RP,Zervoudakis JT,Nakazato L,Hatamoto-Zervoudakis LK,da Silva Cabral L,do Nascimento Matos NB,da Silva MIL,Feliciano AL

    更新日期:2019-03-01 00:00:00

  • Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea.

    abstract::Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage tite...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Li Y,Wang M,Liu Q,Song X,Wang D,Ma Y,Shao H,Jiang Y

    更新日期:2016-05-01 00:00:00

  • Characterization of Comamonas thiooxidans sp. nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti.

    abstract::Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Narayan KD,Pandey SK,Das SK

    更新日期:2010-10-01 00:00:00

  • Microbial Communities of the Gut and Nest of the Humus- and Litter-Feeding Termite Procornitermes araujoi (Syntermitinae).

    abstract::The evolution of the symbiotic association with microbes allowed termites to decompose ingested lignocellulose from plant-derived substrates, including herbivore dung and soil humus. Representatives of the Syntermitinae (Termitidae) range in their feeding habits from wood and litter-feeding to humus-feeding species. H...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Moreira EA,Alvarez TM,Persinoti GF,Paixão DAA,Menezes LR,Cairo JPF,Squina FM,Costa-Leonardo AM,Carrijo T,Arab A

    更新日期:2018-12-01 00:00:00

  • Phase variations in Bifidobacterium animalis.

    abstract::Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice ve...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Biavati B,Crociani F,Mattarelli P,Scardovi V

    更新日期:1992-07-01 00:00:00

  • Fungal communities in decaying sapwood and heartwood of a conifer Keteleeria evelyniana.

    abstract::Fungal communities in decaying sapwood and heartwood of K. evelyniana were demonstrated through construction of four 18 S rRNA gene libraries. The 210 sequenced clones were clustered into 11 subgroups, belonging to Basidiomycota (71.9%) and to Ascomycota (22.4%) and unclassified (1 subgroup; 5.7%). The heartwood displ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Zhang HB,Yang MX,Tu R,Gao L,Zhao ZW

    更新日期:2008-04-01 00:00:00

  • Cys92, Cys101, Cys197, and Cys203 are crucial residues for coordinating the iron-sulfur cluster of RhdA from Acidithiobacillus ferrooxidans.

    abstract::By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Dai Y,Liu J,Zheng C,Wu A,Zeng J,Qiu G

    更新日期:2009-11-01 00:00:00

  • Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and synergism of their encoded polypeptides.

    abstract::A thermophilic bacterial strain GXN151 which could degrade Avicel efficiently was isolated and identified as Bacillus licheniformis. A genomic library of GXN151 was constructed and two novel endoglucanase genes designated cel9A and cel12A were isolated by screening the library on carboxylmethyl cellulase indicator pla...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Liu Y,Zhang J,Liu Q,Zhang C,Ma Q

    更新日期:2004-10-01 00:00:00

  • Field trial of biocides for control of Legionella in cooling towers.

    abstract::Legionella and amebae populations in 16 cooling towers were challenged with three commercially available biocide formulations. The active agents were: a chlorinated phenolic thioether (CPTE), bromo-nitro-propane-diol (BNPD), and bromo-chloro-dimethylhydantoin (BCD, in briquette form). The towers were dosed with these ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Bentham RH,Broadbent CR

    更新日期:1995-03-01 00:00:00

  • Adherence inhibition of Cronobacter sakazakii to intestinal epithelial cells by lactoferrin.

    abstract::Cronobacter sakazakii is now recognized as an opportunistic pathogen and has been implicated in rare but severe cases of necrotizing enterocolitis, meningitis, and sepsis in neonates. The first step in bacterial pathogenesis requires that the organism adheres to host cells surfaces; therefore, agents that inhibit adhe...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Quintero-Villegas MI,Wittke A,Hutkins R

    更新日期:2014-10-01 00:00:00

  • Regulation of sodium influx in the NaCl-resistant (NaCl(r)) mutant strain of the cyanobacterium Anabaena variabilis.

    abstract::A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Chauhan VS,Singh B,Singh S,Bisen PS

    更新日期:2001-02-01 00:00:00

  • The tracing of mycobacteria in drinking water supply systems by culture, conventional, and real time PCRs.

    abstract::Mycobacteria are widely present in diverse aquatic habitats, where they can survive for months or years while some species can even proliferate. The resistance of different mycobacterial species to disinfection methods like chlorination or ozonation could result in their presence in the final tap water of consumers. I...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Klanicova B,Seda J,Slana I,Slany M,Pavlik I

    更新日期:2013-12-01 00:00:00

  • The ability of 2-deoxyglucose to promote the lysis of Streptococcus bovis JB1 via a mechanism involving cell wall stability.

    abstract::The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Russell JB,Wells JE

    更新日期:1997-11-01 00:00:00

  • Impact of an herbicide combination of bromoxynil and prosulfuron on soil microorganisms.

    abstract::Soil microcosm experiments were used to investigate the effects on growth and activity of soil microorganisms of an herbicide combination (60% bromoxynil + 3% prosulfuron) frequently used to provide a broad spectrum control of weed species. Culturable aerobic bacteria, fungi, and actinomycetes, the fundamental groups ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Pampulha ME,Oliveira A

    更新日期:2006-09-01 00:00:00

  • Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomic Status and Sulfur Metabolism Potential.

    abstract::Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Akhtar N,Ghauri MA,Akhtar K,Parveen S,Farooq M,Ali A,Schierack P

    更新日期:2019-10-01 00:00:00