DNA extraction from activated sludges.

abstract：:In this study, the effect of rhamnolipid biosurfactant produced by Pseudomonas fluorescens on bacterial strains, laboratory strains, and isolates from industrial wastewater was investigated. It was shown that biosurfactant, depending on the concentration, has a neutral or detrimental effect on the growth and protein r...

journal_title：Current microbiology

authors： Vasileva-Tonkova E,Sotirova A,Galabova D

更新日期：2011-02-01 00:00:00

abstract：:Temperature and salinity fluctuations are two of the most important factors affecting the growth of polar bacteria. In an attempt to better understand the function of heat-shock proteins (HSPs) in the adaptive mechanisms of the Antarctic psychrotrophic bacterium Psychrobacter sp. G to such conditions, genes Hsp845, Hs...

journal_title：Current microbiology

authors： Che S,Song W,Lin X

更新日期：2013-11-01 00:00:00

abstract：:The neurotoxin gene of non-proteolytic Clostridium botulinum type B (strain Eklund 17B) was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated with primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 3' end of the gene was obtained by using primer...

journal_title：Current microbiology

authors： Hutson RA,Collins MD,East AK,Thompson DE

更新日期：1994-02-01 00:00:00

abstract：:Avian pathogenic Escherichia coli (APEC) is one of the pathogens that most concerns the poultry industry worldwide due to the economic losses it can cause. APEC persistence and survival, both in the environment and in the host, may be a consequence of biofilm-producing capabilities. The aim of this study was to evalua...

journal_title：Current microbiology

authors： Rodrigues SV,Laviniki V,Borges KA,Furian TQ,Moraes HLS,Nascimento VP,Salle CTP

更新日期：2019-02-01 00:00:00

abstract：:A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with ...

journal_title：Current microbiology

authors： Sørensen SJ,Sørensen AH,Hansen LH,Oregaard G,Veal D

更新日期：2003-08-01 00:00:00

abstract：:To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...

journal_title：Current microbiology

authors： Brambila-Tapia AJ,Poot-Hernández AC,Garcia-Guevara JF,Rodríguez-Vázquez K

更新日期：2016-06-01 00:00:00

abstract：:Magnetotactic bacteria (MTB) are capable of synthesizing nano-sized, intracellular membrane-bound magnetosomes. To learn more about the genetic factors involved in magnetosome formation, transposon mutagenesis was carried out by conjugation using a hyperactive mariner transposon to obtain nonmagnetic mutants of Magnet...

journal_title：Current microbiology

authors： Bo T,Wang K,Ge X,Chen G,Liu W

更新日期：2012-07-01 00:00:00

abstract：:Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding s...

journal_title：Current microbiology

authors： Kiser KB,Schmidt MG

更新日期：1999-02-01 00:00:00

abstract：:Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective ...

journal_title：Current microbiology

authors： Champney WS,Tober CL,Burdine R

更新日期：1998-12-01 00:00:00

abstract：:The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...

journal_title：Current microbiology

authors： Prasad S,Pratibha MS,Manasa P,Buddhi S,Begum Z,Shivaji S

更新日期：2013-01-01 00:00:00

abstract：:All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...

journal_title：Current microbiology

authors： Xu L,Han G,Fan X,Lv J,Zhang X,Peng Q,Zhang J,Xu J,Song F

更新日期：2020-11-01 00:00:00

abstract：:PHA is one of the leading commercially important bio-polyesteric compounds piled up as an intracellular lipid-based energy storage compound by numerous microorganisms. An indigenous Gram-positive bacterium isolated from fire ant (Solenopsis invicta) has known to potentially accumulate PHA. Various nutritional elements...

journal_title：Current microbiology

authors： Suguna PP,Saranya VV,Abirami PP,Shenbagarathai RR

更新日期：2020-12-01 00:00:00

abstract：:Zoospores of Chlamydomonas reinhardtii exhibit chemotaxis towards maltose, sucrose, xylose, mannitol, and ammonium. Ten independent mutants defective in chemotaxis towards sugars have been isolated. These mutants form five phenotypic classes. Genetic analysis of two mutant strains defective in chemotaxis to maltose (C...

journal_title：Current microbiology

authors： Ermilova EV,Chekunova EM,Zalutskaya ZM,Krupnov KR,Gromov BV

更新日期：1996-06-01 00:00:00

abstract：:PCR has been widely used to identify cry-type genes, to determine their distribution, to detect new such genes and to predict insecticidal activities. We describe here a molecular approach to analyze the genetic diversity of B. thuringiensis cry-like genes based on denaturing gradient gel electrophoresis (DGGE). This ...

journal_title：Current microbiology

authors： Vidal-Domínguez ME,Perez-Cenci M,Salerno GL,Berón CM

更新日期：2011-03-01 00:00:00

abstract：:Yarrowia lipolytica is a dimorphic fungus that secretes either an acidic or an alkaline protease depending on the environmental pH. Previous results have indicated that secretion of the alkaline protease is under control of the pH signaling Pal/Rim pathway originally described in Aspergillus nidulans. Several Y. lipol...

journal_title：Current microbiology

authors： González-López CI,Ortiz-Castellanos L,Ruiz-Herrera J

更新日期：2006-07-01 00:00:00

abstract：:The diversities of soil bacterial communities in the Phragmites australis, Phalaris arundinacea, and non-plantation constructed wetlands were compared and analyzed by through high-throughput Illumina sequencing. At the phylum level, the ten dominant bacterial phyla in the three types of constructed wetlands were the s...

journal_title：Current microbiology

authors： Fu W,Wang Y,Wei W,Li P

更新日期：2019-03-01 00:00:00

abstract：:Debaryomyces nepalensis, a halotolerant food-spoiling yeast could grow in complex (YEPD) medium at different pHs ranging between 3.0 and 11.0 in the absence of salt and at pH 3.0-9.0 in the presence of different concentrations of NaCl and KCl. The specific growth rate of D. nepalensis was not affected by the initial p...

journal_title：Current microbiology

authors： Kumar S,Lal P,Gummadi SN

更新日期：2008-12-01 00:00:00

abstract：:The immunosuppressant leflunomide (LFM) inhibits the growth of cytokine-stimulated proliferation of lymphoid cells in vitro and also inhibits the growth of eukaryotic microorganism, Saccharomyces cerevisiae. As a first step to elucidate the molecular mechanism of LFM action in human, yeast gene which suppresses the an...

journal_title：Current microbiology

authors： Fujimura H

更新日期：2001-08-01 00:00:00

abstract：:The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...

journal_title：Current microbiology

authors： Ferrario C,Borgo F,de Las Rivas B,Muñoz R,Ricci G,Fortina MG

更新日期：2014-03-01 00:00:00

abstract：:The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacte...

journal_title：Current microbiology

authors： Bernardelli CE,Luna MF,Galar ML,Boiardi JL

更新日期：2001-05-01 00:00:00

abstract：:Cyanide has been proved to be degraded by Klebsiella oxytoca. In order to examine the physiological responses of cyanide degradation by this bacterium, two-dimensional (2-DE) electrophoresis approach and MALDI-TOF-MS allow us to identify 106 proteins spots that were significantly altered in the presence of 1 mM cyanid...

journal_title：Current microbiology

authors： Tang P,Hseu YC,Chou HH,Huang KY,Chen SC

更新日期：2010-03-01 00:00:00

abstract：:Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with ...

journal_title：Current microbiology

authors： Akhtar N,Ghauri MA,Akhtar K,Parveen S,Farooq M,Ali A,Schierack P

更新日期：2019-10-01 00:00:00

abstract：:Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observe...

journal_title：Current microbiology

authors： Novotná J,Li XM,Novotná J,Vohradský J,Weiser J

更新日期：1995-08-01 00:00:00

abstract：:Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...

journal_title：Current microbiology

authors： Yu X,Liu T,Sun Z,Guan P,Zhu J,Wang S,Li S,Deng Q,Wang L,Zheng A,Li P

更新日期：2012-04-01 00:00:00

abstract：:The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was...

journal_title：Current microbiology

authors： Krohn BM,Lindsay JA

更新日期：1993-04-01 00:00:00

abstract：:Candida galli strain PGO6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. The products were confirmed by thin-layer chromatography (TLC), changes in the UV absorption pattern and high-performance liquid chr...

journal_title：Current microbiology

authors： Ashengroph M,Nahvi I,Zarkesh-Esfahani H,Momenbeik F

更新日期：2011-03-01 00:00:00

abstract：:Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted levansucrase (LsdA). A levanase-encoding gene ( lsdB), starting 51 bp downstream of the lsdA gene, was cloned from strain SRT4. The lsdB gene (1605 bp) encodes a protein (calculated molecular mass 58.4 kDa) containing a putative 36-amino-acid s...

journal_title：Current microbiology

authors： Menéndez C,Hernández L,Selman G,Mendoza MF,Hevia P,Sotolongo M,Arrieta JG

更新日期：2002-07-01 00:00:00

abstract：:Bradysia difformis is one of the most damaging pests in mushroom production in China. In this study, eight Bacillus thuringiensis strains were analyzed for insecticidal activity in B. difformis. The strain JW-1 showed the highest insecticidal activity against B. difformis larvae, but did not inhibit the mycelial growt...

journal_title：Current microbiology

authors： Wang FF,Qu SX,Lin JS,Li HP,Hou LJ,Jiang N,Luo X,Ma L

更新日期：2020-10-01 00:00:00

abstract：:A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71T, was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high sim...

journal_title：Current microbiology

authors： Lim YK,Park SN,Shin JH,Ji S,Jo E,Chang YH,Shin Y,Paek J,Kim H,Kook JK

更新日期：2019-12-01 00:00:00

abstract：:In the present work, we provide biological evidences supporting the participation of NCW2 gene in the mechanism responsible for cell tolerance to polyhexamethylene biguanide (PHMB), an antifungal agent. The growth rate of yeast cells exposed to this agent was significantly reduced in ∆ncw2 strain and the mRNA levels o...

journal_title：Current microbiology

authors： Elsztein C,de Lima RCP,de Barros Pita W,de Morais MA Jr

更新日期：2016-09-01 00:00:00