Genetic relatedness of clinical and environmental Vibrio cholerae isolates based on triple housekeeping gene analysis.

abstract：:Rep22 is the replication region of the lactococcal theta replicating pUCL22 plasmid. The copy number of Rep22-based plasmids in Lactococcus was determined by using a chromosomal DNA fragment from Lactococcus lactis subsp. lactis MMS368 as reference. Segregational behavior appeared to be linked to copy number and there...

journal_title：Current microbiology

authors： Frère J,Herreman C,Boutibonnes P,Novel M,Novel G

更新日期：1995-01-01 00:00:00

abstract：:We develop a method to capture marine bacterial strains at high efficiency to replace the conventional two-step collecting method. Lab-made, Fe3O4 magnetic beads were used to firstly verify the feasibility of capture in artificial seawater, using Bacillus velezensis. Almost 100% of the bacteria could be captured and s...

journal_title：Current microbiology

authors： Zheng Y,Jian X,Xing XH,Zhang C

更新日期：2020-07-01 00:00:00

abstract：:Predation by Bdellovibrio-like organisms (BLOs) results in bacterial community succession in aquatic ecosystems. The effects of nutrient loading on the distribution and phylogeny of BLOs remain largely unknown. To this end, we present our findings on BLO diversity from four north-Indian lakes that are variable in thei...

journal_title：Current microbiology

authors： Chauhan A,Fortenberry GZ,Lewis DE,Williams HN

更新日期：2009-07-01 00:00:00

abstract：:Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...

journal_title：Current microbiology

authors： Kapur M,Bhatia R,Pandey G,Pandey J,Paul D,Jain RK

更新日期：2010-08-01 00:00:00

abstract：:Using a previously created Mycobacterium avium genomic library with GFP-promoter trap in Mycobacterium smegmatis, we screened for genes that are upregulated upon infection of A. castellanii. Clones exhibiting a 2.5-fold or greater increase in GFP expression, out of a total of 10,000 clones, were selected for further e...

journal_title：Current microbiology

authors： Tenant R,Bermudez LE

更新日期：2006-02-01 00:00:00

abstract：:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...

journal_title：Current microbiology

authors： Porteous LA,Armstrong JL,Seidler RJ,Watrud LS

更新日期：1994-11-01 00:00:00

abstract：:Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacI(q) gene and a re-engineered coliphage T5...

journal_title：Current microbiology

authors： Alqublan H,Seleem MN,Boyle SM,Sriranganathan N

更新日期：2010-04-01 00:00:00

abstract：:Lake Elmenteita is one of the alkaline saline lakes within the Kenyan Rift valley. The lake is situated on the floor of the Kenyan Rift Valley at 1,776 m above sea level and has no direct outlet. The microbial diversity of the lake was investigated using a culture-independent approach. Five different sampling points w...

journal_title：Current microbiology

authors： Mwirichia R,Cousin S,Muigai AW,Boga HI,Stackebrandt E

更新日期：2011-01-01 00:00:00

abstract：:Fungal communities in decaying sapwood and heartwood of K. evelyniana were demonstrated through construction of four 18 S rRNA gene libraries. The 210 sequenced clones were clustered into 11 subgroups, belonging to Basidiomycota (71.9%) and to Ascomycota (22.4%) and unclassified (1 subgroup; 5.7%). The heartwood displ...

journal_title：Current microbiology

authors： Zhang HB,Yang MX,Tu R,Gao L,Zhao ZW

更新日期：2008-04-01 00:00:00

abstract：:To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...

journal_title：Current microbiology

authors： Brambila-Tapia AJ,Poot-Hernández AC,Garcia-Guevara JF,Rodríguez-Vázquez K

更新日期：2016-06-01 00:00:00

abstract：:Bats can be divided into frugivory, nectarivory, insectivory, and sanguivory based on their diets, and are therefore ideal wild animal models to study the relationship between diets and intestinal microflora. Early studies of bat gut bacteria showed that the diversity and structure of intestinal bacterial communities ...

journal_title：Current microbiology

authors： Li J,Li L,Jiang H,Yuan L,Zhang L,Ma JE,Zhang X,Cheng M,Chen J

更新日期：2018-10-01 00:00:00

abstract：:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized a...

journal_title：Current microbiology

authors： Karunakaran T,Gunasekaran P

更新日期：1992-11-01 00:00:00

abstract：:In this study, the effect of rhamnolipid biosurfactant produced by Pseudomonas fluorescens on bacterial strains, laboratory strains, and isolates from industrial wastewater was investigated. It was shown that biosurfactant, depending on the concentration, has a neutral or detrimental effect on the growth and protein r...

journal_title：Current microbiology

authors： Vasileva-Tonkova E,Sotirova A,Galabova D

更新日期：2011-02-01 00:00:00

abstract：:Staphylococcus aureus causes a wide range of suppurative infections in humans and animals. Due to its high virulence, ability to adopt various environmental conditions, and acquired multiple drug resistance, treatment of such infections has become difficult. Therefore, there is an immense need to develop alternate dru...

journal_title：Current microbiology

authors： Gupta R,Prasad Y

更新日期：2011-07-01 00:00:00

abstract：:Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...

journal_title：Current microbiology

authors： Guerlava P,Izac V,Tholozan JL

更新日期：1998-03-01 00:00:00

abstract：:Recently, we showed that the southern green stink bug (SGSB), Nezara viridula (L.), can transmit Pantoea agglomerans (Ewing and Fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. Here, we hypothesized that our established model could be used to determine if the SGSB was a general, n...

journal_title：Current microbiology

authors： Medrano EG,Esquivel J,Bell A,Greene J,Roberts P,Bacheler J,Marois J,Wright D,Nichols R,Lopez J

更新日期：2009-10-01 00:00:00

abstract：:A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB...

journal_title：Current microbiology

authors： Leong KH,Chen YS,Pan SF,Chen JJ,Wu HC,Chang YC,Yanagida F

更新日期：2014-04-01 00:00:00

abstract：:Zoospores of Chlamydomonas reinhardtii exhibit chemotaxis towards maltose, sucrose, xylose, mannitol, and ammonium. Ten independent mutants defective in chemotaxis towards sugars have been isolated. These mutants form five phenotypic classes. Genetic analysis of two mutant strains defective in chemotaxis to maltose (C...

journal_title：Current microbiology

authors： Ermilova EV,Chekunova EM,Zalutskaya ZM,Krupnov KR,Gromov BV

更新日期：1996-06-01 00:00:00

abstract：:A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...

journal_title：Current microbiology

authors： Zhang H,Zhao HZ,Liu J,Qin W,Huang X

更新日期：2020-11-01 00:00:00

abstract：:The production and its potential use of a novel trihydroxy unsaturated fatty acid, 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD), were investigated. TOD was formed by Pseudomonas aeruginosa PR3 (NRRL B-18602) in a culture supplied with exogenous ricinoleic acid. The yield of TOD production was always higher in a ric...

journal_title：Current microbiology

authors： Kuo TM,Kim H,Hou CT

更新日期：2001-09-01 00:00:00

abstract：:Moutai Jiuqu is a famous aromatic raw material of Maotai flavor liquor in China. It is brewed at high temperature and contains many kinds of bacteria, molds, and yeasts. There are many useful glycoside hydrolases in these microfloras, from which efficient glycoside hydrolases can be screened for biotransformation of n...

journal_title：Current microbiology

authors： Zuo SS,Wang YC,Zhu L,Zhao JY,Li MG,Han XL,Wen ML

更新日期：2020-10-01 00:00:00

abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...

journal_title：Current microbiology

authors： Chauhan VS,Singh B,Singh S,Bisen PS

更新日期：2001-02-01 00:00:00

abstract：:Polyclonal antibodies against Escherichia coli and fluorescent, secondary, antibodies were immobilized on borosilicate glass fibers pre-treated with 3-glycidyloxypropyl trimethoxysilane (GPS). Light with an average wavelength of 627 nm, emitted by a diode placed at one end of the glass fiber, was detected by an ultras...

journal_title：Current microbiology

authors： Maas MB,Maybery GHC,Perold WJ,Neveling DP,Dicks LMT

更新日期：2018-02-01 00:00:00

abstract：:A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...

journal_title：Current microbiology

authors： Volokita M,Abeliovich A,Soares MI

更新日期：2000-02-01 00:00:00

abstract：:A systematic evaluation of the ability of different bacterial genera to transform 2,4,6-trinitrotoluene (TNT), and grow in its presence, was conducted. Aerobic Gram-negative organisms degraded TNT and evidenced net consumption of reduced metabolites when cultured in molasses medium. Some Gram-negative isolates transfo...

journal_title：Current microbiology

authors： Fuller ME,Manning JF Jr

更新日期：1997-08-01 00:00:00

abstract：:We report the in vitro phytopathogen suppression activity of siderophoregenic preparations of Alcaligenes feacalis vis-à-vis the oraganochlorine fungicide, bavistin. Siderophore-rich culture broth, siderophore-rich supernatant, and purified siderophore preparation exerted antifungal activity against Aspergillus niger ...

journal_title：Current microbiology

authors： Sayyed RZ,Chincholkar SB

更新日期：2009-01-01 00:00:00

abstract：:Fourteen forms of endopolygalacturonases were purified from the culture medium of Sclerotinia sclerotiorum with ion exchange chromatographies. Individual forms differ in their isoelectric point but exhibit similar molecular masses. On the basis of the cross-reactions to antibodies raised against a purified acidic endo...

journal_title：Current microbiology

authors： Martel MB,Letoublon R,Fevre M

更新日期：1996-10-01 00:00:00

abstract：:Pandemic influenza A (H1N1) 2009 virus was first detected in India in May 2009 which subsequently became endemic in many parts of the country. Influenza A viruses have the ability to evade the immune response through its ability of antigenic variations. The study aims to characterize influenza A (H1N1) pdm 09 viruses ...

journal_title：Current microbiology

authors： Gohil D,Kothari S,Shinde P,Meharunkar R,Warke R,Chowdhary A,Deshmukh R

更新日期：2017-08-01 00:00:00

abstract：:In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...

journal_title：Current microbiology

authors： Zeng T,Li D,Zeng H,Zhang Z,Liu L,Zhang X,Zhang J

更新日期：2013-06-01 00:00:00

abstract：:Favre and coworkers (Favre et al., Biochimie 73:235-244, 1991) previously reported that the Bordetella pertussis recA gene present at high copy number could promote a low frequency of recombination, but not bacteriophage induction in Escherichia coli RecA- mutants. Reexamination of these phenotypes demonstrated that, ...

journal_title：Current microbiology

authors： Kuhl SA

更新日期：1996-03-01 00:00:00