Aerobic gram-positive and gram-negative bacteria exhibit differential sensitivity to and transformation of 2,4,6-trinitrotoluene (TNT).

Abstract:

:A systematic evaluation of the ability of different bacterial genera to transform 2,4,6-trinitrotoluene (TNT), and grow in its presence, was conducted. Aerobic Gram-negative organisms degraded TNT and evidenced net consumption of reduced metabolites when cultured in molasses medium. Some Gram-negative isolates transformed all the initial TNT to undetectable metabolites, with no adsorption of TNT or metabolites to cells. Growth and TNT transformation capacity of Gram-positive bacteria both exhibited 50% reductions in the presence of approximately 10 microg TNT ml-1. Most non-sporeforming Gram-positive organisms incubated in molasses media amended with 80 microg TNT ml-1 became unculturable, whereas all strains tested remained culturable when incubated in mineral media amended with 98 microg TNT ml-1, indicating that TNT sensitivity is linked to metabolic activity. These results indicate that the microbial ecology of soil may be severely impacted by TNT contamination.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Fuller ME,Manning JF Jr

doi

10.1007/s002849900216

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

77-83

issue

2

eissn

0343-8651

issn

1432-0991

journal_volume

35

pub_type

杂志文章
  • Development of a Novel Allele-Specific PCR Method for Rapid Assessment of Nervous Necrosis Virus Genotypes.

    abstract::Viral nervous necrosis infections are causing severe problems on aquaculture industry due to ecological and economic impacts. Their causal agent is nervous necrosis virus or nodavirus, which has been classified into four genotypes. Different genotypes correlate with differences in viral pathogenicity. Therefore, ratio...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0880-0

    authors: Toubanaki DK,Margaroni M,Karagouni E

    更新日期:2015-11-01 00:00:00

  • Helicobacter pylori identification: a diagnostic/confirmatory method for evaluation.

    abstract::The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region we...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0578-8

    authors: Mesquita B,Gonçalves MJ,Pacheco P,Lopes J,Salazar F,Relvas M,Coelho C,Pacheco JJ,Velazco C

    更新日期:2014-09-01 00:00:00

  • Cloning, Expression, Purification, Regulation, and Subcellular Localization of a Mini-protein from Campylobacter jejuni.

    abstract::The Cj1169c-encoded putative protein of Campylobacter was expressed and purified from E. coli after sequence optimization. The purified protein allowed the production of a specific rabbit antiserum that was used to study the protein expression in vitro and its subcellular localization in the bacterial cell and putativ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0980-x

    authors: Aliouane S,Pagès JM,Bolla JM

    更新日期:2016-05-01 00:00:00

  • Cloning of Schizosaccharomyces pombe bio2 by heterologous complementation of a Saccharomyces cerevisiae mutant.

    abstract::A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900470

    authors: Phalip V,Lemoine Y,Jeltsch JM

    更新日期:1999-12-01 00:00:00

  • A Preliminary Study on Probiotic Characteristics of Sporosarcina spp. for Poultry Applications.

    abstract::Probiotics are well known for their wide range of beneficial activities. However, recent use of probiotic Bifidobacterium, Enterococcus, and Lactobacillus spp. has been plagued by certain disadvantages such as complex growth requirements, high maintenance cost, susceptibility to the gastrointestinal environment, patho...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01647-2

    authors: Priyodip P,Balaji S

    更新日期:2019-04-01 00:00:00

  • Optimization and Characterization of PHA (SCL-SCL) Copolymer by Indigenous Bacillus thuringiensis A102 Strain for Biomedical Applications.

    abstract::PHA is one of the leading commercially important bio-polyesteric compounds piled up as an intracellular lipid-based energy storage compound by numerous microorganisms. An indigenous Gram-positive bacterium isolated from fire ant (Solenopsis invicta) has known to potentially accumulate PHA. Various nutritional elements...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02221-x

    authors: Suguna PP,Saranya VV,Abirami PP,Shenbagarathai RR

    更新日期:2020-12-01 00:00:00

  • Microbiomes of Biofilms on Decorative Siliceous Stone: Drawbacks and Advantages of Next Generation Sequencing.

    abstract::Next Generation Sequencing (NGS), using the Illumina® metabarcoding system, showed differences between biofilm communities on three degraded siliceous stone church façades in central Rio de Janeiro. Two church biofilms (on granite and augen gneiss) were dominated by Actinobacteria; the third (granite), surrounded by t...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1257-3

    authors: Ogawa A,Celikkol-Aydin S,Gaylarde C,Baptista-Neto JA,Beech I

    更新日期:2017-07-01 00:00:00

  • Biofilm formation by Salmonella enterica serovar 1,4,[5],12:i:- Portuguese isolates: a phenotypic, genotypic, and socio-geographic analysis.

    abstract::Biofilm-forming ability is well established as an important virulence factor. However, there are no studies available regarding biofilm formation of Salmonella Typhimurium 1,4,[5],12:i:-, the new pandemic serovar in Europe. To address this problem, biofilm expression by Salmonella 1,4,[5],12:i:- was evaluated using 13...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0523-x

    authors: Seixas R,Machado J,Bernardo F,Vilela C,Oliveira M

    更新日期:2014-05-01 00:00:00

  • Proteomic analysis of protein expression in the induction of the viable but Nonculturable State of Vibrio harveyi SF1.

    abstract::Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cy...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0383-9

    authors: Jia J,Li Z,Cao J,Jiang Y,Liang C,Liu M

    更新日期:2013-10-01 00:00:00

  • Sequencing, characterization, and gene expression analysis of the histidine decarboxylase gene cluster of Morganella morganii.

    abstract::The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0490-7

    authors: Ferrario C,Borgo F,de Las Rivas B,Muñoz R,Ricci G,Fortina MG

    更新日期:2014-03-01 00:00:00

  • Generation and characterization of thymidine/D-alanine auxotrophic recombinant Lactococcus lactis subsp. lactis IL1403 expressing BmpB.

    abstract::Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9572-y

    authors: Kim EB,Son JS,Zhang QK,Lee NK,Kim SH,Choi JH,Kang SK,Choi YJ

    更新日期:2010-07-01 00:00:00

  • Response of heat-shock protein (HSP) genes to temperature and salinity stress in the antarctic psychrotrophic bacterium Psychrobacter sp. G.

    abstract::Temperature and salinity fluctuations are two of the most important factors affecting the growth of polar bacteria. In an attempt to better understand the function of heat-shock proteins (HSPs) in the adaptive mechanisms of the Antarctic psychrotrophic bacterium Psychrobacter sp. G to such conditions, genes Hsp845, Hs...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0409-3

    authors: Che S,Song W,Lin X

    更新日期:2013-11-01 00:00:00

  • Cys92, Cys101, Cys197, and Cys203 are crucial residues for coordinating the iron-sulfur cluster of RhdA from Acidithiobacillus ferrooxidans.

    abstract::By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9476-x

    authors: Dai Y,Liu J,Zheng C,Wu A,Zeng J,Qiu G

    更新日期:2009-11-01 00:00:00

  • Photonic Potential of Haloarchaeal Pigment Bacteriorhodopsin for Future Electronics: A Review.

    abstract::Haloarchaea are known for its adaptation in extreme saline environment. Halophilic archaea produces carotenoid pigments and proton pumps to protect them from extremes of salinity. Bacteriorhodopsin (bR) is a light-driven proton pump that resides in the membrane of haloarchaea Halobacterium salinarum. The photocycle of...

    journal_title:Current microbiology

    pub_type: 杂志文章,评审

    doi:10.1007/s00284-017-1271-5

    authors: Ashwini R,Vijayanand S,Hemapriya J

    更新日期:2017-08-01 00:00:00

  • Formic acid and acetic acid induce a programmed cell death in pathogenic Candida species.

    abstract::Cutaneous fungal infections are common and widespread. Antifungal agents used for the treatment of these infections often have undesirable side effects. Furthermore, increased resistance of the microorganisms to the antifungal drugs becomes the growing problem. Accordingly, the search for natural antifungal compounds ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0585-9

    authors: Lastauskienė E,Zinkevičienė A,Girkontaitė I,Kaunietis A,Kvedarienė V

    更新日期:2014-09-01 00:00:00

  • Identification and initial characterization of elastase activity associated with Vibrio cholerae.

    abstract::Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900421

    authors: Janda JM,Abbott SL,Khashe S

    更新日期:1999-08-01 00:00:00

  • Analysis of the cbbF genes from Alcaligenes eutrophus that encode fructose-1,6-/sedoheptulose-1,7-bisphosphatase.

    abstract::The cbbF genes of the facultative chemoautotroph Alcaligenes eutrophus H16 are part of two highly homologous cbb operons. Both the chromosomal and the megaplasmid pHG1-borne copy of cbbF were cloned and sequenced. Subsequent analyses including comparison with known sequences from other organisms and heterologous expre...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00294635

    authors: Yoo JG,Bowien B

    更新日期:1995-07-01 00:00:00

  • Differentiation of Nostoc flagelliforme and its neighboring species using fatty-acid profiling as a chemotaxonomic tool.

    abstract::In this study, fatty-acid content and patterns were analyzed in order to distinguish Nostoc flagelliforme, an edible terrestrial cyanobacterium, from other Nostoc species and representatives typical of its close neighbors (genera Anabaena, Microcystis, and Synechococcus). According to the Kenyon-Murata classification ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4059-8

    authors: Liu XJ,Chen F,Jiang Y

    更新日期:2003-12-01 00:00:00

  • Arsenite-induced multiple antibiotic resistance phenotype in environmental isolates of Yersinia enterocolitica.

    abstract::Minimal inhibitory concentrations (MICs) of five antibiotics namely amikacin, gentamicin, tetracycline, ciprofloxacin, and nitrofurantoin for pork isolates of Yersinia enterocolitica increased two- to eightfold after bacteria were grown in the presence of 5 mm arsenite. For Y. enterocolitica isolates obtained from was...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002840010277

    authors: Virdi JS,Sinha I,Rajendran P,Singh I

    更新日期:2001-08-01 00:00:00

  • Diversity of lactic acid bacteria associated with fresh coffee cherries in Taiwan.

    abstract::A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0495-2

    authors: Leong KH,Chen YS,Pan SF,Chen JJ,Wu HC,Chang YC,Yanagida F

    更新日期:2014-04-01 00:00:00

  • Escherichia coli O157 somatic antigen is present in an isolate of E. fergusonii.

    abstract::A bacterium that tested positive with antibodies specific for Escherichia coli O157 was isolated from beef during routine screening procedures. The bacterium was identified as E. fergusonii by biochemical testing and partial sequencing of 16S rRNA. The isolate was tested for the presence of genes encoding Shiga toxins...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-0447-6

    authors: Fegan N,Barlow RS,Gobius KS

    更新日期:2006-06-01 00:00:00

  • Common Mechanism of Cross-Resistance Development in Pathogenic Bacteria Bacillus cereus Against Alamethicin and Pediocin Involves Alteration in Lipid Composition.

    abstract::To understand the mechanism of development of cross-resistance in food pathogen Bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. Pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followe...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-016-1090-0

    authors: Meena S,Mehla J,Kumar R,Sood SK

    更新日期:2016-10-01 00:00:00

  • Phage displayed Bacillus thuringiensis Cry1Ba4 toxin is toxic to Plutella xylostella.

    abstract::We constructed recombinant phage particles displaying the Bacillus thuringiensis Cry1Ba4 active toxin using the pfUSE5 and pComb3X phagemid vectors. The recombinant phage particles were screened and evaluated for displayed biologically active Cry1Ba4 toxin against the target insect larvae. Concurrent expression of Cry...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0164-9

    authors: Nathan S,Aziz DH,Mahadi NM

    更新日期:2006-11-01 00:00:00

  • The luxS gene is involved in AI-2 production, pathogenicity, and some phenotypes in Erwinia amylovora.

    abstract::Erwinia amylovora causes fire blight of apple, pear, and other members of the Rosaceae family. The enzyme LuxS catalyzes the last step in the production of autoinducer-2 (AI-2), a molecule implicated with quorum sensing in many bacterial species. It is now well recognized that LuxS also plays a central role in sulfur ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9256-z

    authors: Gao Y,Song J,Hu B,Zhang L,Liu Q,Liu F

    更新日期:2009-01-01 00:00:00

  • Biodegradation of p-cresol by Bacillus sp. strain PHN 1.

    abstract::A bacterium capable of utilizing p-cresol as sole source of carbon and energy was isolated from soil and identified as a Bacillus species. The organism also utilized phenol, o-cresol, m-cresol, 4-hydroxybenzoic acid, and gentisic acid as growth substrates. The organism degraded p-cresol to 4-hydroxybenzoic acid, which...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0309-x

    authors: Tallur PN,Megadi VB,Kamanavalli CM,Ninnekar HZ

    更新日期:2006-12-01 00:00:00

  • Biofouling of groundwater systems by Thiothrix spp.

    abstract::Thiothrix spp., sulfide-oxidizing filamentous bacteria, were found to be a principal bacterial component of aquatic biofilms causing biofouling in selected municipal water storage tanks, private wells, and drip irrigation systems in Florida. Treatments of up to 200 ppm chlorine in the affected systems could not preven...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900233

    authors: Brigmon RL,Martin HW,Aldrich HC

    更新日期:1997-09-01 00:00:00

  • Enhanced biodegradation of anthracene in acidic soil by inoculated Burkholderia sp. VUN10013.

    abstract::The ability of Burkholderia sp. VUN10013 to degrade anthracene in microcosms of two acidic Thai soils was studied. The addition of Burkholderia sp. VUN10013 (initial concentration of 10(5) cells g(-1) dry soil) to autoclaved soil collected from the Plew District, Chanthaburi Province, Thailand, supplemented with anthr...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9157-1

    authors: Somtrakoon K,Suanjit S,Pokethitiyook P,Kruatrachue M,Lee H,Upatham S

    更新日期:2008-08-01 00:00:00

  • Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea.

    abstract::Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage tite...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0984-6

    authors: Li Y,Wang M,Liu Q,Song X,Wang D,Ma Y,Shao H,Jiang Y

    更新日期:2016-05-01 00:00:00

  • AbrB and Spo0E control the proper timing of sporulation in Bacillus subtilis.

    abstract::We have shown previously that Spo0A approximately P-dependent sinIR operon expression was substantially down-regulated in abrB null mutant backgrounds. In this report, we show that loss of function mutations in abrB also cause phosphorelay gene expression to be down regulated. abrB null mutations caused diminished veg...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4186-2

    authors: Shafikhani SH,Leighton T

    更新日期:2004-04-01 00:00:00

  • Isolation and characterization of new cyclohexylacetic acid-degrading bacteria.

    abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9158-0

    authors: Iwaki H,Nakai E,Nakamura S,Hasegawa Y

    更新日期:2008-08-01 00:00:00