Abstract:
:The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region were performed, and digested with the restriction enzyme DdeI. We observed that the PCR-RFLP approach augments the accuracy from 26.2 % (16/61), found in the PCR-based results, to 42.6 % (26/61), which is an excellent indicator for the establishment of this low-cost procedure as a diagnostic/confirmatory method for H. pylori evaluation.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Mesquita B,Gonçalves MJ,Pacheco P,Lopes J,Salazar F,Relvas M,Coelho C,Pacheco JJ,Velazco Cdoi
10.1007/s00284-014-0578-8subject
Has Abstractpub_date
2014-09-01 00:00:00pages
245-51issue
3eissn
0343-8651issn
1432-0991journal_volume
69pub_type
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