Helicobacter pylori identification: a diagnostic/confirmatory method for evaluation.

Abstract:

:The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region were performed, and digested with the restriction enzyme DdeI. We observed that the PCR-RFLP approach augments the accuracy from 26.2 % (16/61), found in the PCR-based results, to 42.6 % (26/61), which is an excellent indicator for the establishment of this low-cost procedure as a diagnostic/confirmatory method for H. pylori evaluation.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Mesquita B,Gonçalves MJ,Pacheco P,Lopes J,Salazar F,Relvas M,Coelho C,Pacheco JJ,Velazco C

doi

10.1007/s00284-014-0578-8

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

245-51

issue

3

eissn

0343-8651

issn

1432-0991

journal_volume

69

pub_type

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