Abstract:
:Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to several mid-gut membrane proteins in Aedes aegypti larvae including prohibitin, a protein recently identified as a receptor that also mediates entry of dengue virus into Aedes cells. This study confirms the interaction between Cry4B and prohibitin by co-immunoprecipitation analysis and demonstrates colocalization of prohibitin and Cry4B by confocal microscopy. While activated Cry4B toxin showed high larvicidal activity, it was not cytotoxic to two Aedes cell lines, allowing determination of its effect on dengue virus infectivity in the absence of Cry4B-induced cell lysis. Pre-exposure of Aedes cells to Cry4B resulted in a significant reduction in the number of infected cells compared to untreated cells.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Kuadkitkan A,Smith DR,Berry Cdoi
10.1007/s00284-012-0178-4subject
Has Abstractpub_date
2012-10-01 00:00:00pages
446-54issue
4eissn
0343-8651issn
1432-0991journal_volume
65pub_type
杂志文章abstract::This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4491-z
更新日期:2005-07-01 00:00:00
abstract::Microorganisms that colonize plants require a number of hydrolytic enzymes to help degrade the cell wall. The maize endophyte Acremonium zeae was surveyed for production of extracellular enzymes that hydrolyze cellulose and hemicellulose. The most prominent enzyme activity in cell-free culture medium from A. zeae NRRL...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9353-z
更新日期:2009-05-01 00:00:00
abstract::Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900402
更新日期:1998-12-01 00:00:00
abstract::Colonial aggregation of Microcystis plays a key role in bloom formation. Limited studies have been reported about effects of environmental factors on the aggregation of Microcystis. Calcium is an important chemical element in water system. In this study, we investigated the effects of a low- (0.015 g l⁻¹) and a high-c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9762-7
更新日期:2011-02-01 00:00:00
abstract::Biofouling and microbiologically influenced corrosion are processes of material deterioration that originate from the attachment of microorganisms as quickly as the material is immersed in a nonsterile environment. Stainless steels, despite their wide use in different industries and as appliances and implant materials...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0193-4
更新日期:2006-12-01 00:00:00
abstract::A Gram-stain-negative, rod-shaped bacterial strain JS15-10A1T was isolated from oil production water. Its optimum growth was observed at 37 °C, pH 7.0, and 3% (w/v) NaCl. The 16S rRNA gene sequence of strain JS15-10A1T showed the highest similarities with Pseudomonas parafulva CB-1T (97.6%) and P. fulva IAM 1529T (97....
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-019-01798-2
更新日期:2020-04-01 00:00:00
abstract::A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-020-02296-6
更新日期:2021-01-02 00:00:00
abstract::Burkholderia cepacia is an opportunistic pathogen that causes serious pulmonary infections in cystic fibrosis patients. Although several potential virulence factors-a protease, lipase, and two phospholipases C (one hemolytic and one nonhemolytic)-have been identified, only two, the protease and the lipase, have been d...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006793
更新日期:1999-04-01 00:00:00
abstract::The mutation of Agrobacterium radiobacter cells exposed to chrysotile asbestos was examined by the random amplified polymorphic DNA (RAPD) method. Approximately 1.4 kbp of DNA in A. radiobacter, which was not amplified strongly in the cells that were not exposed to asbestos, was amplified in the cells that were expose...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00021058
更新日期:2001-06-01 00:00:00
abstract::Cronobacter sakazakii is now recognized as an opportunistic pathogen and has been implicated in rare but severe cases of necrotizing enterocolitis, meningitis, and sepsis in neonates. The first step in bacterial pathogenesis requires that the organism adheres to host cells surfaces; therefore, agents that inhibit adhe...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0623-7
更新日期:2014-10-01 00:00:00
abstract::His(354) and His(358), two highly conserved histidines in Xenopus laevis (6-4) photolyase [equivalent to His(401) and His(405), in Dunaliella salina (6-4) photolyase], are critical for photoreactivation. They act as a base and an acid, respectively. However, the remaining high repair activity when the pH value is high...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9687-1
更新日期:2011-01-01 00:00:00
abstract::Proteolytic activity and a subtilisin inhibitor (NSI) were detected in Natrialba magadii cells. The proteolytic activity was due to two different proteases: a approximately 90-kDa metallo protease (NMP) produced during exponential growth and a 246-kDa serine protease (NSP) detected in the stationary phase. Both protea...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3861-z
更新日期:2003-05-01 00:00:00
abstract::A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02210-0
更新日期:2020-11-01 00:00:00
abstract::A Gram-positive, yellow pigmented actinobacterium, strain S2-48(T) was isolated from water sample collected from the river Brahmaputra, Assam, India and subjected to a polyphasic taxonomic study. Most of the physiological and biochemical properties, major fatty acids (C(15:0) Anteiso, iso C(16:0) and C(17:0) Anteiso),...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9822-z
更新日期:2011-03-01 00:00:00
abstract::Gut microbiota play a central role in the health of animals. The bacteria that individuals acquire as they age may therefore have a profound effect on their future fitness. Since most birds are capable of flight, they can be widely distributed in and adapted to various ecosystems. Moreover, birds are also challenged b...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02048-6
更新日期:2020-11-01 00:00:00
abstract::Root colonization and diversity of arbuscular mycorrhizal fungi (AMF) were analyzed in plants growing in fly ash pond. Eight species could be separated morphologically, while phylogenetic analyses after PCR amplification of the ITS region followed by RFLP and sequencing revealed seven different AM fungal sequence type...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9974-5
更新日期:2011-09-01 00:00:00
abstract::Lake Elmenteita is one of the alkaline saline lakes within the Kenyan Rift valley. The lake is situated on the floor of the Kenyan Rift Valley at 1,776 m above sea level and has no direct outlet. The microbial diversity of the lake was investigated using a culture-independent approach. Five different sampling points w...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9692-4
更新日期:2011-01-01 00:00:00
abstract::Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900421
更新日期:1999-08-01 00:00:00
abstract::The facultative anaerobe Pantoea sp. strain A-13, isolated from ornithogenic soil of Dewart Island (Frazier Islands), Antarctica, produced glycolipid biosurfactants when grown on n-paraffins or kerosene as the sole source of carbon and energy. Hemolysis of erythrocytes, growth inhibition of Bacillus subtilis, and thin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0345-6
更新日期:2007-02-01 00:00:00
abstract::The growth-inhibitory effect of 23 carbohydrate monoesters synthesized by lipases and proteases were assayed to obtain antibacterial agents that suppress the cell growth of Streptococcus mutans. Among the carbohydrate esters synthesized, galactose and fructose laurates showed the highest growth-inhibitory effect, whil...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010121
更新日期:2000-09-01 00:00:00
abstract::The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0352-7
更新日期:2007-01-01 00:00:00
abstract::This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02212-y
更新日期:2020-12-01 00:00:00
abstract::Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not pr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3976-2
更新日期:2003-09-01 00:00:00
abstract::A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty ac...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01802-9
更新日期:2020-01-01 00:00:00
abstract::The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45 degrees C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37 degrees C. The invertase activity was significantly inhibited by bivalent meta...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910036
更新日期:2000-03-01 00:00:00
abstract::The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0243-z
更新日期:2013-01-01 00:00:00
abstract::We tested the hypothesis that supplementation with protein improves fermentation parameters without damaging the rumen microbial populations of beef cattle grazing Urochloa brizantha cv. Marandu during the dry season. Four rumen-cannulated Nellore bulls (571 ± 31 kg of body weight) were used in a 4 × 4 Latin square de...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01631-w
更新日期:2019-03-01 00:00:00
abstract::A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910027
更新日期:2000-02-01 00:00:00
abstract::To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1013-0
更新日期:2016-06-01 00:00:00
abstract::An in vitro study was conducted to examine the metabolism of histidine (His) by mixed rumen bacteria (B), mixed rumen protozoa (P), and a combination of the two (BP). Rumen microorganisms were collected from fistulated goats fed with lucerne cubes (Medicago sativa) and a concentrate mixture twice a day. Microbial susp...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010170
更新日期:2001-01-01 00:00:00