Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae.

Abstract:

:Microorganisms that colonize plants require a number of hydrolytic enzymes to help degrade the cell wall. The maize endophyte Acremonium zeae was surveyed for production of extracellular enzymes that hydrolyze cellulose and hemicellulose. The most prominent enzyme activity in cell-free culture medium from A. zeae NRRL 6415 was xylanase, with a specific activity of 60 U/mg from cultures grown on crude corn fiber. Zymogram analysis following SDS-PAGE indicated six functional xylanase polypeptides of the following masses: 51, 44, 34, 29, 23, and 20 kDa. Xylosidase (0.39 U/mg), arabinofuranosidase (1.2 U/mg), endoglucanase (2.3 U/mg), cellobiohydrolase (1.3 U/mg), and beta-glucosidase (0.85 U/mg) activities were also detected. Although apparently possessing a full complement of hemicellulolytic activities, cell-free culture supernatants prepared from A. zeae required an exogenously added xylosidase to release more than 90% of the xylose and 80% of the arabinose from corn cob and wheat arabinoxylans. The hydrolytic enzymes from A. zeae may be suitable for application in the bioconversion of lignocellulosic biomass into fermentable sugars.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Bischoff KM,Wicklow DT,Jordan DB,de Rezende ST,Liu S,Hughes SR,Rich JO

doi

10.1007/s00284-008-9353-z

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

499-503

issue

5

eissn

0343-8651

issn

1432-0991

journal_volume

58

pub_type

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