Abstract:
:Enterotoxigenic Escherichia coli causes diarrhea by producing several virulence factors including heat-labile enterotoxin (LT). LT is maximally expressed at 37 degrees C. The histone-like nucleoid structuring protein (H-NS) appears to inhibit LT expression by binding to a downstream regulatory element (DRE) at low temperatures. An hns+ E. coli strain, X7026, carrying an LT-beta-galactosidase translational fusion plasmid (pLT-lac) was shown to be responsive to varying amounts of sodium chloride (NaCl) as well as sucrose or lithium chloride. Maximal responsiveness to the various osmolytes was obtained with cells grown at 37 degrees C under microaerophilic conditions. Temperature-osmotic upshift experiments demonstrate LT expression is thermo-osmoregulated. pLT-lac was tested in an hns strain or its congenic hns+ strain for its response to NaCl. LT expression is elevated in the hns strain regardless of NaCl concentration and retains its osmoresponsiveness. The response of the DRE deletion plasmid (pLT-lacDeltaNC) to NaCl is similar to that of the undeleted plasmid.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Trachman JD,Yasmin Mdoi
10.1007/s00284-004-4282-ykeywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
353-60issue
5eissn
0343-8651issn
1432-0991journal_volume
49pub_type
杂志文章abstract::Bacteria use quorum sensing signalling in various functions, e.g. while forming biofilms, and inhibition of this signalling could be one way to control biofilm formation. The aim of this study was to evaluate the production of signalling molecules and its correlation with the biofilm formation capability of bacteria i...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0627-3
更新日期:2014-11-01 00:00:00
abstract::NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00298381
更新日期:1995-10-01 00:00:00
abstract::Bacterial communities communicate, regulate and coordinate their cooperative activities and physiological process by releasing, sensing and responding to small diffusible signal molecules such as acyl homoserine lactones (AHLs), auto-inducing oligo-peptides (AIPs) and autoinducer-2, a process referred to as Quorum sen...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02183-0
更新日期:2020-11-01 00:00:00
abstract::This paper presents the results of studies on the activity of extra-cellular enzymes in soil-willow vegetation filter soil which is used in the post-treatment of household sewage in an onsite wastewater treatment system located in central Poland. Wastewater is discharged from the detached house by gravity into the ons...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0230-4
更新日期:2012-12-01 00:00:00
abstract::A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9T belonge...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1246-6
更新日期:2017-07-01 00:00:00
abstract::The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0295-z
更新日期:2007-03-01 00:00:00
abstract::In eukaryotes, loss of cytoplasmic ribosomal proteins (RPs) results in a reduced growth rate and other phenotypic defects. The ability to transition from a unicellular budding yeast to a filamentous form is very important for biofilm formation and virulence in Candida albicans. Our recent study found that loss of the ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1019-7
更新日期:2016-06-01 00:00:00
abstract::A genetic fingerprinting analysis of Caribbean isolates of M. avium complex (MAC) from AIDS patients by a Southern blotting technique after Pstl digestion with nonradioactive DNA probes coding for single-copy sequences DT1 and DT6 was performed. In parallel, a selective amplification of a 187-bp fragment within the DT...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900127
更新日期:1996-12-01 00:00:00
abstract::Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...
journal_title:Current microbiology
pub_type: 新闻
doi:10.1007/s002849900261
更新日期:1997-11-01 00:00:00
abstract::A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0495-2
更新日期:2014-04-01 00:00:00
abstract::The Cj1169c-encoded putative protein of Campylobacter was expressed and purified from E. coli after sequence optimization. The purified protein allowed the production of a specific rabbit antiserum that was used to study the protein expression in vitro and its subcellular localization in the bacterial cell and putativ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0980-x
更新日期:2016-05-01 00:00:00
abstract::Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01949-w
更新日期:2020-08-01 00:00:00
abstract::A screen of thermophilic lactic acid bacteria identified Streptococcus thermophilus strain ST110 as the putative producer of a bacteriocin with high level of activity against pediococci. Thermophilin 110 was isolated from culture supernatant after 16 h of growth and partially purified by a chloroform extraction proced...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4540-7
更新日期:2005-09-01 00:00:00
abstract::Insecticidal proteins produced by strains of Bacillus thuringenesis are specific toward target pests. One of the Bt proteins, Cry 1Ac has been used successfully for controlling crop predation by polyphagous pests Helicoverpa armigera. Structurally, Bt proteins consist of three domains; domain I and III are fairly homo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9749-4
更新日期:2011-02-01 00:00:00
abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9333-3
更新日期:2009-05-01 00:00:00
abstract::Bipolaris oryzae is a filamentous ascomycetous fungus that causes brown leaf spot disease in rice. We isolated and characterized BLR2, a gene that encodes a putative blue-light regulator similar to Neurospora crassa white collar-2 (WC-2). The deduced amino acid sequence of the BLR2 showed significant homology to other...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9080-x
更新日期:2008-02-01 00:00:00
abstract::The chromosomal DNA of a variety of legionellae, including clinical, environmental, and plasmid-containing strains, was examined for evidence of Dam-like methylation. It was found that Dam-like methylation was present in all the strains examined regardless of their origin or plasmid content. ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900011
更新日期:1996-02-01 00:00:00
abstract::The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected str...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1428-2
更新日期:2018-05-01 00:00:00
abstract::A Gram-stain-negative, rod-shaped bacterial strain JS15-10A1T was isolated from oil production water. Its optimum growth was observed at 37 °C, pH 7.0, and 3% (w/v) NaCl. The 16S rRNA gene sequence of strain JS15-10A1T showed the highest similarities with Pseudomonas parafulva CB-1T (97.6%) and P. fulva IAM 1529T (97....
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-019-01798-2
更新日期:2020-04-01 00:00:00
abstract::Streptomyces coelicolor is the soil-dwelling bacterium with a complex life cycle and a strong ability to produce plenty of secondary metabolites which are strictly regulated by a variety of regulators. Amino acid alignment shows that the deduced protein of SCO2140 belongs to the family of Leucine-responsive regulatory...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1050-8
更新日期:2016-08-01 00:00:00
abstract::A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9469-9
更新日期:2009-11-01 00:00:00
abstract::Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-014-0651-3
更新日期:2014-11-01 00:00:00
abstract::Biofilm-forming ability is well established as an important virulence factor. However, there are no studies available regarding biofilm formation of Salmonella Typhimurium 1,4,[5],12:i:-, the new pandemic serovar in Europe. To address this problem, biofilm expression by Salmonella 1,4,[5],12:i:- was evaluated using 13...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0523-x
更新日期:2014-05-01 00:00:00
abstract::Pseudomonas fluorescens is a physiologically diverse species of bacteria present in many habitats, which possesses multifunctional traits that provide it with the capability to exhibit biological control activities, promote plant health or cause plant disease. Here, we present the draft genome sequence of the kiwifrui...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01655-2
更新日期:2019-05-01 00:00:00
abstract::Flammulina velutipes is a potentially excellent fungus to study basic mechanisms of basidiomycete mycelium biology. To provide a better understanding of the mechanism of hyphae growth and fruit-body formation, the biological functions of the differentially abundant proteins between the fruiting dikaryon and the non-fr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1164-z
更新日期:2017-01-01 00:00:00
abstract::Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice ve...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01570083
更新日期:1992-07-01 00:00:00
abstract::Colonial aggregation of Microcystis plays a key role in bloom formation. Limited studies have been reported about effects of environmental factors on the aggregation of Microcystis. Calcium is an important chemical element in water system. In this study, we investigated the effects of a low- (0.015 g l⁻¹) and a high-c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9762-7
更新日期:2011-02-01 00:00:00
abstract::Several di-, tri-, and oligopeptides were incubated individually in vitro with rumen fluid from two sheep receiving a mixed grass hay/concentrate diet and with washed cells of Prevotella (formerly Bacteroides) ruminicola M384 and P. ruminicola B(1)4. The rates of breakdown of most peptides were similar in the rumen fl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01576265
更新日期:1993-06-01 00:00:00
abstract::The evolution of the symbiotic association with microbes allowed termites to decompose ingested lignocellulose from plant-derived substrates, including herbivore dung and soil humus. Representatives of the Syntermitinae (Termitidae) range in their feeding habits from wood and litter-feeding to humus-feeding species. H...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1567-0
更新日期:2018-12-01 00:00:00
abstract::Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0095-4
更新日期:2002-08-01 00:00:00