Abstract:
:Insecticidal proteins produced by strains of Bacillus thuringenesis are specific toward target pests. One of the Bt proteins, Cry 1Ac has been used successfully for controlling crop predation by polyphagous pests Helicoverpa armigera. Structurally, Bt proteins consist of three domains; domain I and III are fairly homologous in various Bt proteins while domain II is hypervariable. The hypervariable domain II is believed to be responsible for specificity toward target pest. Successful deployment of Bt proteins requires knowledge of its specificity toward the insect. Various Bt proteins have been characterized for activity against coleopteran pests. Some Bt proteins of class Cry6 have been found to be active against potato weevil. We have evaluated the activity of Cry6B protein (BGSC-4D8) against lucerne weevil, Hypera postica, which is a major pest of forage crop Medicago sativa. Results revealed that the purified Cry6B protein is significantly active against the coleopteran pest with LC₅₀ value 280 ng/μl. The leaves coated with the purified Cry6 toxin were three times less damaged as compared with the negative control.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Sharma A,Kumar S,Bhatnagar RKdoi
10.1007/s00284-010-9749-4subject
Has Abstractpub_date
2011-02-01 00:00:00pages
597-605issue
2eissn
0343-8651issn
1432-0991journal_volume
62pub_type
杂志文章abstract::Soil microcosm experiments were used to investigate the effects on growth and activity of soil microorganisms of an herbicide combination (60% bromoxynil + 3% prosulfuron) frequently used to provide a broad spectrum control of weed species. Culturable aerobic bacteria, fungi, and actinomycetes, the fundamental groups ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0116-4
更新日期:2006-09-01 00:00:00
abstract::Potassium tellurite (K2TeO3) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K2TeO3 resistance in STEC is attributable to the ter gene cluster with terD an indicator of the c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1444-x
更新日期:2018-06-01 00:00:00
abstract::This study investigated the antifungal effects of cement paste containing Paenibacillus polymyxa E681 against Aspergillus niger, a deleterious fungus commonly found in cement buildings and structures. To test the antifungal effects, cement paste containing P. polymyxa E681 was neutralized by CO2 gas, and the fungal gr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0604-x
更新日期:2014-10-01 00:00:00
abstract::The addition of increasing physiological concentrations of ammonia or amino acids had distinct effects on the growth and proteolytic activity of Streptococcus bovis JB1, Prevotella albensis, and Butyrivibrio fibrisolvens DSM3071. The growth of S. bovis and B. fibrisolvens was enhanced by NH(3) and AA, and that of P. a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010074
更新日期:2000-06-01 00:00:00
abstract::The aim of the research was (i) to evaluate white and cream strains of A. bisporus yields when challenged or not with the pathogen L. fungicola, (ii) to identify the more aggressive pathogen isolates, and (iii) to develop a diagrammatic scale of spot symptoms on infected mushrooms. The experiment was carried out using...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02232-8
更新日期:2020-12-01 00:00:00
abstract::(13)CNMR and (1)HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells. This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3944-x
更新日期:2003-07-01 00:00:00
abstract::Enterotoxigenic Escherichia coli causes diarrhea by producing several virulence factors including heat-labile enterotoxin (LT). LT is maximally expressed at 37 degrees C. The histone-like nucleoid structuring protein (H-NS) appears to inhibit LT expression by binding to a downstream regulatory element (DRE) at low tem...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4282-y
更新日期:2004-11-01 00:00:00
abstract::The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9831-y
更新日期:2011-04-01 00:00:00
abstract::The Gram-stain-negative, rod-shaped, aerobic strain, designated YC1039T, was isolated from a seamount northern Mariana Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC1039T was related to the genus Mesoflavibacter and had highest 16S rRNA gene sequence...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1500-6
更新日期:2018-09-01 00:00:00
abstract::A total of 168 Campylobacter strains (154 C. jejuni and 14 C. coli) isolated from human clinical samples and chicken meat were typed using Penner serotyping, randomly amplified polymorphic DNA (RAPD), and pulsed-field gel electrophoresis (PFGE) with four restriction enzymes (Sac II, Sal I, Sma I, Kpn I). The 168 strai...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-4037-6
更新日期:2003-11-01 00:00:00
abstract::NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00298381
更新日期:1995-10-01 00:00:00
abstract::There is a region exhibiting a similarity of amino acid sequence near the carboxyl-terminal segment of each FAD-containing oxidoreductase. In this region, four amino acid residues-Thr, Ala, Gly, and Asp-are highly conserved. To determine the involvement of the four amino acid residues (Thr-469, Ala-476, Gly-478, and A...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3911-6
更新日期:2003-06-01 00:00:00
abstract::Native perennial legume Adesmia bicolor reveals characteristics that are key to securing persistence under grazing. Literature on the diversity and symbiotic effectiveness of indigenous rhizobia-nodulating A. bicolor in central Argentina is limited. The purpose of this study was therefore to determine phenotypic and g...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0260-y
更新日期:2013-02-01 00:00:00
abstract::Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900462
更新日期:1999-11-01 00:00:00
abstract::An endolithic bacterium, strain RSBr-1, was isolated from the inside of a piece of red sandstone from coastal areas of Scotland. RSBr-1 was gram negative, oxidase and catalase positive, and cells were non-motile rods. Sodium was required for growth. The optimum sodium chloride concentration and pH for growth were 4% a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4006-8
更新日期:2003-11-01 00:00:00
abstract::Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and ex...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0114-5
更新日期:2002-09-01 00:00:00
abstract::Pseudomonas aeruginosa is a leading cause of hospital-acquired infections and difficult to treat due to acquired-resistance to multiple antibiotics. A pair of strains, M38100A and M38100B, previously identified from a single clinical isolate of P. aeruginosa was investigated to understand phenotypic and genotypic char...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9570-0
更新日期:2010-07-01 00:00:00
abstract::The connection between soil and microbes plays a critical role in soil health and quality and can be elastic with the application of soil amendments and/or crop rotations. Inappropriate management of soil and application of impermissible levels of fertilizers ruptures the overriding connection between the soil and mic...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02028-w
更新日期:2020-09-01 00:00:00
abstract::The rates of urinary tract infection with multidrug-resistant (MDR) Escherichia coli have dramatically increased and the treatment of these infections with single and double antibiotic combinations became limited or ineffective. The present study aimed at finding effective antibiotic combinations against MDR uropathog...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01871-w
更新日期:2020-05-01 00:00:00
abstract::Genetic engineering of microbes for commercial metabolite production traditionally has sought to alter the levels and/or intrinsic activities of key enzymes in relevant biosynthetic pathway(s). Microorganisms exploit similar strategies for flux control, but also coordinate flux through sets of related pathways by usin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010255
更新日期:2001-07-01 00:00:00
abstract::This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9038-z
更新日期:2008-01-01 00:00:00
abstract::The alphaproteobacterial strain JLT2003(T) was isolated from surface seawater off the coast of Guishan island, Taiwan. The strain was Gram negative, ovoid or coccoid, non-motile and formed pink colonies on marine agar 2216 (MA; DIFCO) medium. The dominant fatty acids were C(18:1)ω7c, cyclo C(19:0)ω8c, and C(16:0). The...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0081-z
更新日期:2012-04-01 00:00:00
abstract::Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010081
更新日期:2000-07-01 00:00:00
abstract::The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. or...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1350-7
更新日期:2018-01-01 00:00:00
abstract::Low-substrate regulated microaerophilic behavior (LSRMB) was observed in 10-54% of the bacteria isolated from several fresh-water lakes or ponds, subsurface soils, activated sludge, and Antarctic dry valley soils. Five Pseudomonas and two Bacillus type species showed LSRMB. A subsurface Pseudomonas jessenii strain was...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010097
更新日期:2000-08-01 00:00:00
abstract::A bacterium designated strain B113, able to degrade benzene, toluene, and ethylbenzene compounds (BTE), was isolated from gasoline-contaminated sediment at a gas station in Geoje, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Acinetobacter. The biodegradati...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9268-8
更新日期:2009-01-01 00:00:00
abstract::A gene argH, encoding argininosuccinate lyase (ASL), has been cloned from a cosmid library of the filamentous cyanobacterium Nostoc sp. strain PCC 73102. The argH open reading frame encodes a protein comprised of 461 amino acids with a calculated molecular mass of 51,349 Da. Protein sequence comparisons reveal signifi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010298
更新日期:2001-10-01 00:00:00
abstract::There is a growing interest in understanding the role of the gut microbiome on productive and meat quality-related traits in livestock species in order to develop new useful tools for improving pig production systems and industry. Faecal samples are analysed as a proxy of gut microbiota and here the selection of suita...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1429-1
更新日期:2018-06-01 00:00:00
abstract::Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted levansucrase (LsdA). A levanase-encoding gene ( lsdB), starting 51 bp downstream of the lsdA gene, was cloned from strain SRT4. The lsdB gene (1605 bp) encodes a protein (calculated molecular mass 58.4 kDa) containing a putative 36-amino-acid s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0044-2
更新日期:2002-07-01 00:00:00
abstract::The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01577379
更新日期:1993-04-01 00:00:00