Abstract:
:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the organism was exposed to red light in the presence of toluidine blue, and the membrane fluidity was monitored spectrofluorimetrically by using the membrane probe trimethylammonium diphenyl hexatriene. The fluidity of the organism's cytoplasmic membrane was found to decrease significantly during lethal photosensitization, and this was accompanied by membrane condensation and vacuolation of the cells. Although changes in membrane fluidity are often attributable to lipid peroxidation, malonaldehyde (a product of lipid peroxidation) was not detectable. The disruption of membrane functions associated with a decreased membrane fluidity may contribute to the bactericidal effect of light-activated toluidine blue.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Bhatti M,MacRobert A,Henderson B,Wilson Mdoi
10.1007/s00284-001-0095-4keywords:
subject
Has Abstractpub_date
2002-08-01 00:00:00pages
118-22issue
2eissn
0343-8651issn
1432-0991journal_volume
45pub_type
杂志文章abstract::The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0295-z
更新日期:2007-03-01 00:00:00
abstract::Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9608-3
更新日期:2010-10-01 00:00:00
abstract::To present an overview of the existing epidemiological evidence regarding the occurrence of tinea gladiatorum in wrestlers and dermatophyte contamination in wrestling halls, five electronic databases including "PubMed," "Scopus," "Google Scholar," "Science Direct," and "Web of Science" were searched from inception to ...
journal_title:Current microbiology
pub_type: 杂志文章,meta分析
doi:10.1007/s00284-019-01816-3
更新日期:2020-04-01 00:00:00
abstract::Minimal inhibitory concentrations (MICs) of five antibiotics namely amikacin, gentamicin, tetracycline, ciprofloxacin, and nitrofurantoin for pork isolates of Yersinia enterocolitica increased two- to eightfold after bacteria were grown in the presence of 5 mm arsenite. For Y. enterocolitica isolates obtained from was...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010277
更新日期:2001-08-01 00:00:00
abstract::Helicobacter pylori infection increases the risk of cardiovascular diseases besides leading to duodenal and gastric peptic ulcerations. H. pylori cysteine-rich protein B (HcpB) is a disulfide-rich repeat protein that belongs to the family of Sel1-like repeat proteins. HcpB contains four pairs of anti-parallel alpha he...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9803-2
更新日期:2011-03-01 00:00:00
abstract::A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3978-0
更新日期:2003-08-01 00:00:00
abstract::A common activity in the global search for useful Cry toxins is the microscopic screening of bacterial colonies for the presence of Bacillus thuringiensis. High-throughput screens require that aliquots from large numbers of colonies be arrayed on a microscopic slide. However, precisely placing a small amount of bacter...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0870-2
更新日期:2015-09-01 00:00:00
abstract::In recent years, Candida albicans infections treatment has become a growing problem because, among others, pathogenic strains are capable to develop resistance to the administered drugs. The elaboration of rapid and accurate method of resistance assessment is an important goal of many studies. They aim to avoid inappr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1039-3
更新日期:2016-08-01 00:00:00
abstract::Debaryomyces nepalensis, a halotolerant food-spoiling yeast could grow in complex (YEPD) medium at different pHs ranging between 3.0 and 11.0 in the absence of salt and at pH 3.0-9.0 in the presence of different concentrations of NaCl and KCl. The specific growth rate of D. nepalensis was not affected by the initial p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9249-y
更新日期:2008-12-01 00:00:00
abstract::Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9347-x
更新日期:2009-04-01 00:00:00
abstract::Pseudomonas aeruginosa is a leading cause of hospital-acquired infections and difficult to treat due to acquired-resistance to multiple antibiotics. A pair of strains, M38100A and M38100B, previously identified from a single clinical isolate of P. aeruginosa was investigated to understand phenotypic and genotypic char...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9570-0
更新日期:2010-07-01 00:00:00
abstract::One hundred fifty-five isolates of Bipolaris sorokiniana of wheat were studied for their morphopathological characterization. These isolates were grouped in five categories--black, brown/dull black, gray cottony growth, dull white/greenish black, and white--on the basis of their growth pattern. The frequency of the bl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-0035-z
更新日期:2007-08-01 00:00:00
abstract::A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900470
更新日期:1999-12-01 00:00:00
abstract::Six cyanobacterial isolates recovered from Polynesian microbial mats, called "kopara," were cultured using laboratory-closed photobioreactors and were shown to produce exopolymers as released and capsular exopolysaccharides (EPS). These polymers have been chemically characterized using colorimetric and elemental assay...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0069-z
更新日期:2005-12-01 00:00:00
abstract::Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0483-6
更新日期:2014-03-01 00:00:00
abstract::Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9262-1
更新日期:2009-01-01 00:00:00
abstract::An autolysis chitinase was purified from the cultural medium of the anaerobic fungus Piromyces communis OTS1 by ammonium sulfate precipitation, affinity chromatography with regenerated chitin, chromato-focusing, gel filtration, and chromato-focusing again. The optimal pH and temperature were 6.0 and 50 degrees C, resp...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900210
更新日期:1997-07-01 00:00:00
abstract::The immunosuppressant leflunomide (LFM) inhibits the growth of cytokine-stimulated proliferation of lymphoid cells in vitro and also inhibits the growth of eukaryotic microorganism, Saccharomyces cerevisiae. As a first step to elucidate the molecular mechanism of LFM action in human, yeast gene which suppresses the an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010267
更新日期:2001-08-01 00:00:00
abstract::A Pseudomonas sp. that may be useful in bioremediation projects was isolated from soil. The strain is of potential value because it reduces selenite to elemental red selenium and is unusual in that it was resistant to high concentrations of both selenate and selenite. Exposure of the strain to 50, 100, and 150 mM sele...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9358-2
更新日期:2009-05-01 00:00:00
abstract::Previously we isolated six actinomycetes strains, 9-4, 10-1, 10-2, 10-3, 10-6, and 21-4, that produce phospholipase D (PLD) with high transphosphatidylation activity. In this study, we identified these strains, and the PLD activities were compared with those of reference strains. 16S rDNA sequences and DNA-DNA hybridi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9551-3
更新日期:2010-05-01 00:00:00
abstract::The first cloning and characterization of the gene katA, encoding the major catalase (KatA), from Xanthomonas is reported. A reverse genetic approach using a synthesized katA-specific DNA probe to screen a X. campestris pv. phaseoli genomic library was employed. A positively hybridizing clone designated pKat29 that co...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3812-8
更新日期:2003-02-01 00:00:00
abstract::A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0495-2
更新日期:2014-04-01 00:00:00
abstract::The evolution of the symbiotic association with microbes allowed termites to decompose ingested lignocellulose from plant-derived substrates, including herbivore dung and soil humus. Representatives of the Syntermitinae (Termitidae) range in their feeding habits from wood and litter-feeding to humus-feeding species. H...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1567-0
更新日期:2018-12-01 00:00:00
abstract::The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0352-7
更新日期:2007-01-01 00:00:00
abstract::Legionella and amebae populations in 16 cooling towers were challenged with three commercially available biocide formulations. The active agents were: a chlorinated phenolic thioether (CPTE), bromo-nitro-propane-diol (BNPD), and bromo-chloro-dimethylhydantoin (BCD, in briquette form). The towers were dosed with these ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00296203
更新日期:1995-03-01 00:00:00
abstract::We develop a method to capture marine bacterial strains at high efficiency to replace the conventional two-step collecting method. Lab-made, Fe3O4 magnetic beads were used to firstly verify the feasibility of capture in artificial seawater, using Bacillus velezensis. Almost 100% of the bacteria could be captured and s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01923-6
更新日期:2020-07-01 00:00:00
abstract::Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this st...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1241-y
更新日期:2017-06-01 00:00:00
abstract::Pasteurella haemolytica (Ph) is the most important cause of the bovine acute fibrinohemorrhagic pneumonia that occurs in market stressed calves after shipment to feedyards. Recent characterization of neuraminidase production by these organisms has shown that all 16 serotypes produce an immunologically similar form of ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900372
更新日期:1998-10-01 00:00:00
abstract::The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01577379
更新日期:1993-04-01 00:00:00
abstract::The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900092
更新日期:1996-09-01 00:00:00