Abstract：
:An autolysis chitinase was purified from the cultural medium of the anaerobic fungus Piromyces communis OTS1 by ammonium sulfate precipitation, affinity chromatography with regenerated chitin, chromato-focusing, gel filtration, and chromato-focusing again. The optimal pH and temperature were 6.0 and 50 degrees C, respectively, for a 20-min assay. The chitinase was stable from pH 6.0 to 8.0, but was unstable at 70 degrees C for 20 min. The molecular mass of chitinase was estimated by SDS-PAGE to be 44.9 kDa, and its pI was 4.4. The enzyme activity, which was of the 'endo' type, was inhibited by Hg2+ and allosamidin. The chitinase hydrolyzes chitin powder and fungal cell walls at a higher rate than an artificial chitin substrate. It can be concluded that extracellular chitinase is similar to cytosolic chitinase, but they are not the same protein.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Sakurada M,Morgavi DP,Komatani K,Tomita Y,Onodera Rdoi
10.1007/s002849900210subject
Has Abstractpub_date
1997-07-01 00:00:00pages
48-51issue
1eissn
0343-8651issn
1432-0991journal_volume
35pub_type
杂志文章abstract：:A 11.2-kb fragment containing the ectABC genes of the biosynthetic pathway of ectoine from the Gram-positive, moderately halophilic bacterium Halobacillus dabanensis D-8(T) was obtained by inverse polymerase chain reaction. Subsequently, the entire ectABC cluster was cloned and analyzed. It revealed that the intergeni...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0396-0
更新日期：2006-09-01 00:00:00
abstract：:The outer membrane protein of Photobacterium damsela (OMP-PD) and the gene encoding for this porin protein were isolated and characterized. The deduced amino acid sequence of the OMP-PD monomer has 338 amino acids and a calculated molecular weight of 36,951 Da. This sequence includes a 22-amino acid signal peptide at ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4111-8
更新日期：2004-03-01 00:00:00
abstract：:Corynebacterium diphtheriae strains expressed variation in hydrophobic characteristics dependent on the method used. Results of single assays are not a reliable representation of C. diphtheriae hydrophobicity. All 12 strains adhered to polystyrene surfaces; three showed spontaneous aggregation (SA) in Trypticase Soy B...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006769
更新日期：1999-01-01 00:00:00
abstract：:Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many species, including pigs, horses and humans. Biofilm formation is essential for pathogenesis, and the ability to resist antibiotic treatment results in difficult-to-treat and persistent infections. However, the ability o...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0574-z
更新日期：2014-09-01 00:00:00
abstract：:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0352-7
更新日期：2007-01-01 00:00:00
abstract：:Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02075-3
更新日期：2020-10-01 00:00:00
abstract：:Pasteurella haemolytica (Ph) is the most important cause of the bovine acute fibrinohemorrhagic pneumonia that occurs in market stressed calves after shipment to feedyards. Recent characterization of neuraminidase production by these organisms has shown that all 16 serotypes produce an immunologically similar form of ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900372
更新日期：1998-10-01 00:00:00
abstract：:The gene organization and nucleotide sequence of the type A and B BoNT-gene clusters in Clostridium botulinum strain NCTC 2916 were studied. The aim was to clarify the organization of genes within C. botulinum type A strains possessing an unexpressed BoNT/B gene. The BoNT/A-gene cluster includes genes encoding BoNT, N...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900299
更新日期：1998-04-01 00:00:00
abstract：:Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and posse...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0717-2
更新日期：2015-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900035
更新日期：1996-04-01 00:00:00
abstract：:In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0192-x
更新日期：2006-06-01 00:00:00
abstract：:NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00298381
更新日期：1995-10-01 00:00:00
abstract：:To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1013-0
更新日期：2016-06-01 00:00:00
abstract：:This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4491-z
更新日期：2005-07-01 00:00:00
abstract：:Lake Elmenteita is one of the alkaline saline lakes within the Kenyan Rift valley. The lake is situated on the floor of the Kenyan Rift Valley at 1,776 m above sea level and has no direct outlet. The microbial diversity of the lake was investigated using a culture-independent approach. Five different sampling points w...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9692-4
更新日期：2011-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0133-8
更新日期：2006-03-01 00:00:00
abstract：:Lamprey was considered to be one of the most basal jawless vertebrate representatives for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. Here we investigated the effect of the gut-derived Aeromonas on the lamprey leukocytes proteome using the label-free liquid chromatography-ta...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1468-2
更新日期：2018-07-01 00:00:00
abstract：:By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9476-x
更新日期：2009-11-01 00:00:00
abstract：:Osmotically sensitive mutants of Neurospora crassa are unable to grow on medium supplemented with 4% NaCl, have altered morphologies and cell-wall compositions, and are resistant to dicarboximide fungicides. Osmotic-1 (os-1) mutants have a unique characteristic of forming protoplasts that grow and divide in specialize...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900193
更新日期：1997-06-01 00:00:00
abstract：:Contribution of host genetic backgrounds in the development of gastric microbiota has not been clearly defined. This study was aimed to characterize the biodiversity, structure and composition of gastric microbiota among twins. A total of four pairs of twins and eight unrelated individuals were enrolled in the study. ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1176-8
更新日期：2017-02-01 00:00:00
abstract：:Adenosine 5'-phosphosulfate (APS) reductase is a key enzyme involved in the pathways of sulfate reduction and sulfide oxidation in the biological sulfur cycle. In this study, the gene of APS reductase from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the soluble protein was purified by ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9375-1
更新日期：2009-06-01 00:00:00
abstract：:Betel vine is an edible creeper used in folk medicine to aid digestion since time immemorial. It is an ideal candidate deemed for the bioprospection of endophytic microorganisms with valuable attributes. This study aimed at the characterization of potential bacteria from fermented betel leaves. We report the presence ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02270-2
更新日期：2021-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3911-6
更新日期：2003-06-01 00:00:00
abstract：:We studied the usefulness of flow cytometry for detection of vancomycin resistance in Enterococcus faecalis by direct binding of commercially available fluorescent vancomycin to cells obtained from culture. The cells were stained with Vancomycin@FL, sonicated and additionally stained with propidium iodide (PI). Regard...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9628-z
更新日期：2010-11-01 00:00:00
abstract：:Lactobacillus casei CRL705 produces a class IIb bacteriocin, lactocin 705, which relies on the complementary action of two components, Lac705alpha and Lac705beta. These peptides exert a bactericidal effect on the indicator strain Lactobacillus plantarum CRL691, with an optimal Lac705alpha/Lac705beta peptide ratio of 1...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3844-0
更新日期：2003-03-01 00:00:00
abstract：:A total, 58 strains of Bacillus thuringiensis were isolated from soils of various regions in Korea. Serological tests showed that B. thuringiensis isolates represented 10 H serotypes, indicating a varied flora of B. thuringiensis. But the H serotypes did not have a significantly uneven distribution, ranging from 1 to ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900363
更新日期：1998-09-01 00:00:00
abstract：:Coral mucus-degrading bacteria were isolated by an enrichment culture procedure. The isolates were able to grow as pure cultures on 10% sterilized mucus in seawater, yielding 10(8) CFU/ml. The isolates, mostly Vibrio strains, were classified by classical and molecular methods. When carbon, nitrogen, and phosphorus com...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9100-5
更新日期：2008-05-01 00:00:00
abstract：:Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01739-z
更新日期：2019-12-01 00:00:00
abstract：:To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900336
更新日期：1998-07-01 00:00:00
abstract：:Methanogenesis from furfural by defined mixed cultures was studied. Under sulfate-reducing conditions, a Desulfovibrio strain was used as the furfural-degrading species producing acetic acid. This sulfate-reducing bacterium (SRB) Desulfovibrio strain B is an incomplete oxidizer, unable to carry out the terminal oxidat...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0010-z
更新日期：2002-06-01 00:00:00
