Abstract:
:Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The marine bacteria of Pseudoalteromonas had wide activity of degrading AHLs as a type of signal molecule associated with quorum sensing. We screened many Pseudoalteromonas strains in large scale to explore genes of quorum quenching enzymes from the China seas by whole-genome sequencing rather than genomic library construction. Nine target strains were obtained and an acylases gene APTM01 from the strain MQS005 belonging to PvdQ type on sub-branch in phylogenetic tree. And the heterogenous host containing the vector with target gene could degrade C10-HSL, C12-HSL and OC12-HSL. The obtained AHL acylase gene would be a candidate quorum quenching gene to apply in some fields. We identified that the strains of Pseudoalteromonas have wide AHL-degrading ability depending on quorum quenching. The strains would be a resource to explore new quorum quenching enzymes.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Pan Y,Wang Y,Yan X,Liu C,Wu B,He X,Liang Ydoi
10.1007/s00284-019-01739-zsubject
Has Abstractpub_date
2019-12-01 00:00:00pages
1387-1397issue
12eissn
0343-8651issn
1432-0991pii
10.1007/s00284-019-01739-zjournal_volume
76pub_type
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