Abstract：
:We develop a method to capture marine bacterial strains at high efficiency to replace the conventional two-step collecting method. Lab-made, Fe3O4 magnetic beads were used to firstly verify the feasibility of capture in artificial seawater, using Bacillus velezensis. Almost 100% of the bacteria could be captured and separated within 10 min. Then, the salinity of capture medium was proved to have the most marked effect on the capture process. After that, the broad application and high efficiency of capture were verified using four different bacterial strains from the Pacific Ocean. Subsequently, through adjusting the salinity, the capture efficiency for Pseudoalteromonas sp. and Halomonas meridiana was increased from 20 to ~ 80% in a seawater system, which was used to simulate the in-situ capture conditions. Finally, mixed strains in seawater were successfully captured, and their genomic DNAs were isolated and analyzed. Bare Fe3O4 magnetic beads were initially applied to capture marine microorganisms and this method is convenient and highly efficient and thus has great potential to replace the conventional two-step method.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Zheng Y,Jian X,Xing XH,Zhang Cdoi
10.1007/s00284-020-01923-6subject
Has Abstractpub_date
2020-07-01 00:00:00pages
1210-1216issue
7eissn
0343-8651issn
1432-0991pii
10.1007/s00284-020-01923-6journal_volume
77pub_type
杂志文章abstract：:Saliva is a complex fluid that possesses many important functions regarding oral health. Many in vitro studies require relatively large quantities of saliva. While natural saliva would be the material of choice, it is difficult to obtain in sufficient quantities and varies in composition. Substitutes mimicking the phy...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9937-x
更新日期：2011-07-01 00:00:00
abstract：:Actinomycetes are a wide group of Gram positive prokaryotes, the soil being their most characteristic habitat, where they play important ecological functions. Their immense biotechnological potential as producers of bioactive molecules of great commercial and industrial interest is exemplified by most antibiotics for ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02030-2
更新日期：2020-09-01 00:00:00
abstract：:A genetic fingerprinting analysis of Caribbean isolates of M. avium complex (MAC) from AIDS patients by a Southern blotting technique after Pstl digestion with nonradioactive DNA probes coding for single-copy sequences DT1 and DT6 was performed. In parallel, a selective amplification of a 187-bp fragment within the DT...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900127
更新日期：1996-12-01 00:00:00
abstract：:A broad-host-range vector that expresses a unique artificial RNA in Pseudomonas putida has been developed. This vector was derived from the plasmid pBBR1MCS and incorporates regulatory regions from the Escherichia coli ribosomal operon, rrnB. These include the promoters P1 and P2, and the terminators T1 and T2. The ge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910017
更新日期：2000-02-01 00:00:00
abstract：:The foodborne pathogen Listeria monocytogenes has the ability to develop biofilm in food-processing environment, which becomes a major concern for the food safety. The biofilm formation is strongly influenced by the availability of nutrients and environmental conditions, and particularly enhanced in poor minimal essen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0125-4
更新日期：2012-07-01 00:00:00
abstract：:The evolution of the symbiotic association with microbes allowed termites to decompose ingested lignocellulose from plant-derived substrates, including herbivore dung and soil humus. Representatives of the Syntermitinae (Termitidae) range in their feeding habits from wood and litter-feeding to humus-feeding species. H...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1567-0
更新日期：2018-12-01 00:00:00
abstract：:Genetic engineering of microbes for commercial metabolite production traditionally has sought to alter the levels and/or intrinsic activities of key enzymes in relevant biosynthetic pathway(s). Microorganisms exploit similar strategies for flux control, but also coordinate flux through sets of related pathways by usin...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010255
更新日期：2001-07-01 00:00:00
abstract：:The cluster of genes encoding components of the progenitor botulinum neurotoxin complex has been mapped and cloned in Clostridium botulinum type G strain ATCC 27322. Determination of the nucleotide sequence of the region has revealed open reading frames encoding nontoxic components of the complex, upstream of the gene...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900240
更新日期：1997-10-01 00:00:00
abstract：:A 11.2-kb fragment containing the ectABC genes of the biosynthetic pathway of ectoine from the Gram-positive, moderately halophilic bacterium Halobacillus dabanensis D-8(T) was obtained by inverse polymerase chain reaction. Subsequently, the entire ectABC cluster was cloned and analyzed. It revealed that the intergeni...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0396-0
更新日期：2006-09-01 00:00:00
abstract：:Insecticidal proteins produced by strains of Bacillus thuringenesis are specific toward target pests. One of the Bt proteins, Cry 1Ac has been used successfully for controlling crop predation by polyphagous pests Helicoverpa armigera. Structurally, Bt proteins consist of three domains; domain I and III are fairly homo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9749-4
更新日期：2011-02-01 00:00:00
abstract：:Termites are global pests and can cause serious damage to buildings, crops, and plantation forests. The symbiotic intestinal flora plays an important role in the digestion of cellulose and nitrogen in the life of termites. Termites and their symbiotic microbes in the gut form a synergistic system. These organism work ...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-018-1502-4
更新日期：2019-06-01 00:00:00
abstract：:The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9643-0
更新日期：2010-12-01 00:00:00
abstract：:Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0133-8
更新日期：2006-03-01 00:00:00
abstract：:The goal of this work was the investigation of correlation between some peculiarities of membrane fatty acids composition, biofilm formation, and motility of dam and/or seqA mutants in Salmonella typhimurium bacterial cells and UV-C radiations. The exposure changed the fatty acids composition of dam and seqA/dam strai...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0858-y
更新日期：2015-10-01 00:00:00
abstract：:We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9179-8
更新日期：2008-08-01 00:00:00
abstract：:A gene argH, encoding argininosuccinate lyase (ASL), has been cloned from a cosmid library of the filamentous cyanobacterium Nostoc sp. strain PCC 73102. The argH open reading frame encodes a protein comprised of 461 amino acids with a calculated molecular mass of 51,349 Da. Protein sequence comparisons reveal signifi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010298
更新日期：2001-10-01 00:00:00
abstract：:Subsurface Wastewater Infiltration System (SWIS) is a sewage ecological treatment technology with low investment, energy consumption, and operating cost. SWIS soil contains a large variety of microorganisms. The metabolic process and production of microorganisms are an important basis for qualitatively describing the ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01830-5
更新日期：2020-02-01 00:00:00
abstract：:The great increase in the abundance and phylogenetic diversity of Flavobacterium spp. within a few hundred meters downstream of the discharge site of the Westerhöfer Bach, a hardwater rivulet, raised the question whether adjacent soil may serve as a reservoir of bacteria not detected in discharge water. To address thi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9323-5
更新日期：2009-05-01 00:00:00
abstract：:The Cj1169c-encoded putative protein of Campylobacter was expressed and purified from E. coli after sequence optimization. The purified protein allowed the production of a specific rabbit antiserum that was used to study the protein expression in vitro and its subcellular localization in the bacterial cell and putativ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0980-x
更新日期：2016-05-01 00:00:00
abstract：:Several di-, tri-, and oligopeptides were incubated individually in vitro with rumen fluid from two sheep receiving a mixed grass hay/concentrate diet and with washed cells of Prevotella (formerly Bacteroides) ruminicola M384 and P. ruminicola B(1)4. The rates of breakdown of most peptides were similar in the rumen fl...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01576265
更新日期：1993-06-01 00:00:00
abstract：:This work compared the inhibition effect of the commercially available mouthwash Corsodyl, containing 0.1% chlorhexidine digluconate, and photodynamic inactivation (PDI) employing methylene blue (MB) with irradiation from a red laser on 24-h biofilms formed by Streptococcus mutans strains on hydroxyapatite surfaces. T...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01901-y
更新日期：2020-06-01 00:00:00
abstract：:In the present study, EMA (ethidium monoazide) treatment was applied to a silty-sand reference soil prior to DNA extraction to enable a differentiation between dead and living cells. For this purpose, a reference soil was spiked with Listeria monocytogenes cells or cell equivalents, respectively. With the purpose of e...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01772-y
更新日期：2019-12-01 00:00:00
abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0977-5
更新日期：2016-04-01 00:00:00
abstract：:Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900462
更新日期：1999-11-01 00:00:00
abstract：:Pathogens have evolved an array of strategies to establish a productive infection. The extracellular proteins secreted by pathogens are one of unique mechanisms to evade the host innate immune response. Many secretory proteins transported by the bacterial secretion systems have been widely investigated in Salmonella. ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02201-1
更新日期：2020-11-01 00:00:00
abstract：:Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0009-z
更新日期：2011-12-01 00:00:00
abstract：:Although neutral mine drainage is the less frequent subject of the interest than acid mine drainage, it can have adverse environmental effects caused mainly by precipitation of dissolved Fe. The aim of the study was to characterize the composition of bacterial population in environment with high concentration of iron ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1472-6
更新日期：2018-08-01 00:00:00
abstract：:A moderately psychrophilic bacterium Corynebacterium paurometabolum MTCC 6841 (gram positive, short rod type) producing extracellular alkaline lipase was isolated from Lake Naukuchiatal, Uttaranchal, India. The bacterium was able to grow within a broad range of pH (5-10). Soyabean oil and olive oil served as the best ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0224-6
更新日期：2006-05-01 00:00:00
abstract：:Burkholderia andropogonis is the causal agent of bacterial leaf stripe in sorghum. Strict import quarantine regulations of numerous countries mandate a need for development of a rapid, reliable, and cost-effective diagnostic technique for the identification of B. andropogonis. Using a primer corresponding to the bacte...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4354-z
更新日期：2005-01-01 00:00:00
abstract：:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01575864
更新日期：1992-11-01 00:00:00
