Cloning and expression in Escherichia coli of an alkaline phosphatase (phoA) gene from Zymomonas mobilis.

Abstract:

:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized at one end of the chromosomal insert on plasmid pZAP1. The Z. mobilis phoA gene was expressed from its own promoter in E. coli, and the enzyme was localized to the periplasmic space. Z. mobilis alkaline phosphatase activity in E. coli was repressed in high-phosphate media and derepressed under a phosphate-limited growth condition. These results suggest that Z. mobilis alkaline phosphatase is subjected to normal regulation in E. coli.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Karunakaran T,Gunasekaran P

doi

10.1007/BF01575864

keywords:

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

291-5

issue

5

eissn

0343-8651

issn

1432-0991

journal_volume

25

pub_type

杂志文章
  • Enhanced Hydrogen Production by Co-cultures of Hydrogenase and Nitrogenase in Escherichia coli.

    abstract::Rhodobacter sphaeroides is a bacterium that can produce hydrogen by interaction with hydrogenase and nitrogenase. We report a hydrogen production system using co-cultivation of hydrogenase in liquid medium and immobilized nitrogenase in Escherichia coli. The recombinant plasmid has been constructed to analyze the effe...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0941-4

    authors: Lee HJ,Sekhon SS,Kim YS,Park JY,Kim YH,Min J

    更新日期:2016-03-01 00:00:00

  • Expression of orf1 from the Bacillus thuringiensis NRD-12 cry2Aa1 operon.

    abstract::The 5' untranslated region and the orf1 sequence from the cry2Aa1 operon from Bacillus thuringiensis subsp. kurstaki NRD-12 were sequenced and compared to that from strain HD-1. The start codon described in HD-1 does not yield in NRD-12 a protein of the expected size of 20 kDa, but a 10-amino acid peptide. A second, h...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/pl00006828

    authors: Delattre D,Rang C,Lecointe N,Royer M,Delécluse A,Moar WJ,Frutos R

    更新日期:1999-07-01 00:00:00

  • Biological and molecular variability of Sarocladium oryzae, the sheath rot pathogen of rice (Oryza sativa L.).

    abstract::Sheath rot disease of rice caused by Sarocladium oryzae (Sawada) (=Acrocylindrium oryzae, Sawada) has become an important production constraint in all rice-growing countries. Pathogenicity, phytotoxic metabolites, and random amplified polymorphic DNA (RAPD) markers were used to assess the level of genetic variability ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-4509-6

    authors: Ayyadurai N,Kirubakaran SI,Srisha S,Sakthivel N

    更新日期:2005-06-01 00:00:00

  • Intracellular proteases of Bacillus thuringiensis subsp. kurstaki and a protease-deficient mutant Btk-q.

    abstract::The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3767-9

    authors: Reddy YC,Venkateswerlu G

    更新日期:2002-12-01 00:00:00

  • Polymerase Chain Reaction Assay for Detection of Stenotrophomonas maltophilia in Cheese Samples Based on the smeT Gene.

    abstract::Stenotrophomonas maltophilia is an emerging opportunistic pathogen linked not only to bacteremia, sepsis, and pneumonia but also to severe chronic enteritis. Persons with the impaired immune system are prone to be infected by S. maltophilia since its pathogenicity seems to be more associated with the host immune syste...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1559-0

    authors: Okuno NT,Freire IR,Segundo RTRS,Silva CR,Marin VA

    更新日期:2018-12-01 00:00:00

  • Identification of AstG1, A LAL family regulator that positively controls ansatrienins production in Streptomyces sp. XZQH13.

    abstract::Ansamycins is a group of type I polyketides characterized by the unique starter unit 3-amino-5-hydroxybenzoic acid. This family of secondary metabolites shows diverse biological activities, well-known members of which include rifamycin, geldanamycin, and maytansine. Previously, we isolated an AHBA synthase gene-positi...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0798-6

    authors: Xie C,Deng JJ,Wang HX

    更新日期:2015-06-01 00:00:00

  • Identification and Analysis of a Novel Gene Cluster Involves in Fe2+ Oxidation in Acidithiobacillus ferrooxidans ATCC 23270, a Typical Biomining Acidophile.

    abstract::Iron-oxidizing Acidithiobacillus spp. are applied worldwide in biomining industry to extract metals from sulfide minerals. They derive energy for survival through Fe2+ oxidation and generate Fe3+ for the dissolution of sulfide minerals. However, molecular mechanisms of their iron oxidation still remain elusive. A nove...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1453-9

    authors: Ai C,Liang Y,Miao B,Chen M,Zeng W,Qiu G

    更新日期:2018-07-01 00:00:00

  • Evaluation of Thirty Microalgal Isolates as Biodiesel Feedstocks Based on Lipid Productivity and Triacylglycerol (TAG) Content.

    abstract::Microalgae are considered feedstock for biodiesel production due to their capability to accumulate triacylglycerols, which have a 99% conversion rate into biodiesel, under certain conditions. This study aims to evaluate thirty native microalgal strains as feedstock for biodiesel production based on their biomass and l...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02340-5

    authors: Andeden EE,Ozturk S,Aslim B

    更新日期:2021-01-21 00:00:00

  • Rhesus monkey TRIM5α has distinct HIV-1 restriction activity among different mammalian cell lines.

    abstract::Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-0009-z

    authors: Gong J,Shen XH,Qiu H,Chen C,Yang RG

    更新日期:2011-12-01 00:00:00

  • Insights into the Draft Genome Sequence of the Kiwifruit-Associated Pathogenic Isolate Pseudomonas fluorescens AHK-1.

    abstract::Pseudomonas fluorescens is a physiologically diverse species of bacteria present in many habitats, which possesses multifunctional traits that provide it with the capability to exhibit biological control activities, promote plant health or cause plant disease. Here, we present the draft genome sequence of the kiwifrui...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01655-2

    authors: Zhang L,Wu Z,Wang X,Tan G,Song J

    更新日期:2019-05-01 00:00:00

  • Cloning and expression of the pediocin operon in Streptococcus thermophilus and other lactic fermentation bacteria.

    abstract::Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900462

    authors: Coderre PE,Somkuti GA

    更新日期:1999-11-01 00:00:00

  • Bacteria associated with butterfly stain of Chilean tepa.

    abstract::Butterfly stain, common in Chilean tepa (Laureliopsis philippiana [Looser] Schodde) trees, appears in cross-section of the stem as a series of partially overlapping orange-brown arcs that in early stages resembles a butterfly. Bacterial isolates from stained tepa wood samples cultured under aerobic and anaerobic condi...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849910016

    authors: McLemore JB,Tainter FH,McElreath SD

    更新日期:2000-02-01 00:00:00

  • Comparison of three methods for epidemiological typing of Campylobacter jejuni and C. coli.

    abstract::A total of 168 Campylobacter strains (154 C. jejuni and 14 C. coli) isolated from human clinical samples and chicken meat were typed using Penner serotyping, randomly amplified polymorphic DNA (RAPD), and pulsed-field gel electrophoresis (PFGE) with four restriction enzymes (Sac II, Sal I, Sma I, Kpn I). The 168 strai...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-4037-6

    authors: Ono K,Kurazono T,Niwa H,Itoh K

    更新日期:2003-11-01 00:00:00

  • Transformation in heterokaryons of Neurospora crassa is nuclear rather than cellular phenomenon.

    abstract::In Neurospora crassa, multinucleate macroconidia are used for genetic transformation. The barrier for such a transformation can be either at the cell membrane level or at the nuclear membrane level. For assessment of these possibilities, a forced heterokaryon (containing two genetically marked nuclei and auxotrophic f...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-001-0004-x

    authors: Dev K,Maheshwari R

    更新日期:2002-05-01 00:00:00

  • Biologic control ability of plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 isolated from milk.

    abstract::A plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 (B-30488) was isolated from cows' milk. Bacterial colonization and growth responses of different plant species after inoculation with B-30488 were evaluated in a controlled environment and in microplot assays. Survival and colonization of B-30488 in the p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0261-9

    authors: DasGupta SM,Khan N,Nautiyal CS

    更新日期:2006-12-01 00:00:00

  • iTRAQ-MS/MS Proteomic Analysis Reveals Differentially Expressed Proteins During Post-harvest Maturation of the White Button Mushroom Agaricus bisporus.

    abstract::Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1225-y

    authors: Chen M,Liao J,Li H,Cai Z,Guo Z,Wach MP,Wang Z

    更新日期:2017-05-01 00:00:00

  • Cloning of lipoxygenase genes from a cyanobacterium, Nostoc punctiforme, and its expression in Eschelichia coli.

    abstract::Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0512-9

    authors: Koeduka T,Kajiwara T,Matsui K

    更新日期:2007-04-01 00:00:00

  • Biogenic amine production by Oenococcus oeni.

    abstract::The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-001-0021-9

    authors: Guerrini S,Mangani S,Granchi L,Vincenzini M

    更新日期:2002-05-01 00:00:00

  • Genotypic characterization and phylogenetic relations of Pseudomonas sp. (Formerly P. stutzeri) OX1.

    abstract::Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-0355-9

    authors: Radice F,Orlandi V,Massa V,Cavalca L,Demarta A,Wood TK,Barbieri P

    更新日期:2006-05-01 00:00:00

  • Proteomic analysis of protein expression in the induction of the viable but Nonculturable State of Vibrio harveyi SF1.

    abstract::Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cy...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0383-9

    authors: Jia J,Li Z,Cao J,Jiang Y,Liang C,Liu M

    更新日期:2013-10-01 00:00:00

  • Horizontal Transfer of Antimicrobial Resistance Determinants Among Enteric Pathogens Through Bacterial Conjugation.

    abstract::Multi-drug resistance and transfer of mobile genetic elements among enteric pathogens is being reported to have increased rapidly. Commensal Escherichia coli was previously known to acquire mobile genetic elements from other genus/species. E. coli is also capable of disseminating these elements containing antimicrobia...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01676-x

    authors: Devanga Ragupathi NK,Muthuirulandi Sethuvel DP,Gajendran R,Anandan S,Walia K,Veeraraghavan B

    更新日期:2019-06-01 00:00:00

  • Growth of halotolerant food spoiling yeast Debaryomyces nepalensis NCYC 3413 under the influence of pH and salt.

    abstract::Debaryomyces nepalensis, a halotolerant food-spoiling yeast could grow in complex (YEPD) medium at different pHs ranging between 3.0 and 11.0 in the absence of salt and at pH 3.0-9.0 in the presence of different concentrations of NaCl and KCl. The specific growth rate of D. nepalensis was not affected by the initial p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9249-y

    authors: Kumar S,Lal P,Gummadi SN

    更新日期:2008-12-01 00:00:00

  • The Effect of the Simultaneous Addition of Molybdenum and Tungsten to the Culture Medium on the Formate Dehydrogenase Activity from Methylobacterium sp. RXM

    abstract::Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM an...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900319

    authors: Gírio FM,Roseiro JC,Silva AI

    更新日期:1998-06-01 00:00:00

  • A comparison of the inhibition of translation and 50S ribosomal subunit formation in Staphylococcus aureus cells by nine different macrolide antibiotics.

    abstract::Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900402

    authors: Champney WS,Tober CL,Burdine R

    更新日期:1998-12-01 00:00:00

  • Cytotoxic activity of fungal metabolites from the pathogenic fungus Beauveria bassiana: an intraspecific evaluation of beauvericin production.

    abstract::The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-9977-2

    authors: Valencia JW,Gaitán Bustamante AL,Jiménez AV,Grossi-de-Sá MF

    更新日期:2011-09-01 00:00:00

  • Analysis of the Endophytic Bacteria Community Structure and Function of Panax notoginseng Based on High-Throughput Sequencing.

    abstract::Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02068-2

    authors: Zhang C,Ma X,Zhu R,Liu Z,Gu M,Zhang J,Li Y,Xu Y,Zhu D

    更新日期:2020-10-01 00:00:00

  • Cloning of Schizosaccharomyces pombe bio2 by heterologous complementation of a Saccharomyces cerevisiae mutant.

    abstract::A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900470

    authors: Phalip V,Lemoine Y,Jeltsch JM

    更新日期:1999-12-01 00:00:00

  • Rhodomyrtus tomentosa Leaf Extract and Rhodomyrtone Combat Streptococcus pneumoniae Biofilm and Inhibit Invasiveness to Human Lung Epithelial and Enhance Pneumococcal Phagocytosis by Macrophage.

    abstract::Rhodomyrtus tomentosa leaf has been traditionally used to treat many infections. This plant species has been documented to possess a wide spectrum of pharmacological effects. This study aimed to determine the effects of Rhodomyrtus tomentosa leaf extract and its potent purified compound, rhodomyrtone, on Streptococcus...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02164-3

    authors: Mitsuwan W,Wintachai P,Voravuthikunchai SP

    更新日期:2020-11-01 00:00:00

  • pH and Not Cell Morphology Modulate pLIP2 Induction in the Dimorphic Yeast Yarrowia lipolytica.

    abstract::The dimorphic yeast Yarrowia lipolytica has become an emerging cell factory for recombinant proteins production. Expression vectors involving LIP2 promoter (pLIP2) have been developed and used successfully. However, the relationship between dimorphic transition (i.e., cell morphology) and pLIP2 regulation is still unc...

    journal_title:Current microbiology

    pub_type: 信件

    doi:10.1007/s00284-017-1207-0

    authors: Sassi H,Delvigne F,Kallel H,Fickers P

    更新日期:2017-03-01 00:00:00

  • Segregational stability and copy number of the theta-type lactococcal replicon Rep22 in Lactococcus.

    abstract::Rep22 is the replication region of the lactococcal theta replicating pUCL22 plasmid. The copy number of Rep22-based plasmids in Lactococcus was determined by using a chromosomal DNA fragment from Lactococcus lactis subsp. lactis MMS368 as reference. Segregational behavior appeared to be linked to copy number and there...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00294521

    authors: Frère J,Herreman C,Boutibonnes P,Novel M,Novel G

    更新日期:1995-01-01 00:00:00