Abstract:
:We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescence values per particle in penicillin-resistant than in penicillin-sensitive strains of both species observed. The method allows rapid detection of penicillin resistance in S. aureus and E. faecalis. The results encourage further investigations on the detection of antibiotic resistance in bacteria using flow cytometry.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Jarzembowski T,Wiśniewska K,Józwik A,Bryl E,Witkowski Jdoi
10.1007/s00284-008-9179-8subject
Has Abstractpub_date
2008-08-01 00:00:00pages
167-9issue
2eissn
0343-8651issn
1432-0991journal_volume
57pub_type
杂志文章abstract::We have shown previously that Spo0A approximately P-dependent sinIR operon expression was substantially down-regulated in abrB null mutant backgrounds. In this report, we show that loss of function mutations in abrB also cause phosphorelay gene expression to be down regulated. abrB null mutations caused diminished veg...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4186-2
更新日期:2004-04-01 00:00:00
abstract::A bacterium that tested positive with antibodies specific for Escherichia coli O157 was isolated from beef during routine screening procedures. The bacterium was identified as E. fergusonii by biochemical testing and partial sequencing of 16S rRNA. The isolate was tested for the presence of genes encoding Shiga toxins...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0447-6
更新日期:2006-06-01 00:00:00
abstract::The 5' untranslated region and the orf1 sequence from the cry2Aa1 operon from Bacillus thuringiensis subsp. kurstaki NRD-12 were sequenced and compared to that from strain HD-1. The start codon described in HD-1 does not yield in NRD-12 a protein of the expected size of 20 kDa, but a 10-amino acid peptide. A second, h...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006828
更新日期:1999-07-01 00:00:00
abstract::Stenotrophomonas maltophilia is an emerging opportunistic pathogen linked not only to bacteremia, sepsis, and pneumonia but also to severe chronic enteritis. Persons with the impaired immune system are prone to be infected by S. maltophilia since its pathogenicity seems to be more associated with the host immune syste...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1559-0
更新日期:2018-12-01 00:00:00
abstract::Corynebacterium glutamicum, a Gram-positive soil bacterium belonging to the mycolic acids-containing actinomycetes, is able to use the lignin degradation products ferulate, vanillate, and protocatechuate as sole carbon sources. The gene cluster responsible for vanillate catabolism was identified and characterized. The...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4531-8
更新日期:2005-07-01 00:00:00
abstract::Mycoplasma penetrans invades HeLa cells and survives within them for prolonged periods of time. The intracellular distribution of M. penetrans within HeLa cells was studied utilizing the acidotropic dye LysoTracker (green), which permeates cell membranes and upon protonation remains trapped in acidic compartments. The...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4298-3
更新日期:2004-08-01 00:00:00
abstract::To optimize the cell lysis step for DNA extraction from activated sludge samples, two floc dispersion methods (sonication versus stirring with a cation exchange resin), and three cell lysis treatments (lysozyme + SDS, sonication in a water bath, and thermal shock) were tested. For dispersion, stirring with cation exch...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006809
更新日期:1999-06-01 00:00:00
abstract::The archaeal diversity in salt pan sediment from Mumbai, India, was investigated by 16S rDNA-dependent molecular phylogeny. Small-subunit rRNA (16S rDNA) from salt pan sediment metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain archaea. Thirty-two unique phylotypes were o...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9167-z
更新日期:2008-08-01 00:00:00
abstract::With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8-78.7% identical to that of Cry1B group. The nomenclature assignmen...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910046
更新日期:2000-04-01 00:00:00
abstract::A 11.2-kb fragment containing the ectABC genes of the biosynthetic pathway of ectoine from the Gram-positive, moderately halophilic bacterium Halobacillus dabanensis D-8(T) was obtained by inverse polymerase chain reaction. Subsequently, the entire ectABC cluster was cloned and analyzed. It revealed that the intergeni...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0396-0
更新日期:2006-09-01 00:00:00
abstract::Iturin A is a very important cyclic lipopeptide produced by several B. subtilis strains and has large commercial and therapeutic application potentials but its production on industrial scale has not been realized yet. In the present study, we have observed that the strain ET-1 of Bacillus subtilis, a producer of Ituri...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01764-y
更新日期:2019-12-01 00:00:00
abstract::Microalgae are considered feedstock for biodiesel production due to their capability to accumulate triacylglycerols, which have a 99% conversion rate into biodiesel, under certain conditions. This study aims to evaluate thirty native microalgal strains as feedstock for biodiesel production based on their biomass and l...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02340-5
更新日期:2021-01-21 00:00:00
abstract::A novel isolate of a bacterium, capable of degrading trichloroethylene (TCE) and growing on this as the sole carbon source is reported. The test strain was isolated by an enrichment technique with trichloroethylene as the substrate. The isolated strain belongs to the genus Bacillus. The practical utility of cleaning u...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9427-6
更新日期:2009-09-01 00:00:00
abstract::Biofilm-forming ability is well established as an important virulence factor. However, there are no studies available regarding biofilm formation of Salmonella Typhimurium 1,4,[5],12:i:-, the new pandemic serovar in Europe. To address this problem, biofilm expression by Salmonella 1,4,[5],12:i:- was evaluated using 13...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0523-x
更新日期:2014-05-01 00:00:00
abstract::In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9832-x
更新日期:2011-04-01 00:00:00
abstract::To present an overview of the existing epidemiological evidence regarding the occurrence of tinea gladiatorum in wrestlers and dermatophyte contamination in wrestling halls, five electronic databases including "PubMed," "Scopus," "Google Scholar," "Science Direct," and "Web of Science" were searched from inception to ...
journal_title:Current microbiology
pub_type: 杂志文章,meta分析
doi:10.1007/s00284-019-01816-3
更新日期:2020-04-01 00:00:00
abstract::The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1402-z
更新日期:2018-04-01 00:00:00
abstract::Erwinia amylovora causes fire blight of apple, pear, and other members of the Rosaceae family. The enzyme LuxS catalyzes the last step in the production of autoinducer-2 (AI-2), a molecule implicated with quorum sensing in many bacterial species. It is now well recognized that LuxS also plays a central role in sulfur ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9256-z
更新日期:2009-01-01 00:00:00
abstract::The laccase gene lacD, cloned from a novel laccase-producing basidiomycete Trametes sp. 420, contained 2,052 base pairs (bp) interrupted by 8 introns. lacD displayed a relatively high homology with laccase genes from other white rot fungi, whereas the homology between lacD and laccase genes from plants, insects, or ba...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0068-8
更新日期:2007-04-01 00:00:00
abstract::Biofouling and microbiologically influenced corrosion are processes of material deterioration that originate from the attachment of microorganisms as quickly as the material is immersed in a nonsterile environment. Stainless steels, despite their wide use in different industries and as appliances and implant materials...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0193-4
更新日期:2006-12-01 00:00:00
abstract::The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF02601668
更新日期:1978-01-01 00:00:00
abstract::Cyanide has been proved to be degraded by Klebsiella oxytoca. In order to examine the physiological responses of cyanide degradation by this bacterium, two-dimensional (2-DE) electrophoresis approach and MALDI-TOF-MS allow us to identify 106 proteins spots that were significantly altered in the presence of 1 mM cyanid...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9529-1
更新日期:2010-03-01 00:00:00
abstract::GABA accumulation and glutamate decarboxylase (GAD) activity, the principal enzyme involved in GABA formation, was investigated in cyanobacterium Synechocystis sp. PCC 6803 wild-type (WT) and gad knockout mutant strains grown in medium containing different nitrogenous compounds. Nitrate was the best nitrogen source fo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0687-4
更新日期:2015-01-01 00:00:00
abstract::Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...
journal_title:Current microbiology
pub_type: 新闻
doi:10.1007/s002849900261
更新日期:1997-11-01 00:00:00
abstract::The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0243-z
更新日期:2013-01-01 00:00:00
abstract::In Neurospora crassa, multinucleate macroconidia are used for genetic transformation. The barrier for such a transformation can be either at the cell membrane level or at the nuclear membrane level. For assessment of these possibilities, a forced heterokaryon (containing two genetically marked nuclei and auxotrophic f...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0004-x
更新日期:2002-05-01 00:00:00
abstract::The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment sam...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0056-5
更新日期:2012-03-01 00:00:00
abstract::Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice ve...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01570083
更新日期:1992-07-01 00:00:00
abstract::To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900336
更新日期:1998-07-01 00:00:00
abstract::A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella and Spodoptera exigua was isolated from a Korean soil sample and characterized. The isolate, named B. thuringiensis K1, was determined to belong to ssp. kurstaki (H3a3b3c) type by an H antisera agglutination test and prod...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3755-0
更新日期:2002-10-01 00:00:00