Flow cytometry as a rapid test for detection of penicillin resistance directly in bacterial cells in Enterococcus faecalis and Staphylococcus aureus.

Abstract:

:We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescence values per particle in penicillin-resistant than in penicillin-sensitive strains of both species observed. The method allows rapid detection of penicillin resistance in S. aureus and E. faecalis. The results encourage further investigations on the detection of antibiotic resistance in bacteria using flow cytometry.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Jarzembowski T,Wiśniewska K,Józwik A,Bryl E,Witkowski J

doi

10.1007/s00284-008-9179-8

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

167-9

issue

2

eissn

0343-8651

issn

1432-0991

journal_volume

57

pub_type

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