Isolation and characterization of a strain of Bacillus thuringiensis ssp. kurstaki containing a new delta-endotoxin gene.

abstract：:Polyclonal antibodies against Escherichia coli and fluorescent, secondary, antibodies were immobilized on borosilicate glass fibers pre-treated with 3-glycidyloxypropyl trimethoxysilane (GPS). Light with an average wavelength of 627 nm, emitted by a diode placed at one end of the glass fiber, was detected by an ultras...

journal_title：Current microbiology

authors： Maas MB,Maybery GHC,Perold WJ,Neveling DP,Dicks LMT

更新日期：2018-02-01 00:00:00

abstract：:Native perennial legume Adesmia bicolor reveals characteristics that are key to securing persistence under grazing. Literature on the diversity and symbiotic effectiveness of indigenous rhizobia-nodulating A. bicolor in central Argentina is limited. The purpose of this study was therefore to determine phenotypic and g...

journal_title：Current microbiology

authors： Bianco L,Angelini J,Fabra A,Malpassi R

更新日期：2013-02-01 00:00:00

abstract：:To determine the effect of organics (yeast extract) on microbial community during chalcopyrite bioleaching at different temperature, real-time polymerase chain reaction (PCR) was employed to analyze community dynamics of major bacteria applied in bioleaching. The results showed that yeast extract exerted great impact ...

journal_title：Current microbiology

authors： Li Q,Tian Y,Fu X,Yin H,Zhou Z,Liang Y,Qiu G,Liu J,Liu H,Liang Y,Shen L,Cong J,Liu X

更新日期：2011-08-01 00:00:00

abstract：:To optimize the cell lysis step for DNA extraction from activated sludge samples, two floc dispersion methods (sonication versus stirring with a cation exchange resin), and three cell lysis treatments (lysozyme + SDS, sonication in a water bath, and thermal shock) were tested. For dispersion, stirring with cation exch...

journal_title：Current microbiology

authors： Bourrain M,Achouak W,Urbain V,Heulin T

更新日期：1999-06-01 00:00:00

abstract：:Culture-based analysis was employed in parallel with PCR amplification of 16S rDNA, coupled with denaturing gradient gel electrophoresis (DGGE), to profile bacterial species associated with different developmental stages of the pine false webworm (PFW), Acantholyda erythrocephala, a sawfly pest responsible for inciden...

journal_title：Current microbiology

authors： Zahner V,Lucarotti CJ,McIntosh D

更新日期：2008-12-01 00:00:00

abstract：:Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...

journal_title：Current microbiology

authors： Narayan KD,Pandey SK,Das SK

更新日期：2010-10-01 00:00:00

abstract：:Porcine parvovirus (PPV) can cause reproductive failure in swine, resulting in economic losses to the industry. Antiviral effects of diammonium glycyrrhizinate (DG) have been reported on several animal viruses; however, to date it has yet to be tested on PPV. In this study, the antiviral activity of DG on swine testis...

journal_title：Current microbiology

authors： Li P,Zou H,Ren Y,Zarlenga DS,Ren X

更新日期：2014-07-01 00:00:00

abstract：:Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...

journal_title：Current microbiology

authors： Yu X,Liu T,Sun Z,Guan P,Zhu J,Wang S,Li S,Deng Q,Wang L,Zheng A,Li P

更新日期：2012-04-01 00:00:00

abstract：:A gene encoding the rice (Oryza sativa L.) 90-kDa heat shock protein (OsHsp90) was introduced into Escherichia coli using the pGEX-6p-3 expression vector with a glutathione-S-transferase (GST) tag to analyze the possible function of this protein under heat stress for the first time. We compared the survivability of E....

journal_title：Current microbiology

authors： Liu D,Lu Z,Mao Z,Liu S

更新日期：2009-02-01 00:00:00

abstract：:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...

journal_title：Current microbiology

authors： Pooler MR,Hartung JS

更新日期：1995-12-01 00:00:00

abstract：:Fragments of Synechocystis-DNA driving salt-induced gene expression in E. coli were isolated with translational fusions to a 'lacZ gene. One fragment (fragment 19) showed a NaCl-dependent activation of betaGal expression with the maximum of a ninefold increase in enzyme activity. A similar induction was triggered by t...

journal_title：Current microbiology

authors： Milkowski C,Quinones A,Hagemann M

更新日期：1998-08-01 00:00:00

abstract：:A moderately psychrophilic bacterium Corynebacterium paurometabolum MTCC 6841 (gram positive, short rod type) producing extracellular alkaline lipase was isolated from Lake Naukuchiatal, Uttaranchal, India. The bacterium was able to grow within a broad range of pH (5-10). Soyabean oil and olive oil served as the best ...

journal_title：Current microbiology

authors： Joshi GK,Kumar S,Tripathi BN,Sharma V

更新日期：2006-05-01 00:00:00

abstract：:Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli....

journal_title：Current microbiology

authors： Specht KM,Sheetz KH,Alexander CM,Lamech LT,O'Connor LH,Walker DM,Stevenson HP

更新日期：2010-04-01 00:00:00

abstract：:The 5' untranslated region and the orf1 sequence from the cry2Aa1 operon from Bacillus thuringiensis subsp. kurstaki NRD-12 were sequenced and compared to that from strain HD-1. The start codon described in HD-1 does not yield in NRD-12 a protein of the expected size of 20 kDa, but a 10-amino acid peptide. A second, h...

journal_title：Current microbiology

authors： Delattre D,Rang C,Lecointe N,Royer M,Delécluse A,Moar WJ,Frutos R

更新日期：1999-07-01 00:00:00

abstract：:Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information...

journal_title：Current microbiology

authors： Koeduka T,Kajiwara T,Matsui K

更新日期：2007-04-01 00:00:00

abstract：:Marine subsurface sediments represent a novel archaeal biosphere with unknown physiology. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of active archaea in a sediment core were ch...

journal_title：Current microbiology

authors： Chen J,Wang F,Jiang L,Yin X,Xiao X

更新日期：2013-07-01 00:00:00

abstract：:There is a growing interest in understanding the role of the gut microbiome on productive and meat quality-related traits in livestock species in order to develop new useful tools for improving pig production systems and industry. Faecal samples are analysed as a proxy of gut microbiota and here the selection of suita...

journal_title：Current microbiology

authors： Muiños-Bühl A,González-Recio O,Muñoz M,Óvilo C,García-Casco J,Fernández AI

更新日期：2018-06-01 00:00:00

abstract：:Predation by Bdellovibrio-like organisms (BLOs) results in bacterial community succession in aquatic ecosystems. The effects of nutrient loading on the distribution and phylogeny of BLOs remain largely unknown. To this end, we present our findings on BLO diversity from four north-Indian lakes that are variable in thei...

journal_title：Current microbiology

authors： Chauhan A,Fortenberry GZ,Lewis DE,Williams HN

更新日期：2009-07-01 00:00:00

abstract：:A total of 216 bacterial strains were isolated from rice rhizospheric soils in Northern Thailand. The bacterial strains were initially tested for solubilization of inorganic phosphate, indole acetic acid (IAA) production, selected strains were then tested for optimized conditions for IAA production and whether these c...

journal_title：Current microbiology

authors： Chaiharn M,Lumyong S

更新日期：2011-01-01 00:00:00

abstract：:Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...

journal_title：Current microbiology

authors： Gong J,Shen XH,Qiu H,Chen C,Yang RG

更新日期：2011-12-01 00:00:00

abstract：:Campylobacter jejuni is a major food-borne pathogen that causes human gastroenteritis in many developed countries. In our study, we applied multilocus sequence typing (MLST) technology to 167 C. jejuni isolates from diverse sources in Eastern China to examine their genetic diversity. MLST defined 94 sequence types (ST...

journal_title：Current microbiology

authors： Zhang G,Zhang X,Hu Y,Jiao XA,Huang J

更新日期：2015-09-01 00:00:00

abstract：:Seventy-eight bacterial isolates from human, animal, and plant hosts, representing eight species of the family Enterobacteriaceae, were screened for F-like plasmid sequences. Of the examined human Escherichia coli strains, 28% harbored one or two of the three F-like, RepFI replication regions, while 35% of the examine...

journal_title：Current microbiology

authors： Mulec J,Starcic M,Zgur-Bertok D

更新日期：2002-04-01 00:00:00

abstract：:High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation ti...

journal_title：Current microbiology

authors： Puri S,Beg QK,Gupta R

更新日期：2002-04-01 00:00:00

abstract：:Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...

journal_title：Current microbiology

authors： Yokoyama E,Hirai S,Ishige T,Murakami S

更新日期：2017-04-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis (SE) infection in humans is often associated with the consumption of contaminated poultry products. Binding of the bacterium to the intestinal mucosa is a major pathogenic mechanism of Salmonella in poultry. Transposon mutagenesis identified flgC as a potential binding mutant of...

journal_title：Current microbiology

authors： Shippy DC,Eakley NM,Mikheil DM,Fadl AA

更新日期：2014-05-01 00:00:00

abstract：:Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) an...

journal_title：Current microbiology

authors： Pati S,Maity S,Dash A,Jema S,Mohapatra S,Das S,Samantaray DP

更新日期：2020-07-01 00:00:00

abstract：:A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...

journal_title：Current microbiology

authors： Phalip V,Lemoine Y,Jeltsch JM

更新日期：1999-12-01 00:00:00

abstract：:Coral mucus-degrading bacteria were isolated by an enrichment culture procedure. The isolates were able to grow as pure cultures on 10% sterilized mucus in seawater, yielding 10(8) CFU/ml. The isolates, mostly Vibrio strains, were classified by classical and molecular methods. When carbon, nitrogen, and phosphorus com...

journal_title：Current microbiology

authors： Sharon G,Rosenberg E

更新日期：2008-05-01 00:00:00

abstract：:In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...

journal_title：Current microbiology

authors： Zeng T,Li D,Zeng H,Zhang Z,Liu L,Zhang X,Zhang J

更新日期：2013-06-01 00:00:00

abstract：:Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...

journal_title：Current microbiology

authors： Kim EB,Son JS,Zhang QK,Lee NK,Kim SH,Choi JH,Kang SK,Choi YJ

更新日期：2010-07-01 00:00:00