Evaluating Protocols for Porcine Faecal Microbiome Recollection, Storage and DNA Extraction: from the Farm to the Lab.

abstract：:Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgr1p depletion on cell growth. Tagging Cgr1p with the gre...

journal_title：Current microbiology

authors： Sun J,McFarland M,Boettner D,Panepinto J,Rhodes JC,Askew DS

更新日期：2001-01-01 00:00:00

abstract：:Root colonization and diversity of arbuscular mycorrhizal fungi (AMF) were analyzed in plants growing in fly ash pond. Eight species could be separated morphologically, while phylogenetic analyses after PCR amplification of the ITS region followed by RFLP and sequencing revealed seven different AM fungal sequence type...

journal_title：Current microbiology

authors： Giridhar Babu A,Sudhakara Reddy M

更新日期：2011-09-01 00:00:00

abstract：:A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...

journal_title：Current microbiology

authors： Volokita M,Abeliovich A,Soares MI

更新日期：2000-02-01 00:00:00

abstract：:The goal of this work was the investigation of correlation between some peculiarities of membrane fatty acids composition, biofilm formation, and motility of dam and/or seqA mutants in Salmonella typhimurium bacterial cells and UV-C radiations. The exposure changed the fatty acids composition of dam and seqA/dam strai...

journal_title：Current microbiology

authors： Abdelwaheb C,Lobna M,Bouchra BA,Selma K,Ahmed L

更新日期：2015-10-01 00:00:00

abstract：:Pectin is a diverse polysaccharide comprising of polygalacturonic acid, rhamnogalactouronan, and neutral polysaccharides (arabinan and arabinogalactan) as side chains or branches. They are resistant to salivary amylase and gastric juice. In the present study the prebiotic potentials of different pectic derived substra...

journal_title：Current microbiology

authors： Tingirikari JMR

更新日期：2019-12-01 00:00:00

abstract：:Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...

journal_title：Current microbiology

authors： Janda JM,Abbott SL,Khashe S

更新日期：1999-08-01 00:00:00

abstract：:DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) is one of the pesticides that are hazardous for the environment and human health. Effective environmental-friendly treatment using co-cultures of fungi and bacteria is needed. In this study, the bacteria Bacillus subtilis at various volumes of 1, 3, 5, 7, and 10 mL (...

journal_title：Current microbiology

authors： Sariwati A,Purnomo AS,Kamei I

更新日期：2017-09-01 00:00:00

abstract：:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...

journal_title：Current microbiology

authors： Zheng D,Valdez-Cruz NA,Armengol G,Sevrez C,Munoz-Olaya JM,Yuan Z,Orduz S,Crickmore N

更新日期：2007-01-01 00:00:00

abstract：:A systematic evaluation of the ability of different bacterial genera to transform 2,4,6-trinitrotoluene (TNT), and grow in its presence, was conducted. Aerobic Gram-negative organisms degraded TNT and evidenced net consumption of reduced metabolites when cultured in molasses medium. Some Gram-negative isolates transfo...

journal_title：Current microbiology

authors： Fuller ME,Manning JF Jr

更新日期：1997-08-01 00:00:00

abstract：:White-rot fungal strains of Trametes versicolor and Phanerochaete chrysosporium were selected to study the decolourisation of the textile dye, Reactive Black 5, under alkaline-saline conditions. Free and immobilised T. versicolor cells showed 100 % decolourisation in the growth medium supplemented with 15 g l(-1) NaCl...

journal_title：Current microbiology

authors： Ottoni C,Lima L,Santos C,Lima N

更新日期：2014-01-01 00:00:00

abstract：:Bacteria acquire new DNA in a process known as horizontal gene transfer (HGT). To investigate the evolutionary impact of this transfer of DNA, various methods have been developed to detect past HGT events. For example, codon usage-based methods detect the presence of transferred genes by identifying atypical patterns ...

journal_title：Current microbiology

authors： Friedman R,Ely B

更新日期：2012-11-01 00:00:00

abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...

journal_title：Current microbiology

authors： Liu Z,Xie Y,Zhang X,Hu X,Li Y,Ding X,Xia L,Hu S

更新日期：2016-04-01 00:00:00

abstract：:The mutation of Agrobacterium radiobacter cells exposed to chrysotile asbestos was examined by the random amplified polymorphic DNA (RAPD) method. Approximately 1.4 kbp of DNA in A. radiobacter, which was not amplified strongly in the cells that were not exposed to asbestos, was amplified in the cells that were expose...

journal_title：Current microbiology

authors： Yoshida N,Naka T,Sengoku T,Ogawa K

更新日期：2001-06-01 00:00:00

abstract：:Lactobacilli are members of the normal mucosal microflora of most animals. Many isolates of Lactobacillus spp. are adherent to epithelial cells. In this study, using Lactobacillus acidophilus and L. agilis, we detected adherence in a pattern that suggested that the bacteria were binding to extracellular matrix protein...

journal_title：Current microbiology

authors： Kapczynski DR,Meinersmann RJ,Lee MD

更新日期：2000-08-01 00:00:00

abstract：:Sequence analysis of dnaE, hlyA, and asd housekeeping genes were used to determine the genetic relatedness of our collection of Vibrio cholerae isolated from patients and surface waters over a 5-year period in Iran. The results showed 41, 17, and 9 variable sites throughout the sequenced fragments of dnaE (837 bp), hl...

journal_title：Current microbiology

authors： Dashtbani-Roozbehani A,Bakhshi B,Pourshafie MR

更新日期：2013-07-01 00:00:00

abstract：:Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...

journal_title：Current microbiology

authors： Gong J,Shen XH,Qiu H,Chen C,Yang RG

更新日期：2011-12-01 00:00:00

abstract：:The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA,...

journal_title：Current microbiology

authors： Park J,Park W

更新日期：2011-01-01 00:00:00

abstract：:Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicr...

journal_title：Current microbiology

authors： Hébert L,Bidaud P,Goux D,Benachour A,Laugier C,Petry S

更新日期：2014-03-01 00:00:00

abstract：:Cutaneous fungal infections are common and widespread. Antifungal agents used for the treatment of these infections often have undesirable side effects. Furthermore, increased resistance of the microorganisms to the antifungal drugs becomes the growing problem. Accordingly, the search for natural antifungal compounds ...

journal_title：Current microbiology

authors： Lastauskienė E,Zinkevičienė A,Girkontaitė I,Kaunietis A,Kvedarienė V

更新日期：2014-09-01 00:00:00

abstract：:Potassium tellurite (K2TeO3) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K2TeO3 resistance in STEC is attributable to the ter gene cluster with terD an indicator of the c...

journal_title：Current microbiology

authors： Lewis GL,Jorgensen QR,Loy JD,Moxley RA

更新日期：2018-06-01 00:00:00

abstract：:A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% iden...

journal_title：Current microbiology

authors： Kang SH,Cho KK,Bok JD,Kim SC,Cho JS,Lee PC,Kang SK,Lee HG,Woo JH,Lee HJ,Lee SC,Choi YJ

更新日期：2006-04-01 00:00:00

abstract：:Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...

journal_title：Current microbiology

authors： Radice F,Orlandi V,Massa V,Cavalca L,Demarta A,Wood TK,Barbieri P

更新日期：2006-05-01 00:00:00

abstract：:In the present study, probiotic, safety and functional characteristics of eight indigenous bifidobacterial isolates were compared to identify suitable strains for functional food application. Among the isolates, six strains of Bifidobacterium longum and one each of Bifidobacterium breve and Bifidobacterium bifidum wer...

journal_title：Current microbiology

authors： Achi SC,Halami PM

更新日期：2019-03-01 00:00:00

abstract：:Corynebacterial isolates, obtained by selective and non-selective media from conjunctival swabs, were speciated by using two recently developed systems. All 25 strains from 92 individuals, as well as 12 strains from a previous similar study, belonged to lipophilic species. The significance of this unusual finding is d...

journal_title：Current microbiology

authors： von Graevenitz A,Schumacher U,Bernauer W

更新日期：2001-05-01 00:00:00

abstract：:Potassium ion pool was studied in glycolyzing Enterococcus hirae, grown at high or low alkaline pH (pH 9.5 and 8.0, respectively). Energy-dependent increase of K+ pool was lower for the wild-type cells, grown at pH 9.5, than that for the cells grown at pH 8.0. It was inhibited by N,N'-dicyclohexylcarbodiimide (DCCD). ...

journal_title：Current microbiology

authors： Trchounian A,Kobayashi H

更新日期：1998-02-01 00:00:00

abstract：:Microbial populations in indoor environments, where we live and eat, are important for public health. Various bacterial species reside in the kitchen, and refrigerators, the major means of food storage within kitchens, can be a direct source of food borne illness. Therefore, the monitoring of microbiota in the refrige...

journal_title：Current microbiology

authors： Jeon YS,Chun J,Kim BS

更新日期：2013-11-01 00:00:00

abstract：:Low-substrate regulated microaerophilic behavior (LSRMB) was observed in 10-54% of the bacteria isolated from several fresh-water lakes or ponds, subsurface soils, activated sludge, and Antarctic dry valley soils. Five Pseudomonas and two Bacillus type species showed LSRMB. A subsurface Pseudomonas jessenii strain was...

journal_title：Current microbiology

authors： Mazumder R,Pinkart HC,Alban PS,Phelps TJ,Benoit RE

更新日期：2000-08-01 00:00:00

abstract：:The outer membrane protein of Photobacterium damsela (OMP-PD) and the gene encoding for this porin protein were isolated and characterized. The deduced amino acid sequence of the OMP-PD monomer has 338 amino acids and a calculated molecular weight of 36,951 Da. This sequence includes a 22-amino acid signal peptide at ...

journal_title：Current microbiology

authors： Gribun A,Katcoff DJ,Hershkovits G,Pechatnikov I,Nitzan Y

更新日期：2004-03-01 00:00:00

abstract：:The cbbF genes of the facultative chemoautotroph Alcaligenes eutrophus H16 are part of two highly homologous cbb operons. Both the chromosomal and the megaplasmid pHG1-borne copy of cbbF were cloned and sequenced. Subsequent analyses including comparison with known sequences from other organisms and heterologous expre...

journal_title：Current microbiology

authors： Yoo JG,Bowien B

更新日期：1995-07-01 00:00:00

abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...

journal_title：Current microbiology

authors： Bhatti M,MacRobert A,Henderson B,Wilson M

更新日期：2002-08-01 00:00:00