Cloning of Schizosaccharomyces pombe bio2 by heterologous complementation of a Saccharomyces cerevisiae mutant.

Abstract:

:A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterization of a novel bio gene: bio2, encoding biotin synthase from Schizosaccharomyces pombe.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Phalip V,Lemoine Y,Jeltsch JM

doi

10.1007/s002849900470

keywords:

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

348-0350

issue

6

eissn

0343-8651

issn

1432-0991

pii

3072

journal_volume

39

pub_type

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