Mutants of the cyanobacterium anabaena sp. PCC 7120 altered in nitrate transport and reduction

Abstract:

:Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pleiotropic behavior. Kinetics of nitrate uptake system exhibited K(s) values of 31-38 &mgr;M for parent, SP7, and SP17 strains; however, mutant SP9 exhibited a high K(s) value of 109.5 &mgr;M. Defective nitrate reductase was apparent in mutant SP7 and SP9, while mutant SP17 exhibited partial defective nature. Methyl viologen-dependent NR activity in parent strain presented a biphasic nature with K(m) values of 0.13 and 2.47 mM, whereas a single K(m) value (2.96 mM) was observed for mutant SP17. Mutant SP9 was also defective in nitrite uptake and reduction. Mutant strains exhibited derepressed nitrogenase activity in the presence of nitrate, while glutamine synthetase activity remained unaltered.http://link.springer-ny. com/link/service/journals/00284/bibs/39n5p237.html

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Rai AK,Tiwari SP

doi

10.1007/s002849900452

keywords:

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

237-43

issue

5

eissn

0343-8651

issn

1432-0991

pii

3043

journal_volume

39

pub_type

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