Abstract:
:Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pleiotropic behavior. Kinetics of nitrate uptake system exhibited K(s) values of 31-38 &mgr;M for parent, SP7, and SP17 strains; however, mutant SP9 exhibited a high K(s) value of 109.5 &mgr;M. Defective nitrate reductase was apparent in mutant SP7 and SP9, while mutant SP17 exhibited partial defective nature. Methyl viologen-dependent NR activity in parent strain presented a biphasic nature with K(m) values of 0.13 and 2.47 mM, whereas a single K(m) value (2.96 mM) was observed for mutant SP17. Mutant SP9 was also defective in nitrite uptake and reduction. Mutant strains exhibited derepressed nitrogenase activity in the presence of nitrate, while glutamine synthetase activity remained unaltered.http://link.springer-ny. com/link/service/journals/00284/bibs/39n5p237.html
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Rai AK,Tiwari SPdoi
10.1007/s002849900452keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
237-43issue
5eissn
0343-8651issn
1432-0991pii
3043journal_volume
39pub_type
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