Abstract：
:Glutamine synthetase (GS) fromBeneckea alginolytica strain 90 was purified to homogeneity as indicated by sodium dodecyl sulface polyacrylamide gel electrophoresis. The purified enzyme was injected into rabbits, and the antiserum, as well as previously prepared antiserum against the GS fromEscherichia coli, was used in Ouchterlony double diffusion experiments. From the results of these studies, the marine and terrestrial enterobacteria could be separated into five major groups consisting of (i)Beneckea and related organisms, (ii)Photobacterium phosphoreum, P. leiognathi, andP. angustum, (iii) species ofAeromonas, (iv)Xenorhabdus luminescens, and (v) species of terrestrial enterobacteria. Evidence is presented that inB. alginolytica, as in other Gramnegative eubacteria, GS activity is regulated by adenylylation and deadenylylation.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Baumann L,Baumann Pdoi
10.1007/BF02602446subject
Has Abstractpub_date
1980-07-01 00:00:00pages
191-6issue
4eissn
0343-8651issn
1432-0991pii
10.1007/BF02602446journal_volume
3pub_type
杂志文章abstract：:We have shown previously that Spo0A approximately P-dependent sinIR operon expression was substantially down-regulated in abrB null mutant backgrounds. In this report, we show that loss of function mutations in abrB also cause phosphorelay gene expression to be down regulated. abrB null mutations caused diminished veg...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4186-2
更新日期：2004-04-01 00:00:00
abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010186
更新日期：2001-02-01 00:00:00
abstract：:The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9643-0
更新日期：2010-12-01 00:00:00
abstract：:Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9602-9
更新日期：2010-10-01 00:00:00
abstract：:Sequence analysis of dnaE, hlyA, and asd housekeeping genes were used to determine the genetic relatedness of our collection of Vibrio cholerae isolated from patients and surface waters over a 5-year period in Iran. The results showed 41, 17, and 9 variable sites throughout the sequenced fragments of dnaE (837 bp), hl...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0324-7
更新日期：2013-07-01 00:00:00
abstract：:The regulation of the recA gene expression in the obligately anaerobic rumen bacterium Prevotella ruminicola was investigated by monitoring the recA-specific transcript level. P. ruminicola recA forms a monocistronic unit, but no SOS-box sequences resembling those of Escherichia coli or Bacillus subtilis can be identi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900306
更新日期：1998-05-01 00:00:00
abstract：:In the last few years, numerous cases of multidrug-resistant Achromobacter xylosoxidans infections have been documented in immunocompromised and cystic fibrosis patients. To gain insights into the molecular mechanisms and mobile elements related to multidrug resistance in this bacterium, we studied 24 non-epidemiologi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0213-5
更新日期：2012-12-01 00:00:00
abstract：:A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase ch...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900035
更新日期：1996-04-01 00:00:00
abstract：:Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0070-7
更新日期：2012-04-01 00:00:00
abstract：:Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and construct...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0500-9
更新日期：2014-04-01 00:00:00
abstract：:A novel bacterium, designated U33(T), was isolated from a soil sample collected in Mykhailyky, Poltavs'ka oblast, Ukraine. The bacterium was aerobic, Gram-positive, spore-forming, and consists of motile rods. The taxonomic position of strain U33(T) was studied by a polyphasic approach, and the results clearly showed t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0930-7
更新日期：2016-02-01 00:00:00
abstract：:The gene organization and nucleotide sequence of the type A and B BoNT-gene clusters in Clostridium botulinum strain NCTC 2916 were studied. The aim was to clarify the organization of genes within C. botulinum type A strains possessing an unexpressed BoNT/B gene. The BoNT/A-gene cluster includes genes encoding BoNT, N...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900299
更新日期：1998-04-01 00:00:00
abstract：:Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0483-6
更新日期：2014-03-01 00:00:00
abstract：:The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacte...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010222
更新日期：2001-05-01 00:00:00
abstract：:The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment sam...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0056-5
更新日期：2012-03-01 00:00:00
abstract：:In this article, we investigated the effect of exogenous calcium on improving viability of Debaryomyces hansenii and Pichia membranaefaciens under heat stress, and evaluated the role of calcium in reducing oxidant damage of proteins in the yeast cells. The results indicated that high concentration of exogenous calcium...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0133-4
更新日期：2012-08-01 00:00:00
abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
abstract：:Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an alpha-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known alpha-amylases ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0600-x
更新日期：2007-08-01 00:00:00
abstract：:Mycoplasma penetrans invades HeLa cells and survives within them for prolonged periods of time. The intracellular distribution of M. penetrans within HeLa cells was studied utilizing the acidotropic dye LysoTracker (green), which permeates cell membranes and upon protonation remains trapped in acidic compartments. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4298-3
更新日期：2004-08-01 00:00:00
abstract：:In the present study, probiotic, safety and functional characteristics of eight indigenous bifidobacterial isolates were compared to identify suitable strains for functional food application. Among the isolates, six strains of Bifidobacterium longum and one each of Bifidobacterium breve and Bifidobacterium bifidum wer...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1615-9
更新日期：2019-03-01 00:00:00
abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0977-5
更新日期：2016-04-01 00:00:00
abstract：:The psychrotrophic bacterium Aeromonas hydrophila 7966 was subjected to cold shocks from 30 degrees C to 20 degrees C, 15 degrees C, 10 degrees C, or 5 degrees C, or were incubated at low temperature to determine its adaptative response. The cell protein patterns analyzed by two-dimensional electrophoresis revealed th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4277-8
更新日期：2004-08-01 00:00:00
abstract：:Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9585-6
更新日期：2010-08-01 00:00:00
abstract：:Despite their ubiquity, relatively few bacteriophages have been characterized. Here, we set out to explore Caulobacter bacteriophages (caulophages) in the rhizosphere and characterized Kronos, the first caulophage isolated from the rhizosphere. Kronos is a member of the Siphoviridae family since it has a long flexible...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01656-1
更新日期：2019-05-01 00:00:00
abstract：:Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgr1p depletion on cell growth. Tagging Cgr1p with the gre...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010180
更新日期：2001-01-01 00:00:00
abstract：:Cadmium (Cd) and zinc (Zn) are environmental pollutants affecting both soil and water. The toxicity resulting from the exposure of Xanthomonas campestris, a soil bacterium and plant pathogen, to these metals was investigated. Pretreatment of X. campestris with sub-lethal concentrations of Cd induced adaptive protectio...
journal_title：Current microbiology
pub_type： 新闻
doi：10.1007/s00284-003-4025-5
更新日期：2003-09-01 00:00:00
abstract：:The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylor...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4244-4
更新日期：2004-07-01 00:00:00
abstract：:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0910-y
更新日期：2016-01-01 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen, which can cause severe urinary tract infections (UTIs). Because of the high intrinsic antibiotic resistance of P. aeruginosa and its ability to develop new resistances during antibiotic treatment, these infections are difficult to eradicate. The antibiotic sus...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0026-y
更新日期：2012-01-01 00:00:00
abstract：:Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) an...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01922-7
更新日期：2020-07-01 00:00:00
