Abstract：
:Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and constructed a recombinant plasmid pJS700-hGH which carries a recombinant gene cotC-hgh with an enterokinase site under the control of cotC promoter. Plasmid pJS700-hGH was transformed into Bacillus subtilis by double crossover and an amylase-inactivated mutant was produced. After spore formation, Western blot and fluorescence immunoassay were used to monitor hGH surface expression on spores. Oral administration to silkworm with spores displaying hGH further showed that the recombinant spores may have potential ability to be digested and absorbed into the silkworm's hemolymph due to both the resistant characters of spores and the addition of enterokinase site.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Lian C,Zhou Y,Feng F,Chen L,Tang Q,Yao Q,Chen Kdoi
10.1007/s00284-013-0500-9subject
Has Abstractpub_date
2014-04-01 00:00:00pages
463-71issue
4eissn
0343-8651issn
1432-0991journal_volume
68pub_type
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