Abstract：
:The nucleotide sequences of 16S rDNAs (coding for the small subunit ribosomal RNAs) were used to identify Xylella fastidiosa, a nutritionally fastidious plant pathogenic bacterium. The near-complete 16S rDNAs from nine strains of Xyl. fastidiosa, including seven pathotypes and one strain of Xanthomonas campestris pv. campestris, were amplified through PCR with two conserved primers (forward primer 5'-AGA GTT TGA TCC TGG CTC AG-3' and reverse primer 5'-AAG GAG GTG ATC CAG CC-3') and sequenced. The 16S sequences were compared with all eukaryote and prokaryote DNA entries in GenBank database. A Xyl. fastidiosa 16S rDNA sequence, M26601, was determined to be the most similar to all the near-complete (1537 bp) and partial 5' end sequences from Xyl. fastidiosa, but not those from the Xanthomonas strain. A 20-bp oligonucleotide (5'-TTG GTA GTA ATA CCA TGG GT-3') was found to be highly characteristic of Xyl. fastidiosa. Since the 16S rDNA of Xyl. fastidiosa strains are highly homologous and characteristically different from other bacteria, including the most closely related Xanthomonas, 16S rDNA sequences can be used as signature characters to identify this bacterium.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Chen J,Banks D,Jarret RL,Chang CJ,Smith BJdoi
10.1007/s002849910006keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
29-33issue
1eissn
0343-8651issn
1432-0991pii
10006journal_volume
40pub_type
杂志文章abstract：:Grasslands are suffering from long-term overgrazing because of the population inflation. Furthermore, nitrous oxide (N2O) is a major greenhouse gas that also depletes stratospheric ozone. However, the emission rate of grassland N2O and underlying mechanisms remained unclear under different grazing intensities. We cond...
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journal_title：Current microbiology
pub_type： 杂志文章
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journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：2001-03-01 00:00:00
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更新日期：2001-08-01 00:00:00
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journal_title：Current microbiology
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：1995-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010138
更新日期：2000-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9179-8
更新日期：2008-08-01 00:00:00
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journal_title：Current microbiology
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更新日期：2006-12-01 00:00:00
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