Abstract：
:Mycoplasma penetrans invades HeLa cells and survives within them for prolonged periods of time. The intracellular distribution of M. penetrans within HeLa cells was studied utilizing the acidotropic dye LysoTracker (green), which permeates cell membranes and upon protonation remains trapped in acidic compartments. The excitation and emission spectra of the green LysoTracker are suitable for colocalization studies with rabbit anti- M. penetrans antibodies and red Cy5 goat anti-rabbit IgG. The images collected by confocal laser scanning microscopy revealed that in the infected HeLa cells almost all Cy5 fluorescent foci (red) were located within the LysoTrack-labelled intracellular compartments, apparently endosomes. Viable mycoplasmas were detected within endosomes for prolonged periods of time, apparently due to a potent antioxidant activity detected in M. penetrans.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Tarshis M,Yavlovich A,Katzenell A,Ginsburg I,Rottem Sdoi
10.1007/s00284-004-4298-3keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
136-40issue
2eissn
0343-8651issn
1432-0991journal_volume
49pub_type
杂志文章abstract：:Two strains of the well-known insect pathogen and biopesticide, Bacillus thuringiensis (Bt), belonging to subspecies alesti (strain Bt5) and kurstaki (strain Bt213), were chosen for genetic characterization. The two strains belong to different serotypes and are currently classified into different subspecies, although ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0055-z
更新日期：2002-02-01 00:00:00
abstract：:In this study, vegetative insecticidal proteins vip3 genes from Bacillus thuringiensis strains were detected based on polymerase chain reaction-high resolution melt (PCR-HRM) analysis. A pair of primers was designed according to the conservative sequences in 150 bp region of the known vip3 subfamily. The 150 bp region...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0092-9
更新日期：2012-05-01 00:00:00
abstract：:The first cloning and characterization of the gene katA, encoding the major catalase (KatA), from Xanthomonas is reported. A reverse genetic approach using a synthesized katA-specific DNA probe to screen a X. campestris pv. phaseoli genomic library was employed. A positively hybridizing clone designated pKat29 that co...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3812-8
更新日期：2003-02-01 00:00:00
abstract：:Biofilm-forming ability is well established as an important virulence factor. However, there are no studies available regarding biofilm formation of Salmonella Typhimurium 1,4,[5],12:i:-, the new pandemic serovar in Europe. To address this problem, biofilm expression by Salmonella 1,4,[5],12:i:- was evaluated using 13...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0523-x
更新日期：2014-05-01 00:00:00
abstract：:The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1402-z
更新日期：2018-04-01 00:00:00
abstract：:Saliva is a complex fluid that possesses many important functions regarding oral health. Many in vitro studies require relatively large quantities of saliva. While natural saliva would be the material of choice, it is difficult to obtain in sufficient quantities and varies in composition. Substitutes mimicking the phy...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9937-x
更新日期：2011-07-01 00:00:00
abstract：:This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4491-z
更新日期：2005-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00296203
更新日期：1995-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0114-5
更新日期：2002-09-01 00:00:00
abstract：:Lipids contribute significantly to the pathogenesis of fungal infectious diseases and an understanding of lipid metabolism occurring in fungal pathogens can help the development of more efficient antifungal therapeutic strategies. In this study, the effect of culture age on the distribution of fatty acids among differ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9052-1
更新日期：2008-02-01 00:00:00
abstract：:Potassium ion pool was studied in glycolyzing Enterococcus hirae, grown at high or low alkaline pH (pH 9.5 and 8.0, respectively). Energy-dependent increase of K+ pool was lower for the wild-type cells, grown at pH 9.5, than that for the cells grown at pH 8.0. It was inhibited by N,N'-dicyclohexylcarbodiimide (DCCD). ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900289
更新日期：1998-02-01 00:00:00
abstract：:Thiothrix spp., sulfide-oxidizing filamentous bacteria, were found to be a principal bacterial component of aquatic biofilms causing biofouling in selected municipal water storage tanks, private wells, and drip irrigation systems in Florida. Treatments of up to 200 ppm chlorine in the affected systems could not preven...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900233
更新日期：1997-09-01 00:00:00
abstract：:The chromosomal DNA of a variety of legionellae, including clinical, environmental, and plasmid-containing strains, was examined for evidence of Dam-like methylation. It was found that Dam-like methylation was present in all the strains examined regardless of their origin or plasmid content. ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900011
更新日期：1996-02-01 00:00:00
abstract：:Haloarchaea are known for its adaptation in extreme saline environment. Halophilic archaea produces carotenoid pigments and proton pumps to protect them from extremes of salinity. Bacteriorhodopsin (bR) is a light-driven proton pump that resides in the membrane of haloarchaea Halobacterium salinarum. The photocycle of...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-017-1271-5
更新日期：2017-08-01 00:00:00
abstract：:This study investigated the antifungal effects of cement paste containing Paenibacillus polymyxa E681 against Aspergillus niger, a deleterious fungus commonly found in cement buildings and structures. To test the antifungal effects, cement paste containing P. polymyxa E681 was neutralized by CO2 gas, and the fungal gr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0604-x
更新日期：2014-10-01 00:00:00
abstract：:We studied the usefulness of flow cytometry for detection of vancomycin resistance in Enterococcus faecalis by direct binding of commercially available fluorescent vancomycin to cells obtained from culture. The cells were stained with Vancomycin@FL, sonicated and additionally stained with propidium iodide (PI). Regard...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9628-z
更新日期：2010-11-01 00:00:00
abstract：:Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0349-2
更新日期：2007-07-01 00:00:00
abstract：:This study was taken up with a view to generate basic information on spore hardiness to ethanol in various Bacillus species and related genera, and to assess the effectiveness of different levels of ethanol as a bacterial disinfectant. Predominantly spore-bearing cultures of five Bacillus spp. (B. pumilus, B. subtilis...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0040-0
更新日期：2012-02-01 00:00:00
abstract：:The facultative anaerobe Pantoea sp. strain A-13, isolated from ornithogenic soil of Dewart Island (Frazier Islands), Antarctica, produced glycolipid biosurfactants when grown on n-paraffins or kerosene as the sole source of carbon and energy. Hemolysis of erythrocytes, growth inhibition of Bacillus subtilis, and thin...
journal_title：Current microbiology
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doi：10.1007/s00284-006-0345-6
更新日期：2007-02-01 00:00:00
abstract：:The yeast Candida glycerinogenes produces a high yield of glycerol only in response to a medium-osmotic stress, but little is known about the relationship between osmoadaptation and glycerol metabolism. The CgTPI gene encoding triose phosphate isomerase of C. glycerinogenes was cloned and sequenced, and its functional...
journal_title：Current microbiology
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doi：10.1007/s00284-007-0070-9
更新日期：2007-08-01 00:00:00
abstract：:The outer membrane protein of Photobacterium damsela (OMP-PD) and the gene encoding for this porin protein were isolated and characterized. The deduced amino acid sequence of the OMP-PD monomer has 338 amino acids and a calculated molecular weight of 36,951 Da. This sequence includes a 22-amino acid signal peptide at ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4111-8
更新日期：2004-03-01 00:00:00
abstract：:The effect of disodium fumarate (DF) on the ruminal fermentation profiles, the accumulation of lipopolysaccharide (LPS) and bioamines, and the composition of the ruminal bacterial community was investigated by in vitro rumen fermentation. The addition of DF increased the total gas production; the concentrations of pro...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1322-y
更新日期：2017-11-01 00:00:00
abstract：:This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02212-y
更新日期：2020-12-01 00:00:00
abstract：:Marine subsurface sediments represent a novel archaeal biosphere with unknown physiology. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of active archaea in a sediment core were ch...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0320-y
更新日期：2013-07-01 00:00:00
abstract：:A gene argH, encoding argininosuccinate lyase (ASL), has been cloned from a cosmid library of the filamentous cyanobacterium Nostoc sp. strain PCC 73102. The argH open reading frame encodes a protein comprised of 461 amino acids with a calculated molecular mass of 51,349 Da. Protein sequence comparisons reveal signifi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010298
更新日期：2001-10-01 00:00:00
abstract：:Stenotrophomonas maltophilia is an emerging opportunistic pathogen linked not only to bacteremia, sepsis, and pneumonia but also to severe chronic enteritis. Persons with the impaired immune system are prone to be infected by S. maltophilia since its pathogenicity seems to be more associated with the host immune syste...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1559-0
更新日期：2018-12-01 00:00:00
abstract：:The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01898-4
更新日期：2020-06-01 00:00:00
abstract：:The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9107-y
更新日期：2008-05-01 00:00:00
abstract：:Recently, we showed that the southern green stink bug (SGSB), Nezara viridula (L.), can transmit Pantoea agglomerans (Ewing and Fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. Here, we hypothesized that our established model could be used to determine if the SGSB was a general, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9452-5
更新日期：2009-10-01 00:00:00
abstract：:The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF02601668
更新日期：1978-01-01 00:00:00
