Abstract:
:Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cadA or cadC/ cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Nerey Md Mdel C,Pichuantes SE,Saavedra CP,Araya MA,Tantaleán JC,Vásquez CCdoi
10.1007/s00284-001-0114-5keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
187-90issue
3eissn
0343-8651issn
1432-0991journal_volume
45pub_type
杂志文章abstract::By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00294703
更新日期:1995-12-01 00:00:00
abstract::Pigment from the endophyte Burkholderia sp. WYAT7 isolated from the medicinal plant Artemisia nilagirica (Clarke) Pamp. was extracted. The antibacterial efficacy of the crude pigment Y was assessed as a source of antibiotic against both Gram-positive and Gram-negative bacterial pathogens. The pigment Y exhibited a sig...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02013-3
更新日期:2020-09-01 00:00:00
abstract::The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9239-0
更新日期:2008-12-01 00:00:00
abstract::The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01898-4
更新日期:2020-06-01 00:00:00
abstract::In this study, vegetative insecticidal proteins vip3 genes from Bacillus thuringiensis strains were detected based on polymerase chain reaction-high resolution melt (PCR-HRM) analysis. A pair of primers was designed according to the conservative sequences in 150 bp region of the known vip3 subfamily. The 150 bp region...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0092-9
更新日期:2012-05-01 00:00:00
abstract::Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900452
更新日期:1999-11-01 00:00:00
abstract::From an undisturbed natural forest soil in Ecuador, three fungal strains of the genus Aspergillus were isolated. Based on molecular and morphological features they are described as three new species, named A. quitensis, A. amazonicus, and A. ecuadorensis. ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9178-9
更新日期:2008-09-01 00:00:00
abstract::On the basis of the pulsed field gel electrophoresis(PFGE) technique, molecular karyotypes in Amoeboaphelidiumprotococcarum (the endotrophic parasite of chlorophycean algae, whichcombines protozoon- and fungus-type characters) were determined. Molecularkaryotypes in the strains X1, X5, and X31, which differ in the hos...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900155
更新日期:1997-02-01 00:00:00
abstract::Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9262-1
更新日期:2009-01-01 00:00:00
abstract::Seventy-eight bacterial isolates from human, animal, and plant hosts, representing eight species of the family Enterobacteriaceae, were screened for F-like plasmid sequences. Of the examined human Escherichia coli strains, 28% harbored one or two of the three F-like, RepFI replication regions, while 35% of the examine...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0039-7
更新日期:2002-04-01 00:00:00
abstract::A new cry gene (cry1Ca9) was cloned and sequenced from a Bacillus thuringiensis isolate native to Taiwan (G10-01A). The cry1C-type gene, designated cry1Ca9, consisted of an open reading frame of 3,567 bp, encoding a protein of 1,189 amino acid residues. The polypeptide has the deduced amino acid sequences predicting m...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3982-4
更新日期:2003-10-01 00:00:00
abstract::The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF02601668
更新日期:1978-01-01 00:00:00
abstract::Candida galli strain PGO6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. The products were confirmed by thin-layer chromatography (TLC), changes in the UV absorption pattern and high-performance liquid chr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9815-y
更新日期:2011-03-01 00:00:00
abstract::Cadmium (Cd) and zinc (Zn) are environmental pollutants affecting both soil and water. The toxicity resulting from the exposure of Xanthomonas campestris, a soil bacterium and plant pathogen, to these metals was investigated. Pretreatment of X. campestris with sub-lethal concentrations of Cd induced adaptive protectio...
journal_title:Current microbiology
pub_type: 新闻
doi:10.1007/s00284-003-4025-5
更新日期:2003-09-01 00:00:00
abstract::We constructed recombinant phage particles displaying the Bacillus thuringiensis Cry1Ba4 active toxin using the pfUSE5 and pComb3X phagemid vectors. The recombinant phage particles were screened and evaluated for displayed biologically active Cry1Ba4 toxin against the target insect larvae. Concurrent expression of Cry...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0164-9
更新日期:2006-11-01 00:00:00
abstract::The neurotoxin gene of non-proteolytic Clostridium botulinum type B (strain Eklund 17B) was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated with primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 3' end of the gene was obtained by using primer...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01569055
更新日期:1994-02-01 00:00:00
abstract::A 11.2-kb fragment containing the ectABC genes of the biosynthetic pathway of ectoine from the Gram-positive, moderately halophilic bacterium Halobacillus dabanensis D-8(T) was obtained by inverse polymerase chain reaction. Subsequently, the entire ectABC cluster was cloned and analyzed. It revealed that the intergeni...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0396-0
更新日期:2006-09-01 00:00:00
abstract::We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4103-8
更新日期:2003-10-01 00:00:00
abstract::Antimicrobial-resistant Klebsiella pneumoniae represent a global public health concern. K. pneumoniae strains isolated during 2010 and 2014-2016 within a single hospital of Molise Region, Central Italy, were analyzed testing antimicrobial susceptibility, clonality by pulsed-field gel electrophoresis (PFGE) and random ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1475-3
更新日期:2018-08-01 00:00:00
abstract::The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9977-2
更新日期:2011-09-01 00:00:00
abstract::A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1553-6
更新日期:2018-11-01 00:00:00
abstract::Microbial populations in indoor environments, where we live and eat, are important for public health. Various bacterial species reside in the kitchen, and refrigerators, the major means of food storage within kitchens, can be a direct source of food borne illness. Therefore, the monitoring of microbiota in the refrige...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0401-y
更新日期:2013-11-01 00:00:00
abstract::The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region we...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0578-8
更新日期:2014-09-01 00:00:00
abstract::Forty-eight isolates resistant to at least two antibiotics were selected from 53 antibiotic-resistant enterococci from chicken and pig meat and faeces and analysed for specific resistance determinants. Of the 48 multidrug-resistant (MDR) strains, 31 were resistant to two antibiotics (29 to erythromycin and tetracyclin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9880-x
更新日期:2011-05-01 00:00:00
abstract::To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1013-0
更新日期:2016-06-01 00:00:00
abstract::In laboratory cultures of ice nucleation-active (Ice+) Erwinia herbicola isolates, it has been difficult to achieve high-level expression of ice nuclei, especially nuclei active at temperatures warmer than -5 degrees C (i.e., type 1 ice nuclei). Here we demonstrate that starvation for phosphate and exposure to low tem...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900325
更新日期:1998-06-01 00:00:00
abstract::Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01737-1
更新日期:2019-10-01 00:00:00
abstract::In eukaryotes, loss of cytoplasmic ribosomal proteins (RPs) results in a reduced growth rate and other phenotypic defects. The ability to transition from a unicellular budding yeast to a filamentous form is very important for biofilm formation and virulence in Candida albicans. Our recent study found that loss of the ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1019-7
更新日期:2016-06-01 00:00:00
abstract::The interactions between the proteolytic X2L strain of Oenococcus oeni and the non-proteolytic 12p strain of Pediococcus pentosaceus were assayed. The characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. O. oeni showed poor cell grow...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0079-x
更新日期:2006-02-01 00:00:00
abstract::The objective of this study was to evaluate the ability of the human colonic bacterium Bacteroides ovatus V975 to ferment fenugreek fiber (Fenufibers), psyllium husk (Metamucil), and wheat bran (Wheat Chex). Strain V975 was incubated in basal medium that contained 0.1 g of each fiber source for 0, 24, or 48 h. Little ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900450
更新日期:1999-10-01 00:00:00