Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride.

Abstract:

:Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cadA or cadC/ cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Nerey Md Mdel C,Pichuantes SE,Saavedra CP,Araya MA,Tantaleán JC,Vásquez CC

doi

10.1007/s00284-001-0114-5

keywords:

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

187-90

issue

3

eissn

0343-8651

issn

1432-0991

journal_volume

45

pub_type

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