Abstract:
:The yeast Candida glycerinogenes produces a high yield of glycerol only in response to a medium-osmotic stress, but little is known about the relationship between osmoadaptation and glycerol metabolism. The CgTPI gene encoding triose phosphate isomerase of C. glycerinogenes was cloned and sequenced, and its functionality was confirmed by complementation of Saccharomyces cerevisiae tpi1 Delta. The roles of CgTpip in the glycerol biosynthesis and the osmoadaptation were investigated. Unlike S. cerevisiae tpi1 Delta and Klyuveromyces lactis tpi1 Delta, the mutant lacking CgTPI significantly decreased the rate of glucose consumption and the glycerol yield. Furthermore, the mutants decreased osmotolerance to glucose and NaCl. The results suggest that CgTPI might be crucial for a high yield of glycerol by C. glycerinogenes. The inhibition of glycerol biosynthesis might be related to the reduced ability of osmoadaptation to high external osmolarity. To our knowledge, this is the first report that inactivation of a yeast TPI gene inhibits the biosynthesis of glycerol.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Yongguang Z,Wei S,Zhiming R,Huiying F,Jian Zdoi
10.1007/s00284-007-0070-9subject
Has Abstractpub_date
2007-08-01 00:00:00pages
147-51issue
2eissn
0343-8651issn
1432-0991journal_volume
55pub_type
杂志文章abstract::Thymol (5-methyl-2-isopropylphenol) is a phenolic compound that is used to inhibit oral bacteria. Because little is known regarding the effects of this compound on ruminal microorganisms, the objective of this study was to determine the effects of thymol on growth and lactate production by the ruminal bacteria Strepto...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010145
更新日期:2000-11-01 00:00:00
abstract::The influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from Lactobacillus fermentum CRL 251 was studied. Stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; sc...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900118
更新日期:1996-11-01 00:00:00
abstract::In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9832-x
更新日期:2011-04-01 00:00:00
abstract::One of the main requirements for probiotics is their ability to survive during passage through gastrointestinal tract and to maintain their activity at different adverse conditions. The aim of the study was to look for the strains of lactobacilli and streptococci with high adhesive properties even affected by inhibito...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1017-9
更新日期:2016-06-01 00:00:00
abstract::The great increase in the abundance and phylogenetic diversity of Flavobacterium spp. within a few hundred meters downstream of the discharge site of the Westerhöfer Bach, a hardwater rivulet, raised the question whether adjacent soil may serve as a reservoir of bacteria not detected in discharge water. To address thi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9323-5
更新日期:2009-05-01 00:00:00
abstract::Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an alpha-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known alpha-amylases ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0600-x
更新日期:2007-08-01 00:00:00
abstract::A total of 216 bacterial strains were isolated from rice rhizospheric soils in Northern Thailand. The bacterial strains were initially tested for solubilization of inorganic phosphate, indole acetic acid (IAA) production, selected strains were then tested for optimized conditions for IAA production and whether these c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9674-6
更新日期:2011-01-01 00:00:00
abstract::Brevibacterium halotolerans is currently classified as a member of the Brevibacterium genus, a genus that groups together many bacterial species of similar morphology but diverse biochemical and physiological features. Here we suggest, based on multiple gene sequencing and microbial and biochemical characterization of...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1143-4
更新日期:2017-01-01 00:00:00
abstract::Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgr1p depletion on cell growth. Tagging Cgr1p with the gre...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010180
更新日期:2001-01-01 00:00:00
abstract::Lactobacillus casei CRL705 produces a class IIb bacteriocin, lactocin 705, which relies on the complementary action of two components, Lac705alpha and Lac705beta. These peptides exert a bactericidal effect on the indicator strain Lactobacillus plantarum CRL691, with an optimal Lac705alpha/Lac705beta peptide ratio of 1...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3844-0
更新日期:2003-03-01 00:00:00
abstract::The citric acid-producing fungus Aspergillus niger WU-2223L possesses a cyanide-insensitive respiratory pathway catalyzed by alternative oxidase. The regulation of the alternative oxidase under the conditions of citric acid production was determined from the transcription level of the alternative oxidase gene (aox1). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9369-z
更新日期:2009-04-01 00:00:00
abstract::In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biof...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1080-2
更新日期:2016-10-01 00:00:00
abstract::The [2Fe-2S] cluster containing ferredoxin has attracted much attention in recent years. Genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins and functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. The gene of ferred...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9025-4
更新日期:2007-12-01 00:00:00
abstract::Lipids contribute significantly to the pathogenesis of fungal infectious diseases and an understanding of lipid metabolism occurring in fungal pathogens can help the development of more efficient antifungal therapeutic strategies. In this study, the effect of culture age on the distribution of fatty acids among differ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9052-1
更新日期:2008-02-01 00:00:00
abstract::The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010111
更新日期:2000-09-01 00:00:00
abstract::The neurotoxin gene of non-proteolytic Clostridium botulinum type B (strain Eklund 17B) was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated with primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 3' end of the gene was obtained by using primer...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01569055
更新日期:1994-02-01 00:00:00
abstract::Escherichia coli (E. coli) has been widely used as a host organism for producing recombinant proteins such as biocatalysts, antibody fragments, and therapeutic hormones. To enhance recombinant protein production, many E. coli strains have been genetically engineered on practical purposes. In this study, we developed t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01730-8
更新日期:2019-11-01 00:00:00
abstract::A new cry gene (cry1Ca9) was cloned and sequenced from a Bacillus thuringiensis isolate native to Taiwan (G10-01A). The cry1C-type gene, designated cry1Ca9, consisted of an open reading frame of 3,567 bp, encoding a protein of 1,189 amino acid residues. The polypeptide has the deduced amino acid sequences predicting m...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3982-4
更新日期:2003-10-01 00:00:00
abstract::The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylor...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4244-4
更新日期:2004-07-01 00:00:00
abstract::To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900336
更新日期:1998-07-01 00:00:00
abstract::This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9038-z
更新日期:2008-01-01 00:00:00
abstract::Exposure to ethanol is a stress condition that Salmonella typhimurium often encounters during its life cycle. Food, beverage, drugs, and cosmetics have a long history of using alcohols to control pathogens. Ethanol is also commonly used for disinfecting medical instruments. This study was conducted to evaluate the eth...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9314-6
更新日期:2009-03-01 00:00:00
abstract::Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900421
更新日期:1999-08-01 00:00:00
abstract::The ability of non-tuberculous mycobacteria to form biofilms may allow for their increased resistance to currently used biocides in medical and industrial settings. This study examines the biofilm growth of Mycobacterium fortuitum and Mycobacterium marinum, using the MBEC trade mark assay system, and compares the susc...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3796-4
更新日期:2003-01-01 00:00:00
abstract::Moutai Jiuqu is a famous aromatic raw material of Maotai flavor liquor in China. It is brewed at high temperature and contains many kinds of bacteria, molds, and yeasts. There are many useful glycoside hydrolases in these microfloras, from which efficient glycoside hydrolases can be screened for biotransformation of n...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02078-0
更新日期:2020-10-01 00:00:00
abstract::By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9476-x
更新日期:2009-11-01 00:00:00
abstract::Nitric oxide synthase (NOS), the enzyme responsible for the production of endogenous nitric oxide from arginine, has been recently discovered in a number of Gram-positive bacteria. While bacterial NOS has been implicated in mediating nitrosative stress, much remains unknown about the functional role of endogenous nitr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0039-6
更新日期:2012-02-01 00:00:00
abstract::A gram-negative bacterium, designated FJY1(T), was isolated from a soil sample obtained from a university campus in South Korea. Examination showed that FJY1(T) was red-pigmented, aerobic, motile, and composed of nonspore-forming rods. This strain grew in a temperature range of 15-37 °C and a pH range of 7-9. Based on...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1105-x
更新日期:2016-11-01 00:00:00
abstract::The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9107-y
更新日期:2008-05-01 00:00:00
abstract::Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900082
更新日期:1996-08-01 00:00:00